Antioxidant effect of human adult adipose-derived stromal stem cells in alveolar epithelial cells undergoing stretch

Peñuelas, Óscar; Sánchez, Carolina; Sánchez, I.M. Méndez; Quinn, Kieran L.; Ferruelo, Antonio; Pérez‐Vizcaíno, Francisco; Esteban, Andrés; Navajas, Daniel; Nin, Nicolás; Lorente, José A.; Farré, Ramón

doi:10.1016/j.resp.2013.04.007

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…By using a bioprinted mold in pluronic to cast the hydrogels before gelation, samples with very similar geometries were obtained, thereby reducing the variability and artifacts in the experiments. Differences in the macromechanics were quantified by the stress and elastic modulus at 20% strain, which is a strain with physiological relevance in biological application such those involving cardiac 61 and respiratory 62 diseases. The values obtained for pure collagen strips were in agreement with previously reported data.…”

Section: Discussionmentioning

confidence: 99%

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Sanz-Fraile

Amoros

Mendizábal

et al. 2020

Tissue Engineering Part A

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Type I collagen hydrogels are of high interest in tissue engineering. With the evolution of 3D bioprinting technologies, a high number of collagen-based scaffolds have been reported for the development of 3D cell cultures. A recent proposal was to mix collagen with silk fibroin derived from Bombyx mori silkworm. Nevertheless, due to the difficulties in the preparation and the characteristics of the protein, several problems such as phase separation and collagen denaturation appear during the procedure. Therefore, the common solution is to diminish the concentration of collagen although in that way the most biologically relevant component is reduced. In this study, we present a new, simple, and effective method to develop a collagen-silk hybrid hydrogel with high collagen concentration and with increased stiffness approaching that of natural tissues, which could be of high interest for the development of cardiac patches for myocardial regeneration and for preconditioning of mesenchymal stem cells (MSCs) to improve their therapeutic potential. Sericin in the silk was preserved by using a physical solubilizing procedure that results in a preserved fibrous structure of type I collagen, as shown by ultrastructural imaging. The macro-and micromechanical properties of the hybrid hydrogels measured by tensile stretch and atomic force microscopy, respectively, showed a more than twofold stiffening than the collagen-only hydrogels. Rheological measurements showed improved printability properties for the developed biomaterial. The suitability of the hydrogels for 3D cell culture was assessed by 3D bioprinting bone marrow-derived MSCs cultured within the scaffolds. The result was a biomaterial with improved printability characteristics that better resembled the mechanical properties of natural soft tissues while preserving biocompatibility owing to the high concentration of collagen.

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Section: Discussionmentioning

confidence: 99%

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Sanz-Fraile

Amoros

Mendizábal

et al. 2020

Tissue Engineering Part A

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“…Replenishing of GSH may be explained in the light of the finding that BM‐MSCs infusion improved GSH homeostasis probably via increased efflux of cysteine and GSH from tissues, and/or increased GSH synthesis and recycling . Antioxidant effects of stem cells were formerly demonstrated in various lung injury models . Secretion of soluble growth factors such as keratinocyte and fibroblast growth factor as well as direct interaction with host cells probably contributes to these effects .…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Alleviate Methotrexate-Induced Pulmonary Fibrosis in Rat: Comparison with Dexamethasone

Fikry

Safar

Hasan

et al. 2015

J Biochem Mol Toxicol

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The present study examined the therapeutic effects of bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived mesenchymal stem cells (AD-MSCs) in methotrexate (MTX)-induced pulmonary fibrosis in rats as compared with dexamethasone (Dex). MTX (14 mg/kg, as a single dose/week for 2 weeks, p.o.) induced lung fibrosis as marked by elevation of relative lung weight, malondialdehyde, nitrite/nitrate, interleukin-4, transforming growth factor-β1, deposited collagen, as well as increased expression of Bax along with the reduction of reduced glutathione content and superoxide dismutase activity. These deleterious effects were antagonized after treatment either with BM-MSCs or AD-MSCs (2 × 10(6) cells/rat) 2 weeks after MTX to even a better extent than Dex (0.5 mg/kg/ for 7 days, p.o.). In conclusion, BM-MSC and AD-MSCs possessed antioxidant, antiapoptotic, as well as antifibrotic effects, which will probably introduce them as remarkable candidates for the treatment of pulmonary fibrosis.

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“…There is strong evidence that in models of ARDS, after infusion B-MSC are activated inducing secretion of multiple soluble factors that results in significantly lower levels of inflammatory cytokines in both plasma and bronchoalveolar lavage (BAL) [19,20,24,25]. B-MSC are also able to alter the systemic redox environment characteristic of ARDS to a less oxidizing value [18,26-30] and restore the alveolar epithelium and endothelium integrity and permeability, decreasing airspace neutrophils [30,31]. The transfer of functional mitochondria from B-MSC to the epithelium has been proven instrumental in the repair process of the lung [32-34] and B-MSCs have also been shown to have anti-bacterial effects that are very beneficial in the septic environment [35-38].…”

Section: Introductionmentioning

confidence: 99%

Human adult bone marrow-derived stem cells decrease severity of lipopolysaccharide-induced acute respiratory distress syndrome in sheep

et al. 2014

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IntroductionAcute respiratory distress syndrome (ARDS) is the most common cause of respiratory failure among critically ill subjects, sepsis and severe bacterial pneumonia being its most common causes. The only interventions that have proven beneficial are protective ventilation strategies and fluid conservation approaches. New therapies are needed to address this common clinical problem. Others and we have previously shown the beneficial effect of infusion of exogenous adult stem cells in different pre-clinical models of ARDS.MethodsIn the present study endotoxin was infused intravenously into 14 sheep from which 6 received different doses of adult stem cells by intrabronchial delivery to evaluate the effect of stem cell therapy.ResultsAfter administration of endotoxin, there was a rapid decline in oxygenation to hypoxemic values, indicative of severe-to-moderate ARDS. None of the animals treated with saline solution recovered to normal baseline values during the 6 hours that the animals were followed. In contrast, sheep treated with a dose of 40 million adult stem cells returned their levels of oxygen in their blood to baseline two hours after the cells were infused. Similarly, improvements in carbon dioxide (CO2) clearance, pulmonary vascular pressures and inflammation were observed and confirmed by histology and by the decrease in lung edema.ConclusionsWe concluded that instillation of adult non-hematopoietic stem cells can diminish the impact of endotoxin and accelerate recovery of oxygenation, CO2 removal and inflammation in the ovine model, making the use of adult stem cells a real alternative for future therapies for ARDS.

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Antioxidant effect of human adult adipose-derived stromal stem cells in alveolar epithelial cells undergoing stretch

Cited by 11 publications

References 44 publications

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Silk-Reinforced Collagen Hydrogels with Raised Multiscale Stiffness for Mesenchymal Cells 3D Culture

Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Alleviate Methotrexate-Induced Pulmonary Fibrosis in Rat: Comparison with Dexamethasone

Human adult bone marrow-derived stem cells decrease severity of lipopolysaccharide-induced acute respiratory distress syndrome in sheep

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