Antioxidant, cytotoxic and antibacterial potentials of biosynthesized silver nanoparticles using bee’s honey from two different floral sources in Saudi Arabia

Abstract

Nowadays, the innovative study of silver nanoparticles (AgNPs) is excessive since they have incredible biomedical applications. The current study aimed to find out the potential of honey from two different floral sources (Ziziphus spina-christi and Acacia gerrardii) as biogenic mediators to synthesize AgNPs and to evaluate their antioxidant, cytotoxic and antimicrobial abilities. Biogenic AgNPs were studied for particle characterizations and the expected biomolecules helped in the reduction process of silver (Ag) ions to AgNPs. Results demonstrated different size (50–98 nm) and potential −42 and −30 for AgNPs prepared using different biological materials, therefore different 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging free radicals were observed. Cytotoxic effect in a dose-dependent manner was detected against LoVo and HepG2 ca cells for biogenic AgNPs resulted from cell apoptosis that detected by caspase 3/7 activation in the treated cells compared to their corresponding controls. Furthermore, biogenic AgNPs suppressed the growth of Methicillin-resistant bacteria Staphylococcus aureus (Gram-positive) besides Escherichia coli and Peseudomonas aeruginosa (Gram-negative). The LC50 of AgNPs was between 12.8 and 19 μg/mL and the antibacterial capability was between 20.8 ± 1.2 and 15.6 ± 0.8 mm. Bacterial membrane disturbance was evident in the current study when treated bacteria were studied by field emission scanning electron microscopy (FE-SEM) in relation to untreated controls. Overall, the present findings indicated the possibility of simple green synthesis of AgNPs using honeybee, which are effective agents in some biomedical applications. Detailed future work is needed to further validate the results.

Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

“…In their study, lower IC 50 of 3.8 and 6.6 µg/mL were found compared with our results when they used AgNPs synthesized using Ganoderma lucidum and Phellinus igniarius respectively. Generally, anticancer activity of AgNPs prepared using green synthesis was well documented 16,35,58,88,89 . Further studies stated the IC 50 of AgNPs synthesized using biogenic extract were ranged between 10.9 to 21.4 against HepG2, LoVo, and MDA-MB 231cell 90 and another study showed IC 50 ranged between 35.15-56.73 μg/mL against a LoVo cell with 16 .…”

Quality evaluation of Saudi honey harvested from the Asir province by using high-performance liquid chromatography (HPLC)

“…The effect of floral origin and altitude on physiochemical properties and concentration of vitamins was analyzed by Al-Mosa et al, 2019 , Mohammed et al, 2019 . Many researchers worked on antimicrobial ( Abdallah and Hamed, 2019 , Al-Hindi and Shehata, 2014 , Ansari et al, 2017a , Ghramh et al, 2019a , Ghramh et al, 2019b , Owayss et al, 2019 ), antioxidant ( Al-Hindi and Shehata, 2014 ), physicochemical properties including some quality parameters ( Ahamed et al, 2017 , Alqarni et al, 2014 , Alqarni et al, 2016 , Mesallam and El-Shaarawy, 1987 , Osman et al, 2007 ), mineral contents ( Al-Hindi and Shehata, 2014 , Alqarni et al, 2014 , Arida et al, 2012 , Osman et al, 2007 , Taha et al, 2018 ), floral pigments ( Alqarni et al, 2016 ), heavy metals ( Bazeyad et al, 2019 ), validation of botanical origins and geographical sources through ultraviolet spectroscopy and chemo metric analysis ( Ansari et al, 2018 ), and formation and biological activities of nanoparticles by using honey samples ( Al-Brahim and Mohammed, 2020 , Ghramh et al, 2019b ). Most of the previous research on honey deals with the above-mentioned parameters rather than quantifying the two most important quality parameters (sucrose and HMF) present in honey samples.…”

Biosynthesized silver nanoparticles using Caulerpa taxifolia against A549 lung cancer cell line through cytotoxicity effect/morphological damage

“…Various routes are available in nanoparticles synthesis; they are physical, chemical, biological. Among these, biological route is considered more efficient than other routes due to the low toxicity, low cost, increased surface nature and volume zero ( Al-Brahim and Mohammed, 2020 ). Sometimes, the chemical mediated nanoparticles seriously affected the environment and others directly or indirectly due to the toxic nature ( Lakhan et al, 2020 ).…”