Antioxidant and Tyrosinase Inhibitory Properties of Aqueous Ethanol Extracts from Monofloral Bee Pollen

Zhang, Hongcheng; Wang, Xue; Wang, Kai; Li, Chunyang

doi:10.1515/jas-2015-0013

Cited by 25 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Schisandra chinensis bee pollen has been widely accepted as a functional food in China for several decades. Studies have confirmed its powerful antioxidant capacity among 14 species of monofloral bee pollen from China [22]. Besides this, S. chinensis bee pollen has also been reported to possess the strongest total antioxidant capacity among ten kinds of bee pollens based on Trolox equivalent antioxidant capacity, reducing power, and DPPH• radical scavenging activity [23].…”

Section: Introductionmentioning

confidence: 99%

Antioxidative and Cardioprotective Effects of Schisandra chinensis Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

et al. 2019

View full text Add to dashboard Cite

Oxidative stress plays an important role in the pathogenesis of myocardial infarction (MI). Schisandra chinensis bee pollen extract (SCBPE) possesses powerful antioxidant capacity. This study aimed to further explore the antioxidative and cardioprotective effects of SCBPE on acute MI induced by isoprenaline (ISO) in rats. The rats were intragastrically administrated with SCBPE (600, 1200, or 1800 mg/kg/day) and Compound Danshen dropping pills (270 mg/kg/day) for 30 days, then subcutaneously injected with ISO (65 mg/kg/day) on the 29th and 30th day. Compared with the model group, pretreatment with middle and high doses of SCBPE significantly reduced serum aspartate transaminase, lactate dehydrogenase, and creatine kinase activities and increased myocardial superoxide dismutase, glutathione peroxidase, and catalase activities. The histopathologic aspects showed that pathological heart change was found in the model group and reduced to varying degrees in the SCBPE groups. Moreover, the protein expression of nuclear factor-erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1), and Bcl2 in the heart increased in the SCBPE groups, while that of Bax decreased compared to the model group. Besides this, uridine was isolated from S. chinensis bee pollen for the first time. This study could provide a scientific basis for using Schisandra chinensis bee pollen as a functional food for the prevention of MI.

show abstract

Section: Introductionmentioning

confidence: 99%

Antioxidative and Cardioprotective Effects of Schisandra chinensis Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The in vitro antityrosinase activity was determined as previously reported (Zhang et al, 2015) with minor modification. Five different concentrations of the respective test sample dissolved in dimethyl sulfoxide (DMSO) were prepared.…”

Section: In Vitro Antityrosinase Activitymentioning

confidence: 99%

Safflospermidines from the bee pollen of Helianthus annuus L. exhibit a higher in vitro antityrosinase activity than kojic acid

Khongkarat

Ramadhan

Phuwapraisirisan

et al. 2020

Heliyon

View full text Add to dashboard Cite

Background: Ozone deterioration in the atmosphere has become a severe problem causing overexposure of ultraviolet light, which results in humans in melanin overproduction and can lead to many diseases, such as skin cancer and melasma, as well as undesirable esthetic appearances, such as freckles and hyperpigmentation. Although many compounds inhibit melanin overproduction, some of them are cytotoxic, unstable, and can cause skin irritation. Thus, searching for new natural compounds with antityrosinase activity and less/no side effects is still required. Here, bee pollen derived from sunflower (Helianthus annuus L.) was evaluated. Materials and methods: Sunflower bee pollen (SBP) was collected from Apis mellifera bees in Lopburi province, Thailand in 2017, extracted by methanol and sequentially partitioned with hexane and dichloromethane (DCM). The in vitro antityrosinase activity was evaluated using mushroom tyrosinase and the half maximal inhibitory concentration (IC 50 ) is reported. The antioxidation activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and reported as the half maximal effective concentration. Two pure compounds with antityrosinase activity were isolated by silica gel 60 column chromatography (SG 60 CC) and high performance liquid chromatography (HPLC), and their chemical structure deduced by Nuclear Magnetic Resonance (NMR) analysis. Results:The DCM partitioned extract of SBP (DCMSBP) had an antityrosinase activity (IC 50 , 159.4 μg/mL) and was fractionated by SG 60 CC, providing five fractions (DCMSBP1-5). The DCMSBP5 fraction was the most active (IC 50 ¼ 18.8 μg/mL) and further fractionation by HPLC gave two active fractions, revealed by NMR analysis to be safflospermidine A and B. Interestingly, both safflospermidine A and B had a higher antityrosinase activity (IC 50 of 13.8 and 31.8 μM, respectively) than kojic acid (IC 50 of 44.0 μM). However, fraction DCMSBP5 had no significant antioxidation activity, while fractions DCMSBP1-4 showed a lower antioxidation activity than ascorbic acid. Conclusion: Safflospermidine A and B are potential natural tyrosinase inhibitors.

