Antioxidant, Anti-Inflammatory, and Postulated Cytotoxic Activity of Phenolic and Anthocyanin-Rich Fractions from Polana Raspberry (Rubus idaeus L.) Fruit and Juice—In Vitro Study

Abstract

In this study, the antioxidative and anti-inflammatory potential of crude extracts (CE), anthocyanin-rich fractions (ARF), and phenolic fractions (PF) from raspberry (R) and raspberry juice (J) were evaluated. The antioxidant properties were evaluated with three complementary assays: DPPH radical scavenging activity, chelating Fe(II) power, and ferric reducing power. The highest antioxidant activity was determined for the crude extract from raspberry pulp (RCE) in the case of all methods used. The anti-inflammatory activity was demonstrated by inhibitory effect on lipoxygenase (LOX) and cyclooxygenase-2 (COX-2) activity in vitro. The highest efficiency in inhibiting the activity of both enzymes was exhibited by RCE, 0.79 and 0.59 mg FW/mL, respectively. In turn, JARF had the lowest ability to inhibit LOX (EC50 = 4.5 mg FW/mL) and JPF caused the lowest COX-2 inhibition (1.75 mg FW/mL). Additionally, we have performed a pilot study of in vitro cytotoxic activity using two human leukemia cell lines: J45 and HL60. All examined extracts inhibited the viability of J45 cells more effectively than HL60. The highest cytotoxic effect was observed in the J45.01 cell line after exposure to RCE (EC50 = 0.0375 mg FW/mL).

Caucasian endemic medicinal and nutraceutical plants: in-vitro antioxidant and cytotoxic activities and bioactive compounds

“…A number of Rubus species showed cytotoxic activity. For example, the fruits of Rubus chingii Hu showed cytotoxic activity against HepG2, Bel‐7402, A549 and MCF7 human cancer cell lines, while R. idaeus L. revealed higher cytotoxic activity towards the human leukaemia cell lines: J45 and HL60 . In the current study, R. takhtadjanii , like R. chingii, showed similar cytotoxic activity against HepG2 cell lines.…”

Flavan-3-ols Content in Red Raspberry Leaves Increases under Blue Led-Light Irradiation

“…Thus, we selected red raspberry ( Rubus idaeus L.) as our experimental model plant because it is a species widely known for its edible fruits and as polyphenol rich and highly beneficial for human health [22,23]. Furthermore, they are most commonly known as food products and their extracts are also known as anti-inflammatory [24] and antimicrobial properties [25]. As mentioned above, numerous studies report increasing polyphenol content in various plants by light irradiation management.…”

Efficacy of a phenol derivative, isopropyl vanillate, as an anti‐inflammatory agent: A new small molecule inhibitor of COX and neutrophil migration

“…In the gastric toxicity experiment, we showed that even by using a 100 times higher dose, ISP‐VT was less toxic than indomethacin. Data in the literature show that ISP‐VT natural analogue vanillic acid decreased levels of COX‐2 and inhibit activation of nuclear transcription factor‐κB (NF‐κB) (Kim et al, ; Rosa et al, ; Szymanowska, Baraniak, & Bogucka‐Kocka, ; Zduńska, Dana, Kolodziejczak, & Rotsztejn, ).…”