Antioxidant Effects of Some Drugs on Immobilization Stress Combined with Cold Restraint Stress

Popović, Mira; Janićijević-Hudomal, Snežana; Kaurinović, Biljana; Rašić, Julijana; Trivić, Svetlana; Vojnović, Matilda

doi:10.3390/molecules14114505

Cited by 26 publications

(18 citation statements)

References 38 publications

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“…Şahin and Gümüşlü (2007) found CAT activities increased in the brain, liver, and heart. Popovic et al (2009) also found increased cerebral activity of CAT in immobilization and cold-induced stress. Kaushik and Kaur (2003) reported that CAT activity was decreased in the heart and liver due to cold stress.…”

Section: Discussionmentioning

confidence: 87%

Melatonin is effective in reducing stress-induced organ damage in Wistar albino rats

Eşrefoğlu¹,

Akıncı²,

Elbe³

et al. 2014

Turk J Biol

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In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, the brain, cerebellum, heart, and liver were rapidly removed. The main histopathological damage scores (MHDSs) of the stress and stress + saline groups were higher than those of control group for all of the organs. The MHDSs of melatonin-administered group were lower than those of stress and stress + saline groups. The main tissue superoxide dismutase activities of the stress + melatonin group were even higher than those of the control group in the cerebellum and liver, and main tissue catalase activities of the stress + melatonin group were even higher than those of control group in all of the organs. As a conclusion, we found melatonin very effective in reducing stress-induced organ damage by inhibiting lipid peroxidation and supporting the cellular antioxidant defense system.

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Section: Discussionmentioning

confidence: 87%

Melatonin is effective in reducing stress-induced organ damage in Wistar albino rats

Eşrefoğlu¹,

Akıncı²,

Elbe³

et al. 2014

Turk J Biol

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“…Tramadol is one of the synthetic opioids that has toxic effects at the cellular level by increasing LPO that can be used as a marker of the ROS-induced cell damages [48]. Moreover, it has been found that LPO is significantly increased in chronic heroin users [49].…”

Section: Discussionmentioning

confidence: 99%

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Ibrahim

Salah-Eldin

2019

Pharmacology

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Aim: The present study aimed to elucidate the effects of tramadol on the testicular functions of adult male rats due to the chronic usage of tramadol and the effect of its withdrawal. Method: Adult male albino rats were classified into the following 3 groups: (I) a control administered with normal saline and (II) tramadol-treated rats (40 mg/kg b.w. orally) for 21 successive days; and (III) like the rats in the second group but kept for 4 weeks after the last tramadol dose to study the effect of tramadol withdrawal. At the end of the experimental period, blood was collected and specimens from testis were taken for histopathological, biochemical, and molecular studies. A reverse transcription-polymerized chain reaction after RNA extraction from specimens was detected for the anti-apoptotic and pro-apoptotic genes in testicular tissues. Also, malondialdehyde (MDA) was measured in tissues homogenate and antioxidant enzymes activities were evaluated. Results: The results of this study demonstrated histological changes in testicular tissues in groups II and III compared to the control group, accompanied with increased apoptotic index and proved by increased B-cell lymphoma-2 (Bcl-2) associated-X-protein and caspase-3 expression, whereas anti-apoptotic Bcl-2 markedly decreased. Moreover, in tramadol-abused and -withdrawal groups, the MDA level increased, while the antioxidant enzymes activity decreased and revealed oxidative stress, indicating that tramadol is harmful at the cellular level and can induce apoptotic changes in testicular tissues. The withdrawal effect showed signs of improvement, but it did not return to normal levels. Conclusions: It could be concluded that the administration of tramadol causes abnormalities on testicular tissues associated with oxidative stress, which confirmed the risk of increased oxidative stress on testicular tissues due to tramadol abuse.

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“…The rats were housed 5 per cage under constant environmental conditions (20-24°C; 12 h light/dark cycle), and were given ad libitum access to standard pelleted food and water. After the administration of quail egg yolk and albumen extracts for 21 days, combined IS and cold-restraint stress (CRS) test was performed by immobilizing animals in the cold chamber at 4 ± 0.3°C; the plexiglass cage volume was adjusted to the size of the animal, to restrain completely their movements (Popovic et al 2009) for 2 h, except group 1. Group 2 animals were treated after stress with diazepam (5 and 10 mg/mL BW).…”

Section: Experimental Designmentioning

confidence: 99%

“…Cold and immobilization stressors can generate oxidative stress as well as skeletal muscle fatigue. Cold immobilization stress also called immobilization stress and cold-restraint stress had been described by Popovic et al (2009) as experimental induction of very extreme condition which cannot be carried out on human, but believed to have same effect on human as it revealed in animal models. Free radicals cause oxidative degradation of lipids, proteins, nucleic acids, and carbohydrates molecules thereby compromising cell integrity and function.…”

Section: Introductionmentioning

confidence: 99%

BioActivities of Coturnix japonica (quail) egg yolk and albumen against physiological stress

Oladipo

Ibukun

2016

Food Science & Nutrition

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Cold and immobilization stressors can generate oxidative stress as well as skeletal muscle fatigue. Free radicals cause oxidative degradation of lipids, proteins, nucleic acids, and carbohydrates molecules thereby compromising cell integrity and function. Coturnix japonica (quail) egg had been described as being very functional biochemically, due to the essential biomolecules it contains in very regulated quantity. This study was designed to evaluate the in vitro antioxidant activity of extracts of quail egg yolk and the albumen. The assessment of the antioxidant potentials was typified using the total antioxidant capacity, and ABTS, DPPH (1‐diphenyl‐2‐picrylhydrazyl) and hydroxyl radicals scavenging activities. Others are reducing power, metal chelating and lipid peroxidation inhibition activities. The antistress activities of quail egg yolk and albumen were evaluated on hepatopathic enzymes as well as endogenous antioxidant enzymes. The total antioxidant activities of the yolk extract (YE) and the albumen extracts (AE) were, respectively, 186.57 ± 6.441 mg/g and 172 ± 10.690 mg/g AAE (Ascorbic Acid Equivalent). The YE exhibited significant, potent and appreciable antioxidant activities than AE in a concentration‐dependent manner. The study confirmed that quail egg yolk contained highly antioxidative bioactive compounds not present in albumen, contributing to its (yolk) overall antioxidant and anti‐inflammatory (antistress) properties, thus necessitating their (albumen and yolk) beneficial effects in the management of oxidative and inflammatory conditions.

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Antioxidant Effects of Some Drugs on Immobilization Stress Combined with Cold Restraint Stress

Cited by 26 publications

References 38 publications

Melatonin is effective in reducing stress-induced organ damage in Wistar albino rats

Melatonin is effective in reducing stress-induced organ damage in Wistar albino rats

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

BioActivities of Coturnix japonica (quail) egg yolk and albumen against physiological stress

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