Antioxidant and sgc-7901 cell inhibition activities of &lt;i&gt;Rhizoma Dioscoreae Bulbiferae&lt;/i&gt;. Ethanol extracts.

Chen, Xiang; Wu, Shu-Hui; Xiao-bing, Zeng; Jiang, Xu-wei; Chen, Xiaoping; Yuan, Jie; Lu, Baohua; Li, Jie

doi:10.4314/ajtcam.v10i5.7

Cited by 5 publications

(7 citation statements)

References 3 publications

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“…Studies have shown that extracts of Dioscorea bulbifera inhibit amylase and glucosidase and mediate its anti-hyperglycemic effects [73]. Other medicinal properties reported include cardio-protective, anti-oxidant, and anti-apoptotic properties [75][76][77]. In silico analysis have shown that diosgenin has an affinity to bind to amyloid peptides in monomeric and fibril form and can induce calcium influx in human cortical neuronal cell line [78,79].…”

Section: Discussionmentioning

confidence: 99%

Effect of a Novel Polyherbal Formulation on Diabetes Induced Memory Deficits in Rats

2015

Clin Exp Pharmacol

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Epidemiological, clinical and experimental studies have shown a strong correlation between Diabetes mellitus (DM) and neurodegenerative disorders. Type II diabetes mellitus (T2DM) leads to elevated levels of plasma glucose and causes neurological complications including impairment of learning and memory processes. The pathogenesis mainly includes metabolic dysfunction due to brain insulin resistance, oxidative stress, inflammation and vascular complications. In this study we explored a new pharmacological intervention by investigating the neuroprotective effects of a standardized polyherbal formulation (PF) [(Bacopa monnieri (20 mg/kg), Hippophae rhamnoides (25 mg/kg), and Dioscorea bulbifera (15 mg/kg), p.o] on passive avoidance learning (PAL) and memory in normal and streptozotocin (STZ) induced diabetic rats. Treatment was started after the onset of hyperglycemia following STZ (60 mg/kg, i.p) injection. PAL and memory were assessed 30 days after treatment, followed by retention test 24 h after training. At the beginning and end, body weights and blood glucose measurements of the animals were done. The results showed STZ induced diabetic rats had severe impairments in acquisition and retrieval processes of PAL and memory. Treatment with the PF significantly improved cognitive deficits, increased body weight and lowered plasma glucose levels in the treated as compared to untreated diabetic rats. We hypothesize that acetyl choline enhancing, antioxidant, hypoglycemic and hypolipidemic properties of phytomolecule such as bacosides, quercetin, and diosgenin in the PF might be responsible for the nootropic effects of polyherbal formulation. Our study highlights the therapeutic potential of this polyherbal formulation for multi-targeted treatment of diabetes induced cognitive impairment.

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Section: Discussionmentioning

confidence: 99%

Effect of a Novel Polyherbal Formulation on Diabetes Induced Memory Deficits in Rats

2015

Clin Exp Pharmacol

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“…Furthermore, fraction C altered the weight of spleen and thymus, and the amounts of total leukocytes, lymphocytes and neutrophils in tumour-bearing mice. Diosbulbin B was found to be the major antitumor bioactive component in the extracts and demonstrated anti-tumor effects in the dose-dependent manner at the dosage of 2 to 16 mg/kg without significant toxicity in vivo [35].…”

Section: Madagascarmentioning

confidence: 99%

"A Comprehensive Phytopharmacological Review of Dioscorea bulbifera Linn"

J¹

2017

IJESNR

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Dioscorea bulbifera Linn. is an important medicinal plant, which has long been used in traditional Indian and Chinese medicine for various diseases. This plant is pharmacologically studied for antitumor, anti HIV, antidyslipidemic, analgesic, anti-inflammatory, diuretic, gastroprotective, antioxidant, antimicrobial, antiviral, antifungal, anthelmintic, neuropharmacological, cardioprotective, anorexiant, plasmid curing activities and anti-hyperthyroid activities. A wide range of phytochemical constituents have been isolated from this plant. A comprehensive account of the morphology, microscopical characters, phytochemical constituents and pharmacological activities reported are included in view of the many recent findings of importance on this plant.

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“…Cytotoxicity test of plant extracts against T47D breast cancer cells using MTT assay was conducted following a previous procedure. 7 A volume of 100 μl suspension of T47D cell line culture containing 1.5 x 104 cells was filled in each well of 96-wells microplates. Plant extracts with a volume ranging from 50 to 400 μg/ml, with the interval of 50 μg/ml were added to the cell culture.…”

Section: Fractionation Of a Potential Extract And The Cytotoxic Activmentioning

confidence: 99%

“…The cytotoxicity of potential fractions against T47D cancer cells were carried out using a standard procedure 7 as described in the previous section.…”

Section: Mtt Assay For the Fractionsmentioning

confidence: 99%

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Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells

Nur¹,

Nugroho²

2017

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Objective: To elucidate cytotoxic activity of fractions from chloroform and methanol extracts of D. bulbifera organs on T47D breast cancer cells. Method: The vegetative organs of D. bulbifera were extracted gradually using chloroform and methanol. Cytotoxicity tested on T47D cells using MTT Assay. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) followed by thin layer chromatography (TLC). The extract and fractions potential were tested on the Vero cells using the same method as cancer cells. The most toxic fraction was analyzed using TLC followed by the application of various spray reagents for the identification of active compound. Results: The chloroform extract of the D. bulbifera leaves was the highest cytotoxic on T47D cells (IC 50 115.63±86.01 µg/mL). Moreover, the cytotoxicity test on the combined fractions of leaves chloroform extract showed that fraction 5 (F5) and fraction 6 (F6) were the most toxic fractions compared to those of other fractions. The IC 50 of both fractions were 14.55±8.62 and 7.12±4.43 µg/mL respectively. However, Its were very weak compared to those of cancer medicine (Doxorubicin) with the IC 50 was 0.04±0.02 µg/mL. Potential fractions were not toxic against Vero cells with IS>10. The active compounds in those fractions were alkaloid and terpenoid. Conclusion: Chloroform extract of the D. bulbifera leaves had the highest cytotoxic effect on T47D cells. Potential fractions were not toxic against Vero cells. The active compounds in those fractions were alkaloid and terpenoid.

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Antioxidant and sgc-7901 cell inhibition activities of <i>Rhizoma Dioscoreae Bulbiferae</i>. Ethanol extracts.

Cited by 5 publications

References 3 publications

Effect of a Novel Polyherbal Formulation on Diabetes Induced Memory Deficits in Rats

Effect of a Novel Polyherbal Formulation on Diabetes Induced Memory Deficits in Rats

"A Comprehensive Phytopharmacological Review of Dioscorea bulbifera Linn"

Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells

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