Antioxidant effects of calligonum extract on ovarian tissue of PCO model: An experimental study

Tahmasebi, Fatemeh; Movahedin, Mansoureh; Mazaheri, Zohreh

doi:10.29252/ijrm.16.10.641

Cited by 11 publications

(10 citation statements)

References 23 publications

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“…27 The protecting effect of quercetin on oxidative stress injury was studied by Yuling Mi Caiqiao Calligonum could significantly increase the TAC of ovarian tissues as well as the percentage of in vitro fertilization compared to the PCO group. 13 The relationship between oxidative stress and antioxidant was analyzed using the correlation test. In living organisms, there is a balance between the level of oxidants and antioxidants in cells.…”

Section: Discussionmentioning

confidence: 99%

“…10 Overall, 12 phenolic acids and 12 flavonoids were observed in the Calligonum comosum extract, 11 which chiefly contained flavonols (quercetin) and Flavanols (epicatechin and catechin (+)). 12 The Calligonum comosum is often used in traditional medicine to treat several diseases such as polycystic ovary, 13 hepatotoxicities, 14 diabetes 15 and osteoporosis. 16 Because of its antioxidant capacities, Calligonum extract is used as anti-ulcer 17 and anti-inflammatory drug.…”

Section: Introductionmentioning

confidence: 99%

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The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

Barati¹,

Movahedin²

2021

Pharm Sci

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Background: Spermatogenesis is a programmed route of germ cell proliferation and differentiation that could produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing ROS production in cells, therefore plants extract administration with antioxidant property can prevent of cell damage. In the present study, antioxidant effects of Calligonum extract on proliferation and colonization rate of spermatogonial cells was assessed. Materials and Methods: After isolation and culture of spermatogonial stem cells (SSCs) from neonatal mice (4-5 day old) and identification by PLZF and Oct4 markers, the therapeutic effect of Calligonum comosum extract was measured on the cells treated with H2O2. Induced oxidative stress cells were treated with 10 μg/ml extract for 3 weeks. ROS levels were assessed by flow cytometry, proliferation by cell count and TAC by FRAP assay. Also, the apoptosis rate was measured with P53 and Bax genes by real time PCR method. Results: After three weeks of treatment, the Calligonum group showed significantly lower ROS level relative to the H2O2 group. Antioxidants levels were in Calligonum group significantly higher than the H2O2 group (P≤0.05). There was a strong inverse relationship between the two groups. Proliferation and colonization rate were in Calligonum + H2O2 group significantly higher than H2O2 group alone (P≤0.05). Finally, the results of the study indicated that P53 and Bax expression decreased in Calligonum + H2O2 group compared to H2O2 group. Conclusion: The results of present study expressed that Calligonum as a plant with antioxidant effect could reduce the level ofROS, and increase proliferation and colonization rate and TAC. In other hand, 30 μM doses of H2O2increased oxidative stress and apoptosiswhile decreased proliferation of SSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

Barati¹,

Movahedin²

2021

Pharm Sci

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“…¨Journal of Environmental Biology, January 2022( Tahmasebi et al, 2018). In view of the above, this study was conducted to evaluate the potential protective effects of mangosteen peel extract against BPA-induced abnormalities onpregnant mice fetus at implantation stage and offspring at postparturition.…”

Section: O N L I N E C O P Ymentioning

confidence: 99%

“…Escalation of ROS can induce pathological consequences in oocyte maturation, ovulation, fertilization, implantation, and embryo development, which can ultimately influence the outcome of pregnancy (Wang et al, 2017). Previous studies have reported that antioxidants play significant role in treating reproductive disease and infertility by controlling oxidative stress (Agarwal et al, 2012;Liu et al, 2018;Tahmasebi et al, 2018;Wang et al, 2017). Antioxidant substances especially quercetin, catechin and anthocyanins have been proven its potential to ameliorate polycystic ovary syndromes (PCOS) and prevent ovarian aging due to their antioxidant effects on ROS pathways by either inhibiting NF-κB pathway or by increasing antioxidase activities and their gene expression (Liu et al, 2018;Tahmasebi et al, 2018).Current research trends are being focused on exploring natural plant-based source of antioxidants to counter the effects of oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported that antioxidants play significant role in treating reproductive disease and infertility by controlling oxidative stress (Agarwal et al, 2012;Liu et al, 2018;Tahmasebi et al, 2018;Wang et al, 2017). Antioxidant substances especially quercetin, catechin and anthocyanins have been proven its potential to ameliorate polycystic ovary syndromes (PCOS) and prevent ovarian aging due to their antioxidant effects on ROS pathways by either inhibiting NF-κB pathway or by increasing antioxidase activities and their gene expression (Liu et al, 2018;Tahmasebi et al, 2018).Current research trends are being focused on exploring natural plant-based source of antioxidants to counter the effects of oxidative stress. The mangosteen peel extract (MPE) is an industrial waste but contain natural antioxidants such as phenolic acids and flavonoids, which possess biological and medicinal properties, especially antioxidant properties (Suttirak and Manurakchinakorn, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Effect of mangosteen peel extract on BPA-exposed murine during gestation

Loh¹,

Kwong²,

Chan³

et al. 2022

JEB

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Aim: To evaluate the potential protective effects of mangosteen peel extract against BPA-induced abnormalities on-pregnant mice fetus at implantation stage and offspring at post-parturition. Methodology: Pregnant mice were orally administered with BPA (100mg kg-1 b.wt.) and mangosteen peel extract (200mg kg-1 b.wt.) for 16 days. In order to evaluate the effect of MPE treatment on fetus at implantation stage, the pregnant mice were euthanized at day 18 and the fetus number and morphology were examined. Another group of treated dams, were allowed to undergo parturition for evaluating the of maternal weight, litter size and offspring sex-skewness. Results: Upon feeding Mangosteen peel extract (MPE), the average daily weight gain of dams were not significantly different from the control and BPA treated dams. The fetus derived from BPA treated dams were detected with abnormalities such as under development, haemorrhage and absence of vein, whereas fetus from dam treated with MPE and BPA as well as control were normal. The average litter size of all the treatment groups were not significantly different from the control group. BPA treated mice had lower pups survival up to 6 weeks compared to the groups treated with MPE and control. Test of proportion analysis showed BPA-treated group had significantly higher fraction female ratio. Interpretation: BPA is known as endocrine disruptor causing oxidative stress to female reproductive system, hence mangosteen peel extract contains antioxidant substances that have the potential to ameliorate the adverse effects of BPA exposure on dams during pregnancy and its fetus development.

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