show abstract

“…The bee pollen of Schisandra chinensis (Turcz.) Baill, which is often used as a functional food in China, has been found to possess a strong antioxidant activity [ 15 – 17 ]. Our recent study revealed the antioxidative and cardioprotective effects of S. chinensis bee pollen extract (SCBPE) on isoprenaline-induced myocardial infarction in rats [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Schisandra chinensis bee pollen’s chemical profiles and protective effect against H2O2-induced apoptosis in H9c2 cardiomyocytes

Shi

Geng

Chen

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Schisandra chinensis (Turcz.) Baill bee pollen extract (SCBPE) is often used as a functional food in China due to its good antioxidant property. However, its chemical compositions and effects on H9c2 cardiomyocytes against H2O2-induced cell injury still lacks of reports thus far. This study aimed to characterize the main components of SCBPE and investigate its protective effects against H2O2-induced H9c2 cardiomyocyte injury. Methods The main components of SCBPE were analyzed via ultraperformance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF MS/MS). The three main nucleosides in SCBPE were quantitatively analyzed via ultraperformance liquid chromatography–diode array detection. Furthermore, the potential mechanism by which SCBPE exerts protective effects against H2O2-induced H9c2 cardiomyocyte injury was explored for the first time via cell survival rate measurements; cell morphological observation; myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) and glutathione (GSH) level determination; flow cytometry; and quantitative polymerase chain reaction. Results Two carbohydrates, three nucleosides, and nine quinic acid nitrogen-containing derivatives in SCBPE were identified or tentatively characterized via UPLC–QTOF MS/MS. The nine quinic acid nitrogen-containing derivatives were first reported in bee pollen. The contents of uridine, guanosine, and adenosine were 2.4945 ± 0.0185, 0.1896 ± 0.0049, and 1.8418 ± 0.0157 μg/mg, respectively. Results of in vitro experiments showed that cell survival rate, myocardial SOD activity, and GSH level significantly increased and myocardial MDA level significantly decreased in SCBPE groups compared with those in H2O2 group. Cell morphology in SCBPE groups also markedly improved compared with that in H2O2 group. Results indicated that SCBPE protected H9c2 cardiomyocytes from H2O2-induced apoptosis by downregulating the mRNA expressions of Bax, cytochrome C, and caspase-3 and upregulating the Bcl-2 mRNA expression. Conclusions This study is the first to report that SCBPE could protect against oxidative stress injury and apoptosis in H2O2-injured H9c2 cells. Results indicated that the nucleosides and quinic acid nitrogen-containing derivatives could be the main substances that exert protective effects against H2O2-induced H9c2 cardiomyocyte injury.

show abstract

Antioxidant and Tyrosinase Inhibitory Properties of Aqueous Ethanol Extracts from Monofloral Bee Pollen

Cited by 25 publications

References 26 publications

Antioxidative and Cardioprotective Effects of Schisandra chinensis Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

Antioxidative and Cardioprotective Effects of Schisandra chinensis Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

Safflospermidines from the bee pollen of Helianthus annuus L. exhibit a higher in vitro antityrosinase activity than kojic acid

Schisandra chinensis bee pollen’s chemical profiles and protective effect against H2O2-induced apoptosis in H9c2 cardiomyocytes

Contact Info

Product

Resources

About