Antioxidant, Antiproliferative, and Pro-apoptotic Capacities of Pentacyclic Triterpenes Found in the Skin of Olives on MCF-7 Human Breast Cancer Cells and Their Effects on DNA Damage

Abstract

This research aimed to investigate erythrodiol, uvaol, oleanolic acid, and maslinic acid scavenging capacities and their effects on cytotoxicity, cell proliferation, cell cycle, apoptosis, reactive oxygen species (ROS) level, and oxidative DNA damage on human MCF-7 breast cancer cell line. The results showed that erythrodiol, uvaol, and oleanolic acid have a significant cytotoxic effect and inhibit proliferation in a dose- and time-dependent manner. At 100 μM, erythrodiol growth inhibition occurred through apoptosis, with the observation of important ROS production and DNA damage, whereas uvaol and oleanolic acid growth inhibition involved cell cycle arrest. Moreover, although all tested triterpenes did not show free radical scavenging activity using ABTS and DPPH assays, they protected against oxidative DNA damage at the concentration 10 μM. Uvaol and oleanolic and maslinic acids, tested at 10 and 100 μM, also reduced intracellular ROS level and prevented H(2)O(2)-induced oxidative injury. Overall, the results suggest that tested triterpenes may have the potential to provide significant natural defense against human breast cancer.

Free radical scavenging and α-glucosidase inhibition, two potential mechanisms involved in the anti-diabetic activity of oleanolic acid

“…We have evaluated the OA ability to scavenge three radical species (ABTS+, DPPH and peroxyl), finding that it can capture ABTS+ radicals in a moderate and dose-dependent manner, but it lacks the ability to scavenge DPPH species. These results are in agreement with those previously provided by other authors (Yang et al, 2007;Wang et al, 2010;Allouche et al, 2011).…”

“…Its ability to react with diverse radical species has been evaluated, having obtained an array of varied results (Yin and Chan, 2007;Yang et al, 2007;Wang et al, 2010;Allouche et al, 2011). The OA effect on α-amylase is not completely clear (Khati et al, 2013;Wang et al, 2011).…”

Antioxidant, Antiproliferative, and Pro-Apoptotic Activities of a Saponin Extract Derived from the Roots of Panax notoginseng (Burk.) F.H. Chen

“…Cytotoxicity, a common preliminary method, is helpful to detect whether tested compounds have potential antineoplastic abilities. 27 The cytotoxic effect of the crude saponin extracts from P. notoginseng has been previously described in hepatocarcinoma (SMMC-7721), prostate cancer (PC-3). and breast cancer (MCF-7) cell lines.…”

“…27 On the one hand, cell cycle is under strict regulation in the cell with numerous control points that allow correct progression through the different phases. In this sense, a delay in progression of the G0/G1, S, or G2/M cell cycle phases would constitute a cellular defense mechanism to allow action of FIG.…”

Hematologic Toxicosis; Induced by Doxorubicin Protective Effects of Combination of Olea Europeae and Ginkgo Biloba

“…Phenolic constituents including oleuropein, hydroxytyrosol and tyrosol were responsible for most of the olive pharmacologic activities. 6 The extract in a time and dose dependent manner have decreased proliferation and possess cytotoxic effects, 7 also inhibits production of ROS, decrease in the production of Thromboxane A2, anti-inflammatory and inhibition of platelet aggregation, 8 reduces infarct size, 9 produces antioxidant effect. 6 The extract of Olea europeae put forth a defensive process by reducing the DOX cardiotoxicity which was articulated by the changes in peripheral and intracellular markers.…”