Antioxidant, Anti-Melanogenic and Anti-Wrinkle Effects of<i>Phellinus vaninii</i>

Im, Kyung Hoan; Baek, Seung A; Choi, Jaehyuk; Lee, Tae Soo

doi:10.1080/12298093.2019.1673595

Cited by 31 publications

(30 citation statements)

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“…To evaluate if PNPs have the inhibitory ability against collagenase, we used in vitro collagenase activity assay as an evaluation model. Epigallocatechin gallate (EGCG), the predominant catechin in tea, possesses the inhibition of collagenolytic activity by collagenase and is usually used as positive control in collagenase activity assay [40][41][42]. The collagenase inhibitory activity of PNPs and epigallocatechin gallate (EGCG) was shown in Figure 3.…”

Section: Anti-collagenase Activity Of Pnpsmentioning

confidence: 99%

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

et al. 2020

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Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 ± 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 ± 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.

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Section: Anti-collagenase Activity Of Pnpsmentioning

confidence: 99%

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

et al. 2020

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“…As presented in a previous study, the short-term germination of the grain slightly altered the total phenolic content, but dynamically changed the phenolic profiles [26]. It is well known that tyrosinase enzyme plays a legitimate role in melanogenesis and can be modulated by antioxidants [8,27]. In this study, germinated riceberry rice extract had a strong inhibitory effect on tyrosinase-related melanogenesis and also affected the morphological appearance of B16 cells by reducing cell size, cellular melanin distribution, and dendriticity.…”

Section: Discussionmentioning

confidence: 69%

“…The total phenolic content of the sample was determined by the Folin-Denis assay, as described previously [8]. Briefly, 20 µL of sample or gallic acid (Sigma-Aldrich, Singapore) were mixed with 100 µL of Folin-Denis reagent (Sigma-Aldrich, Singapore) and 1880 µL of 7.5% aqueous sodium bicarbonate solution for 30 min at room temperature.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

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Germinated Riceberry Rice Enhanced Protocatechuic Acid and Vanillic Acid to Suppress Melanogenesis through Cellular Oxidant-Related Tyrosinase Activity in B16 Cells

2020

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The anti-melanogenic bioactivities of phytophenolic compounds have been well recognized. Riceberry rice contains a rich source of phenolic compounds that act as melanin inhibitors through their antioxidant and anti-tyrosinase properties. Germination has been shown to be an effective process to improve targeted phenolic compounds. In this study, germinated riceberry rice extract was tested for antioxidant activity. Total phenolic content was determined while the tyrosinase inhibitory effect was screened by the in vitro mushroom tyrosinase assay. Cytotoxicity of germinated riceberry rice extract was investigated in B16 cells before evaluating its activities on cellular tyrosinase, melanogenesis, melanin excretion, morphological appearance, and cellular oxidants. Germinated riceberry rice extract showed increased potency of antioxidants and was also twice as effective for mushroom tyrosinase inhibition when compared with ungerminated riceberry rice extract. In B16 cells, the extract inhibited cellular tyrosinase, melanogenesis, and cellular oxidants in a dose-dependent manner when compared with untreated cells. Germinated riceberry rice extract also displayed an effect on B16 cells morphology by reducing the number of melanin-containing cells and their dendriticity. Additionally, the germination of riceberry rice dominantly enhanced two phenolic acids, protocatechuic acid and vanillic acid, which have the potential for antioxidant-associated hyperpigmentation control. Thus, the restricted germination of riceberry rice tended to promote protocatechuic acid and vanillic acid, which dominantly displayed antioxidants and tyrosinase-related melanogenic inhibition.Antioxidants 2020, 9, 247 2 of 12 acid, chlorogenic acid, syringic acid, p-coumaric acid, and ferulic acid [10,11] that act as tyrosinase inhibitors. Previously, a suitable germination process promoted both the quantitative and qualitative aspects of these valuable phenolic compounds [12][13][14][15]. In this study, riceberry rice was enhanced for anti-melanogenesis by the germination process. Germinated riceberry rice extract could be further developed as a novel agent for hyperpigmentation control. Materials and Methods Rice GerminationRiceberry rice was obtained from Phayao province, Thailand. A hundred grams of rice were soaked in reverse osmosis water, pH 7.2-7.4 for 1 h. Soaked riceberry rice was germinated in a polyethylene tray (22 × 22 × 5 cm) at room temperature in the dark for 6 h. Subsequently, germinated riceberry rice was lyophilized and then ultracentrifugally powdered. The riceberry rice powder was then sieved through 345 µm and stored at −20 • C until extraction.

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“…ROS are unstable and highly-reactive chemical species 17 . Since NADPH oxidase activation is a key enzymatic source of ROS formation 18 , and in eukaryotes, it is an important source of ROS including H 2 O 2 19 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling Identifies Candidate Genes Involved in Polyporus Umbellatus Sclerotial Formation Affected by Oxalic Acid

Xing

Zeng

et al. 2020

Preprint

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Polyporus umbellatus is a precious medicinal fungus. The whole transcriptome of P. umbellatus exposed to different concentrations of oxalic acid was performed and analyzed using RNA-seq based on sequencing technology. Reactive oxygen species (ROS) detection of P. umbellatus mycelia was visually conducted and using non-invasive micro-test technology (NMT), the net Ca2+ and H2O2 fluxes of P. umbellatus were measured. Totally, 22,523 unigenes were generated by De novo assembly of reads and there are 1223 differentially expressed genes (DEGs) between the control group and the high oxalic acid complemented (PD_S) group and 459 DEGs between the control group and the low concentration oxalic acid additive (PU_SM) group. The transcriptomic analysis indicated DEGs encoding enzymes related to oxidative stress, energy metabolism and so on. Compared to that of the control group, the biomass of the sclerotia in the PU_SM group increased 66%, however, no sclerotia formed in the PD_S group. The low concentration of oxalic acid could increase Ca2+ and H2O2 influx, while the high concentration of oxalic acid presented slight H2O2 efflux. There is a great significant positive correlation between the net Ca2+ and H2O2 fluxes. Different concentrations of exogenous oxalic acid affected P. umbellatus sclerotial formation in different ways.

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Antioxidant, Anti-Melanogenic and Anti-Wrinkle Effects ofPhellinus vaninii

Cited by 31 publications

References 50 publications

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

Germinated Riceberry Rice Enhanced Protocatechuic Acid and Vanillic Acid to Suppress Melanogenesis through Cellular Oxidant-Related Tyrosinase Activity in B16 Cells

Transcriptomic Profiling Identifies Candidate Genes Involved in Polyporus Umbellatus Sclerotial Formation Affected by Oxalic Acid

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