Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes

Carbone, Maria Cristina; Tatone, Carla; Monache, Simona Delle; Marci, Roberto; Caserta, Donatella; Colonna, Rosella; Amicarelli, Fernanda

doi:10.1093/molehr/gag090

Cited by 180 publications

(149 citation statements)

References 27 publications

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“…An increase in ROS levels, as well as a decline in autophagic markers (LC3-II) in human granulosa cells of older women suggests that there is a decline in reparative autophagy with age leading to granulosa cell apoptosis (Tatone et al 2006, Vilser et al 2010). This result is in concordance with the well-documented, age-related decline in female fertility (Carbone et al 2003, Tatone et al 2006.…”

Section: Ovaries and Testessupporting

confidence: 91%

Autophagy in the endocrine glands

Weckman¹,

Ieva²,

Rotondo³

et al. 2014

Journal of Molecular Endocrinology

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Autophagy is an important cellular process involving the degradation of intracellular components. Its regulation is complex and while there are many methods available, there is currently no single effective way of detecting and monitoring autophagy. It has several cellular functions that are conserved throughout the body, as well as a variety of different physiological roles depending on the context of its occurrence in the body. Autophagy is also involved in the pathology of a wide range of diseases. Within the endocrine system, autophagy has both its traditional conserved functions and specific functions. In the endocrine glands, autophagy plays a critical role in controlling intracellular hormone levels. In peptide-secreting cells of glands such as the pituitary gland, crinophagy, a specific form of autophagy, targets the secretory granules to control the levels of stored hormone. In steroid-secreting cells of glands such as the testes and adrenal gland, autophagy targets the steroid-producing organelles. The dysregulation of autophagy in the endocrine glands leads to several different endocrine diseases such as diabetes and infertility. This review aims to clarify the known roles of autophagy in the physiology of the endocrine system, as well as in various endocrine diseases.

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Section: Ovaries and Testessupporting

confidence: 91%

Autophagy in the endocrine glands

Weckman¹,

Ieva²,

Rotondo³

et al. 2014

Journal of Molecular Endocrinology

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“…Furthermore, the expression of antioxidant enzymes in cells that produce steroids (granulosa, thecal and luteal cells) was also reported [42,43]. This suggests that the oocyte is physiologically exposed to oxidative stress and to antioxidant activity both present in the FF [44].…”

Section: Discussionmentioning

confidence: 82%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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“…In contrast, GSH and glutathione transferase (GST), which are effective in the removal of free radicals, are reduced in oocytes with age (88). Similarly, Carbone et al demonstrated that follicular fluid from older women exhibited a reduced level of glutathione transferase and catalase activities and a higher level of superoxide dismutase (SOD) activity (89). Taken together, the results indicated that reproductive aging is accompanied by a change in the anti-oxidant enzymatic pattern that could impair ROS scavenging efficiency in the follicular environment.…”

Section: Ovarian Agingmentioning

confidence: 84%

MECHANISMS IN ENDOCRINOLOGY: Aging and anti-aging: a Combo-Endocrinology overview

Diamanti-Kandarakis

Dattilo²,

Macut

et al. 2017

European Journal of Endocrinology

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Aging and its underlying pathophysiological background has always attracted the attention of the scientific society. Defined as the gradual, time-dependent, heterogeneous decline of physiological functions, aging is orchestrated by a plethora of molecular mechanisms, which vividly interact to alter body homeostasis. The ability of an organism to adjust to these alterations, in conjunction with the dynamic effect of various environmental stimuli across lifespan, promotes longevity, frailty or disease. Endocrine function undergoes major changes during aging, as well. Specifically, alterations in hormonal networks and concomitant hormonal deficits/excess, augmented by poor sensitivity of tissues to their action, take place. As hypothalamic-pituitary unit is the central regulator of crucial body functions, these alterations can be translated in significant clinical sequelae that can impair the quality of life and promote frailty and disease. Delineating the hormonal signaling alterations that occur across lifespan and exploring possible remedial interventions could possibly help us improve the quality of life of the elderly and promote longevity.

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Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes

Cited by 180 publications

References 27 publications

Autophagy in the endocrine glands

Autophagy in the endocrine glands

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

MECHANISMS IN ENDOCRINOLOGY: Aging and anti-aging: a Combo-Endocrinology overview

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