Antioxidant, Antidiabetic, and Anti-Inflammatory Effects of Extracts and Fractions from Parthenocissus tricuspidata Stems

Abstract

This study was carried out to analyze the effects on antioxidative, antidiabetic, and anti-inflammatory activities of Parthenocissus tricuspidata (PT) stem extracts. The total phenolic contents of hot water and ethanol extracts from PT stems were 61.5 mg TAE/g and 122.1 mg TAE/g, respectively. The antioxidative activities of hot water and ethanol extracts from PT stem were measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD) assay. The DPPH radical scavenging activities of ethanol extract and butanol fraction were approximately 95% and 92% at 1 mg/ml, respectively, and the SOD activities of ethanol extract and butanol fraction were about 91% and 97% at 1 mg/ml, respectively. The DPPH radical scavenging and SOD activities of ethanol extract and butanol fraction from PT stem increased remarkably increased in a dose-dependent manner and were higher than in the hot water extracts. Compared to the acarbose, a known anti-diabetic drug, which was used as a positive control, the α-glucosidase inhibitory capacity of PT stem showed a strong inhibitory rate in ethanol extract and in butanol and hexane fractions. We investigated the effect of hot water extract from PT stem on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. Hot water extract from PT stem inhibited LPS-induced NO production up to 40% at a treatment of 1 mg/ml. These results suggest that PT stem extracts have an effect on antioxidative, antidiabetic, and anti-inflammatory activities and thus have great potential as antidiabetic materials and a source for natural health products.

Evaluation of clinical trial of atopic dermatitis by a topical cream containing the extracts from photosynthetic bacteria, Rhodobacter sphaeroides

“…Based on the above results of pH and water contents changes as well as SCORAD index, it could tell that the effects of the test topical cream for relieving atopic dermatitis may be attributed to the removal of reactive oxygen species (ROS) in the skin, which is a major cause of inflammation by the super oxide dismutase (SOD) and catalase produced by photosynthetic bacteria (Cho and Choi 2013). Therefore, it is very positive that the photosynthetic bacteria formulation in this cream showed remarkable enhancement, compared to those of commercially used topical cream for atopic dermatitis.…”

“…SOD is an anti-oxidant enzyme that has been reported to convert harmful oxygen radicals in the body into H 2 O 2 , and it has oxidation inhibitory functions via its conversion into water and oxygen by catalase to remove harmful active oxygen in vivo, thereby protecting living bodies (Cho and Choi 2013; McCord and Fridovich 1969). Although diverse studies on aging and disease have been conducted using eukaryotic organisms as materials, studies on microorganisms existing in the natural world in large numbers are insufficient.…”

Antioxidant and Anti-inflammatory Effects of Solvent Fractions from the Peel of the Native Jeju Citrus ‘Hongkyool’ and ‘Pyunkyool’

“…2) Results are mean±SD (n=3). Values followed by the same letter are not significantly different (p<0.05).…”

Antioxidant and anti-inflammatory activities of hot water extracts of Ligularia fischeri

“…따라서 천연물로부터 항산화제를 얻고자 하는 연구가 많이 진행되 고 있다. 또한 항산화 효과를 가지는 물질들이 항염증 효과 를 가질 수 있다는 연구와 연계하여 항산화 효과와 함께 함염증 효과를 가진 천연 물질들을 검토한 연구 결과도 다수 보고되어져 있다 (6)(7)(8).…”