Antioxidant defense response during hibernation and arousal in Chinese soft-shelled turtle Pelodiscus sinensis juveniles

Zhong-hua, Tang; Chen, Bo-jian; Niu, Cuijuan

doi:10.1016/j.cryobiol.2021.01.015

Cited by 14 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GPX4 requires GSH as a reducing agent, which is a cofactor necessary for GPX4 to exert its antioxidant effects (Eleftheriadis et al, 2019). In a study on liver of soft‐shelled turtle ( Pelodiscus sinensis juveniles), Gpx4 mRNA levels were found to be significantly elevated during hibernation and returned to pre‐hibernation levels during arousal, (Tang et al, 2021) which was consistent with our findings. Another study reported that hibernating Syrian hamsters' renal tubular epithelial cells could escape lipid peroxidation‐induced cell death via increased GPX4 activity, although it was not at low temperature (Eleftheriadis et al, 2018).…”

Section: Discussionsupporting

confidence: 91%

“…Transcriptomic analysis revealed that glutathione S‐transferase mu 1 (GSTM1) and glutathione S‐transferase mu 2 (GSTM2) were significantly downregulated in the LT group as compared with the SA group, whereas GSTM1 was significantly upregulated in the IBA group. Similarly, mRNA levels of various isoforms of glutathione S‐transferase in Chinese soft‐shelled turtles ( Pelodiscus sinensis ) and ground squirrels ( Citellus citellus now known as Spermophilus citellus) were shown to remain stable or decrease during hibernation but increase in during IBA (Blagojević et al, 1998; Tang et al, 2021; Zhang et al, 2017). This supports our findings that increased binding of GSTM to glutathione may be necessary to remove metabolic wastes during the arousal period, potentially to avoid metabolic waste accumulation from oxidative stress over repeated torpor‐arousal cycles.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Integrated transcriptomics and metabolomics reveal protective effects on heart of hibernating Daurian ground squirrels

Yang,

Hao,

et al. 2023

Journal Cellular Physiology

View full text Add to dashboard Cite

Hibernating mammals are natural models of resistance to ischemia, hypoxia‐reperfusion injury, and hypothermia. Daurian ground squirrels (spermophilus dauricus) can adapt to endure multiple torpor‐arousal cycles without sustaining cardiac damage. However, the molecular regulatory mechanisms that underlie this adaptive response are not yet fully understood. This study investigates morphological, functional, genetic, and metabolic changes that occur in the heart of ground squirrels in three groups: summer active (SA), late torpor (LT), and interbout arousal (IBA). Morphological and functional changes in the heart were measured using hematoxylin‐eosin (HE) staining, Masson staining, echocardiography, and enzyme‐linked immunosorbent assay (ELISA). Results showed significant changes in cardiac function in the LT group as compared with SA or IBA groups, but no irreversible damage occurred. To understand the molecular mechanisms underlying these phenotypic changes, transcriptomic and metabolomic analyses were conducted to assess differential changes in gene expression and metabolite levels in the three groups of ground squirrels, with a focus on GO and KEGG pathway analysis. Transcriptomic analysis showed that differentially expressed genes were involved in the remodeling of cytoskeletal proteins, reduction in protein synthesis, and downregulation of the ubiquitin‐proteasome pathway during hibernation (including LT and IBA groups), as compared with the SA group. Metabolomic analysis revealed increased free amino acids, activation of the glutathione antioxidant system, altered cardiac fatty acid metabolic preferences, and enhanced pentose phosphate pathway activity during hibernation as compared with the SA group. Combining the transcriptomic and metabolomic data, active mitochondrial oxidative phosphorylation and creatine‐phosphocreatine energy shuttle systems were observed, as well as inhibition of ferroptosis signaling pathways during hibernation as compared with the SA group. In conclusion, these results provide new insights into cardio‐protection in hibernators from the perspective of gene and metabolite changes and deepen our understanding of adaptive cardio‐protection mechanisms in mammalian hibernators.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Integrated transcriptomics and metabolomics reveal protective effects on heart of hibernating Daurian ground squirrels

Yang,

Hao,

et al. 2023

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…2016; Tang et al . 2021). SOD functions to catalyze the dismutation of superoxide anions O 2 •− into dioxygen and H 2 O 2 (Wang et al .…”

