Plants are naturally rich sources of bioactive molecules and phytochemicals used to augment human fitness with limited side effects 1 . More than one-tenth of all plant species have found applications in cosmeceutical or pharmaceutical industries. 2 Different plant types such as climbers, aromatic plants, and trees are exploited as sources of biopharmaceutical products for treating infections 3 . Plants are rich in volatile oils, often referred to as essential oils, and these are found in the seeds, stems, leaves, or flowers and twigs. These essential oils are usually biologically active as antioxidants and antimicrobials. 4,5 Besides, plants contain phytochemicals, chiefly saponins, flavonoids, terpenoids, polysaccharides, tannins, alkaloids, and steroids that contribute to their biological activities. 6,7 Natural products can be exploited as a source of novel biomolecules, separated into bioactive fractions using different polar solvents. 8 Different medicinal plants and plant parts have been found to possess antimicrobial properties. Nicotiana tabacum commonly referred to as tobacco is a cash crop that is cultivated on a large scale worldwide. 9 The plant is utilized in the management of cancer, cough, ulcer, and respiratory tract infections. 10 Popova et al determined the bioactive constituents in seeds of N. tabacum, 11 while Al-Lahham et al. used organic extracts of the root of N. tabacum for antimicrobial, cytotoxic, and antioxidant studies. 2 The antiparasitic activities of leaf extract of N. tabacum were determined in a previous study. 12