Antioxidant, anti-inflammatory, analgesic and in vitro-in vivo cytotoxicity effects of Spondias Venulosa (Engl.) Engl. leaf extracts on MCF-7/S0.5 and OV7 cancer cell lines

Ukwubile, Cletus Anes; Ikpefan, Emmanuel Oise; Malgwi, Troy Salvia; Dibal, Musa Yusuf; Famurewa, Ademola C.; Rasheed-Jada, Hadiza; Milagawanda, Hananiya H.; Suleiman, Abubakar Umar

doi:10.1016/j.sciaf.2021.e00917

Cited by 3 publications

(1 citation statement)

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“…For this reason, nonsteroidal anti-inflammatory drugs (NSAIDs) are the standard treatment for pains and inflammation because they inhibit the activity of cyclooxygenase enzymes, which play crucial roles in the production of prostaglandins (PGAs); endogenous mediators known to initiate pains and inflammation. The use of these NSAIDs has increased the risk of anomalies in some organs and/or tissues in the body such as the liver, kidney, GIT, and heart (Padmanabhan & Jangle, 2012;Ukwubile et al, 2021). Hence, natural product researchers have been looking for alternative herbal treatments with low side effects.…”

Section: Introductionmentioning

confidence: 99%

Ruellia simplex C. Wright (Acanthaceae): Antinociceptive, anti-inflammatory, and antidiabetic activities of a novel fatty acid isolated from its leaf extract

Ukwubile

Nettey

Malgwi

et al. 2023

ijpbp

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Ruellia simplex is a medicinal plant whose leaf is used to treat pains, inflammation, and diabetes in Nigeria. The current study was undertaken to determine the antinociceptive (analgesics), anti-inflammatory, and antidiabetic activities of a novel fatty acid isolated from the leaf extract of R. simplex. Isolation of a novel fatty acid from the most active fraction was carried out on silica gel column chromatography while, antinociceptive, anti-inflammatory, and antidiabetic activities of the isolated compound were evaluated by acetic acid, carrageenan, and alloxan-induced animal models respectively. The chemical structure of the new compound was elucidated by FT-IR, NMR, GC-MS, and LC-MS. The isolated fatty acid showed inhibition of pains by decreasing abdominal writhing in mice in dose dependent fashion as well as reduced paw volume in the carrageenan-induced paw edema in rats at IC50 = 12.5 ± 1.08 μg/ml and 10.21 ± 1.02 μg/ml, respectively, whereas the antidiabetic activity showed a dose dependent reduction in blood sugar levels with IC50 = 6.02 ± 0.01 μg/ml. The compound showed the following features: R-COOH functional group at 3327 wavelength cm -1 by FTIR; EI-MS [M] +* at m/z 467, peak area 62.231% and RT 14.086 min by GC-MS; singly charged fragments at m/z 116.1 and m/z 465.1, RT 1.31 min by LC-MS and eight proton signals consisting of singlets and multiplets ( 1 H), thirty carbon atoms ( 13 C) NMR data. From the study, the novel fatty acid from R. simplex extract was potentially active for the treatment of pains, inflammation, and diabetes.

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Section: Introductionmentioning

confidence: 99%