Section: Methodsmentioning

confidence: 99%

Antioxidant response to severe hypoxia in Brandt's vole Lasiopodomys brandtii

et al. 2021

View full text Add to dashboard Cite

The antioxidant defense system is essential for animals to cope with homeostasis disruption and overcome oxidative stress caused by adverse environmental conditions such as hypoxia. However, our understanding of how this system works in subterranean rodents remains limited. In this study, Brandt's vole Lasiopodomys brandtii was exposed to normoxia (21% O2) or hypoxia (mild or severe hypoxia: 10% or 5% O2) for 6 h. Changes in key enzymes of the classic enzymatic antioxidant system at both mRNA and enzyme activity levels, and tissue antioxidant levels of the low‐molecular‐weight antioxidant system were determined in brain, liver, and kidney. Transcript levels of the upstream regulator NF‐E2‐related factor 2 (Nrf2) were also measured. We found that the mRNA expression of Nrf2 and its downstream antioxidant enzyme genes in L. brandtii were relatively conserved in response to hypoxia in most tissues and genes tested, except in the liver. Hepatic Nrf2, Cu/Zn SOD, GPx1, and GPx3 levels were significantly upregulated in response to mild hypoxia, whereas Mn SOD level decreased significantly in severe hypoxia. Unmatched with changes at the RNA level, constitutively high and relatively stable antioxidant enzyme activities were maintained throughout. For the low‐molecular‐weight antioxidant system, an abrupt increase of cerebral ascorbic acid (AA) levels in hypoxia indicated a tissue‐specific antioxidant response. Although hypoxia did not cause significant oxidative damage in most tissues tested, the significant decrease in antioxidant enzyme activities (GPX and GR) and increase in lipid peroxidation in the kidney suggest that prolonged hypoxia may pose a critical threat to this species.

show abstract

“…Anoksi toleranslı kaplumbağalar ve kış uykusundaki memeliler gibi hareket kapasitesi ve oksijene erişimi daha fazla olan hayvanların yapısal antioksidan savunmalarının ve mevsimsel değişken antioksidan kapasitelerinin yüksek olduğu belirtilmiştir (Lima & Savin, 2002;Storey & Storey, 2017). Gelgit bölgelerindeki yumuşakçalar ve böcekler de dahil birçok donma toleranslı türün incelenmesi sonucu antioksidan savunmanın başarılı bir donma toleransında önemli faktörlerden biri olduğu gösterilmiştir (Storey & Storey, 2017;Tang et al, 2021).…”

Section: Antioksidanlarunclassified

Hayvanlarda Soğuğa Dayanıklılık: Çift Yaşarların Kriyobiyolojisi

Yoldaş

Erişmiş

2022

Comm. J. Biol.

View full text Add to dashboard Cite

Organizmalar yaşamlarını devam ettirebilmek için abiyotik çevresel koşullara uyum sağlarlar. Özellikle ortam sıcaklığındaki değişimler; canlıların beslenme, üreme, gelişim ve morfolojileri üzerinde etkilidir. Sıra dışı sıcaklık değişimleri özellikle ektotermik hayvanlar için ölümcül olabilir. Karasal ektotermler. doğada donma noktasının altındaki sıcaklıklarda hayatta kalabilmek için davranışsal, fizyolojik ve biyokimyasal bazı özel stratejiler geliştirmişlerdir. Bazı türler göç ederek su ya da toprak altında kış uykusuna yatmak suretiyle dondurucu sıcaklıklardan kaçınırlar. Bazıları ise donma koşullarına maruz kalarak kışı geçirmek zorundadırlar. Genel olarak dondurucu soğuğa dayanıklılık donmadan kaçınma (süper soğuma) ve donma toleransı stratejilerine bağlıdır. Donmadan kaçınma durumunda vücut sıvılarının donma noktasının altındaki sıcaklıklarda sıvı formu korunurken donma toleransı stratejisini kullanan canlılarda ise vücutlarındaki toplam suyun %50’sinden fazlasının donması tolere edilebilir. Karasal hibernatör hayvanlardan bazı amfibi ve sürüngen gruplarında da tespit edilen donma toleransı stratejisi onların dondurucu kış koşullarında hayatta kalmalarını sağlamaktadır. Bu özel türler kriyoprotektif mekanizmaları ile donmanın ölümcül etkilerinden korunurlar. Donma süresince yaşamsal faaliyetleri tamamen duran bu hayvanlar çözündükten sonra kısa bir süre içerisinde de normal yaşama dönerler. Bu mucizevi mekanizmanın araştırılması yalnızca hayvanların karmaşık adaptasyonunu açıklamakla kalmaz, aynı zamanda doku ve hücre kriyoprezervasyon teknolojisine de kaynak sağlar. Bu derleme amfibilerin donma toleransı stratejilerine dair bilgiler sunarak henüz yeterince çalışılmamış bu konuda araştırma yapmak isteyenlere katkı sağlayacaktır.

show abstract

Antioxidant defense response during hibernation and arousal in Chinese soft-shelled turtle Pelodiscus sinensis juveniles

Cited by 14 publications

References 34 publications

Integrated transcriptomics and metabolomics reveal protective effects on heart of hibernating Daurian ground squirrels

Integrated transcriptomics and metabolomics reveal protective effects on heart of hibernating Daurian ground squirrels

Antioxidant response to severe hypoxia in Brandt's vole Lasiopodomys brandtii

Hayvanlarda Soğuğa Dayanıklılık: Çift Yaşarların Kriyobiyolojisi

Contact Info

Product

Resources

About