Antioxidant Effect of Polyoxidonium and Metaprot during Bronchopulmonary Inflammation in Rats

Zarubina, I. V.; Shabanov, Petr D.

doi:10.1007/s10517-015-3137-9

Cited by 4 publications

(3 citation statements)

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“…Both preparations are classified as phytohormones and are highly effective adaptogens; they act as stress protectors and have an anti-toxic effect. (13) The work with animals was carried out in accordance with the principles of humanism laid down in the directives of the European Community (86/609/ЕЕС) and the Declaration of Helsinki, in accordance with the "Animal experimentation legislations". The study was approved by the Local Ethic Committee of the S.M.…”

Section: Methodsmentioning

confidence: 99%

“…(7)(8)(9)(10)(11) Krezacin (hydroxyethylammonium methylphenoxyacetate) and Metaprot (ethylthiobenzimidazole hydrobromide monohydrate) were created in Russia in the middle of the last century and proved to have antihypoxic, energy-stabilizing (ergotropic) and immunomodulating properties. (12)(13)(14)(15)(16) However, studies of these medicines regarding bronchopulmonary diseases have not been carried out.…”

Section: Introductionmentioning

confidence: 99%

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Cytokine Profile and Its Correction by Immunomodulators in Experimental Bronchopulmonary Inflammation in Rats

Darenskaya¹,

Mokrenko²,

Shabanov³

et al. 2019

IJBM

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The aim of this study was to assess the changes in the levels of the blood serum pro-and anti-inflammatory cytokines in rats with experimental bronchopneumonia and after administration of immunomodulators. Materials and Methods: Research was carried out on mature male Wistar rats (n=111), weighing 220-250g. The bronchopulmonary inflammation in the rats was modeled by administration of 0.1 ml of gum turpentine in the rats' trachea under etherization. All rats were divided into four groups: Group 1 included intact animals (n=18); Group 2 included animals having bronchopneumonia (n=19); Group 3 included animals having bronchopneumonia treated with Krezacin (n=15); Group 4 included animals having bronchopneumonia treated with Metaprot (n=16). Right after the operation and for the next 5 days, a solution of one of the test immunomodulators was injected (once a day) abdominally in the experimental animals: 25mg/kg of Krezacin or 25mg/kg of Metaprot. On Day 5 of the experiment, the animals were decapitated under anesthesia and blood samples were collected. The concentration of cytokines in the blood was determined by the flow immunofluorometry method with the device Bio-RadLaboratories (USA). Results: The administration of Krezacin in animals with bronchopneumonia, in comparison with untreated animals, was accompanied by a statistically significant increase in the levels of IL-1β (by 3.43 times), IL-2 (by 1.95 times), INF-γ (by 3.29 times), and MCP-1 (by 2.07 times) and a decrease in the levels of TNFα (by 1.85 times) and IL-6 (by 2.20 times). Administration of Krezacin increased anti-inflammatory cytokines considerably: IL-4 by 5.22 times and IL-10 by 2.41 times. Analysis of results after administration of Metaprot demonstrates an increase in the levels of IL-1β (by 2.57 times), IL-2 (by 2 times), INF-γ (by 3.21 times), and MCP-1 (by 1.98 times) and a decrease in the levels of TNFα (by 2.17 times) and IL-6 (by 2.5 times), compared to data on untreated animals. Anti-inflammatory cytokines also increased greatly: IL-4 by 5.3 times and IL-10 by 3.71 times. Conclusion: The results obtained show that investigated preparations reduce the severity and intensity of bronchopneumonia in rats. Krezacin and Metaprot demonstrate the properties of true immunomodulators, increasing protective properties of cytokines and providing the adaptive response of the body in severe bronchopulmonary pathology.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytokine Profile and Its Correction by Immunomodulators in Experimental Bronchopulmonary Inflammation in Rats

Darenskaya¹,

Mokrenko²,

Shabanov³

et al. 2019

IJBM

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“…In clinical practice, bemithyl is used as an antiasthenic drug, when weakness, rapid fatiguability and decreased availability are observed. Aminothiol antihypoxants can be prescribed as supplementary drugs in intensive care and in urgent medicine [12]. Amtizol is assigned in common hypoxia due to blood insufficiency, critical hemodynamic disorders, asphyxia, resection of the lung.…”

Section: гипоксия и антигипоксанты в фокусе черепно-мозговая травмаmentioning

confidence: 99%

Hypoxia and antihypoxants, focus on brain injury

Shabanov

Zarubina

2019

Rev Clin Pharm Drug Ther

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Experiments on rats showed that the individual resistance of the body to acute hypoxia is of decisive importance in the early recovery period after mechanical craniocerebral trauma. Antihypoxant ethomersol administration (25 mg/kg, 3 days, intraperitoneally) following trauma decreased behavioral impairments in rats with different levels of resistance to acute hypoxia, restored the structure of individual behavior, and prevented metabolic disturbances in the brain. Monotherapy of consequences of craniocerebral trauma with antidepressant pyrazidol (1 mg/kg) produced an anxiolytic effect in animals highly resistant to hypoxia and activating effect on low resistant animals. Treatment with bemithyl, an antihypoxant of benzimidazole structure, in a dose of 25 mg/kg produced a cerebroprotective effect and normalized individual behavioral characteristics, parameters of energy metabolism, and state of the antioxidant system in the brain of highly and low resistant rats. The effect of bemithyl was most pronounced in highly resistant animals. During combined treatment, pyrazidol and bemithyl had an additive effect in animals of both groups. They normalized behavioral reactions and prevented the development of metabolic disturbances in the brain.

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