Antioxidant effect of haptoglobin phenotypes against DNA damage induced by hydrogen peroxide in human leukocytes

Moreira, Leonora R.S.; Miranda-Vilela, Ana Luisa; Silva, I.C.R.; Akimoto, Arthur K.; Klautau-Guimarães, M. N.; Grisólia, César Koppe

doi:10.4238/vol8-1gmr569

Cited by 13 publications

(7 citation statements)

References 26 publications

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“…This is because it was the only genotype where the two H 2 O 2 treated groups were not significantly more damaged than the controls. Two previous studies by our group corroborate this suggestion [ 33 , 38 ]. The first study, run with a young population group of Brasilia, indicated that among Hp* 1 alleles, Hp* 1F was the most protective, besides showing that homozygous Hp 1F-1F incurred less DNA damage than other genotypes [ 38 ].…”

Section: Discussionsupporting

confidence: 86%

“…Two previous studies by our group corroborate this suggestion [ 33 , 38 ]. The first study, run with a young population group of Brasilia, indicated that among Hp* 1 alleles, Hp* 1F was the most protective, besides showing that homozygous Hp 1F-1F incurred less DNA damage than other genotypes [ 38 ]. In the second study, carried out with runners, one of the main factors that contributed to deviation of the Hp locus from HWE was the higher frequencies of Hp1F-1F than the expected [ 33 ], indicating that this genotype can be more protective in situations that impose oxidative stress.…”

Section: Discussionsupporting

confidence: 86%

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Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

et al. 2010

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BackgroundNormal cellular metabolism is well established as the source of endogenous reactive oxygen species which account for the background levels of oxidative DNA damage detected in normal tissue. Hydrogen peroxide imposes an oxidative stress condition on cells that can result in DNA damage, leading to mutagenesis and cell death. Several potentially significant genetic variants related to oxidative stress have already been identified, and angiotensin I-converting enzyme (ACE) inhibitors have been reported as possible antioxidant agents that can reduce vascular oxidative stress in cardiovascular events.MethodsWe investigate the influences of haptoglobin, manganese superoxide dismutase (MnSOD Val9Ala), catalase (CAT -21A/T), glutathione peroxidase 1 (GPx-1 Pro198Leu), ACE (I/D) and gluthatione S-transferases GSTM1 and GSTT1 gene polymorphisms against DNA damage and oxidative stress. These were induced by exposing leukocytes from peripheral blood of healthy humans (N = 135) to hydrogen peroxide (H2O2), and the effects were tested by comet assay. Blood samples were submitted to genotyping and comet assay (before and after treatment with H2O2 at 250 μM and 1 mM).ResultsAfter treatment with H2O2 at 250 μM, the GPx-1 polymorphism significantly influenced results of comet assay and a possible association of the Pro/Leu genotype with higher DNA damage was found. The highest or lowest DNA damage also depended on interaction between GPX-1/ACE and Hp/GSTM1T1 polymorphisms when hydrogen peroxide treatment increased oxidative stress.ConclusionsThe GPx-1 polymorphism and the interactions between GPX-1/ACE and Hp/GSTM1T1 can be determining factors for DNA oxidation provoked by hydrogen peroxide, and thus for higher susceptibility to or protection against oxidative stress suffered by healthy individuals.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 86%

Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

et al. 2010

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“…As a result of its Hb‐scavenging function, Hp plays a key role in the iron metabolism and has an antioxidative capacity, preventing the iron‐induced formation of free radicals 17 . The formation of Hb–Hp complexes results in a rapid clearance of Hb from the plasma 18 . Most Hp is produced by the liver, although the skin, lungs, kidney and adipose tissue are additional sources of Hp production 19…”

Section: Introductionmentioning

confidence: 99%

“…17 The formation of Hb-Hp complexes results in a rapid clearance of Hb from the plasma. 18 Most Hp is produced by the liver, although the skin, lungs, kidney and adipose tissue are additional sources of Hp production. 19 The Hp gene is characterized by a genetic polymorphism leading to three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2.…”

Section: Introductionmentioning

confidence: 99%

The haptoglobin phenotype influences the risk of cutaneous squamous cell carcinoma in kidney transplant patients

Speeckaert

Brochez

Lambert

et al. 2011

Acad Dermatol Venereol

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“… 11 Haptoglobin also acts as a potent antioxidant, oxidative damage to DNA induced by hydrogen peroxide have been investigated among the three Hp phenotypes and finding revealed that Hp1-1 has least DNA damage compared to Hp2-1 and Hp2-2. 12 …”

Section: Introductionmentioning

confidence: 99%

Haptoglobin phenotypes and susceptibility of Schistosoma parasites infection in central Sudan

Yousif

Elagib²

2017

Mediterr J Hematol Infect Dis

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Haptoglobin (Hp) is an acute phase protein that binds the free hemoglobin (Hb), thus preventing iron loss and renal damage. Hp also has antioxidative and immunomodulatory properties. Three Hp phenotypes have been identified in human: Hp1-1, Hp2-1, and Hp2-2. Hp polymorphisms have been related to susceptibility of various diseases. In this study, we aimed to assess the possible association of Hp phenotypes polymorphism to Schistosoma parasites infection in central Sudan. We have investigated the Hp phenotypes polymorphism distribution in the serum of 125 (93 S. mansoni, 13 S. haematobium and 19 infected with both “co-infection”) parasitologically confirmed infected individuals and 208 healthy individuals served as control. Hp phenotypes have been determined by polyacrylamide gel electrophoresis followed by benzidine staining. Our study revealed that Hp1-1 percentage frequency was significantly higher in infected individuals than healthy control individuals 51% and 26% respectively. Our data suggest that Hp1-1 phenotype may upsurge the susceptibility to Schistosoma parasites infection in central Sudan.

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Antioxidant effect of haptoglobin phenotypes against DNA damage induced by hydrogen peroxide in human leukocytes

Cited by 13 publications

References 26 publications

Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

The haptoglobin phenotype influences the risk of cutaneous squamous cell carcinoma in kidney transplant patients

Haptoglobin phenotypes and susceptibility of Schistosoma parasites infection in central Sudan

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