Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense

Li, Xuehui; Lu, Yongming; Zhang, Wenna; Yuan, Shixiong; Zhou, Liyuan; Wang, Liming; Ding, Qiuying; Wang, Dandan; Yang, Weiwei; Cai, Zheng-Nan; Chen, Yan

doi:10.1016/j.ijbiomac.2015.10.006

Cited by 20 publications

(3 citation statements)

References 31 publications

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“…FT‐IR successfully confirmed the sulfation process through the presence of the sulfate group in J H S . Similar results have been found in other sulfated polysaccharides [13,25,45] …”

Section: Resultssupporting

confidence: 90%

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Zhao¹,

Tian

Wang³

et al. 2021

Chemistry & Biodiversity

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AIDS, caused by HIV‐1, is one of the most dangerous infectious diseases in the world. Therefore, it is necessary to develop new drugs with more potent bioactivities, less toxicity and higher tolerability for controlling the viral load, particularly by using the raw materials that are widely available. Agaricus blazei Murill (AbM), known in China as jisongrong, is of great importance as a food source and as a health‐promoting supplement for immunomodulation. The polysaccharides of AbM exhibit various biological activities, such as regulating cellular immunity and providing anti‐oxidative, anti‐infective, and anti‐inflammatory effects. At present, to our knowledge, no report has explored the chemically sulfated and anti‐HIV‐1 activity of AbM polysaccharides. Herein, the sulfated AbM polysaccharides with different sulfur contents were prepared by the chlorosulfonic acid‐pyridine method. The characteristics of sulfated derivatives were established by the determination of the sulfur content, the relative molecular weight, and the Fourier‐transform infrared spectroscopy. The anti‐HIV activities of the sulfated AbM polysaccharides were evaluated by CCK‐8 and the single‐cycle pseudovirus infection (TZM‐bl) assay. The sulfated AbM polysaccharides had strong antiviral properties, and the half‐maximal inhibitory concentrations approached that of the positive control, azidothymidine. Sulfated modification of AbM polysaccharides can increase their anti‐HIV pharmacological activity, which makes them promising alternative candidates as bioactive macromolecules for biomedical applications in HIV/AIDS.

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“…FT‐IR successfully confirmed the sulfation process through the presence of the sulfate group in J H S . Similar results have been found in other sulfated polysaccharides [13,25,45] …”

Section: Resultssupporting

confidence: 90%

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Zhao¹,

Tian

Wang³

et al. 2021

Chemistry & Biodiversity

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“…(2) DPPH Radical Scavenging Capacity. DPPH primarily exists in the form of nitrogen-containing free radicals and can accept single electron or hydrogen atom [25]. Therefore, the stability of DPPH radical is an important indicator to evaluate biological activity.…”

Section: Antioxidant Capacity Of Corn Silk Polysaccharides With Diffementioning

confidence: 99%

Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Chen

Sun

Ouyang

2020

Oxidative Medicine and Cellular Longevity

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Corn silk polysaccharide (CSP0; molecular weight=124 kDa) was degraded by ultrasonication to obtain five degraded polysaccharides, namely, CSP1, CSP2, CSP3, CSP4, and CSP5, with molecular weights of 26.1, 12.2, 6.0, 3.5, and 2.0 kDa, respectively. The structures of these polysaccharides were characterized by FT-IR, 1H NMR, and 13C NMR analyses. The antioxidant activities, including scavenging ability for hydroxyl radicals and DPPH free radicals, chelation ability for Fe2+ ions, and reducing ability of CSP increased with decreased molecular weight of CSPs within 6.0 to 124 kDa. However, antioxidant activity weakened when the molecular weight of CSPs reached 3.5 and 2 kDa. CSP3 with a molecular weight of 6.0 kDa exhibited the strongest antioxidant activity. After protection with 60 μg/mL CSPs, the viability of human renal proximal tubular epithelial cells (HK-2) damaged by nano-COM crystals increased, the level of reactive oxygen species decreased, and the amount of COM crystal adhered onto the cell surface decreased. The ability of CSPs to protect cells from CaOx crystal damage was consistent with their antioxidant activity. CSPs can specifically combine with CaOx crystal to inhibit the conversion of calcium oxalate dihydrate crystal to calcium oxalate monohydrate crystal. All these results showed that the activity of CSPs was closely correlated with molecular weight. A very high or low molecular weight of CSPs was not conducive to their activity. CSPs, especially CSP3 with a molecular weight of 6.0 kDa, can be used as a potential antistone drug.

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“…However, fragments linked by a large amount of GalA were more stable, thus increasing the proportion of GalA [58]. In addition, numerous studies have shown that acidic polysaccharides generally have high bioactivity [59,60]. Natural and artificial selenium-enriched green teas…”

Section: Monosaccharide Compositionmentioning

confidence: 99%

Advances in the Utilization of Tea Polysaccharides: Preparation, Physicochemical Properties, and Health Benefits

et al. 2022

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Tea polysaccharide (TPS) is the second most abundant ingredient in tea following tea polyphenols. As a complex polysaccharide, TPS has a complex chemical structure and a variety of bioactivities, such as anti-oxidation, hypoglycemia, hypolipidemic, immune regulation, and anti-tumor. Additionally, it shows excellent development and application prospects in food, cosmetics, and medical and health care products. However, numerous studies have shown that the bioactivity of TPS is closely related to its sources, processing methods, and extraction methods. Therefore, the authors of this paper reviewed the relevant recent research and conducted a comprehensive and systematic review of the extraction methods, physicochemical properties, and bioactivities of TPS to strengthen the understanding and exploration of the bioactivities of TPS. This review provides a reference for preparing and developing functional TPS products.

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Antioxidant capacity and cytotoxicity of sulfated polysaccharide TLH-3 from Tricholoma lobayense

Cited by 20 publications

References 31 publications

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights

Advances in the Utilization of Tea Polysaccharides: Preparation, Physicochemical Properties, and Health Benefits

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