Antioxidant Enzymes in Barley Green Biomass

Abstract

Green biomass of young barley plants exhibited statistically significant higher activity of superoxide dismutase (SOD) and catalase (CAT) at sampling I (in the phase of plant development DC 29) compared to the later sampling II (DC 31). Significant effects of varieties, years and interactions of the studied factors on the activity of the studied antioxidants were determined. During the experiment period (2005-2007), the variety Sebastian provided statistically significant higher average SOD activity (486 U.g-1) versus the variety Malz (416 U.g-1 dry matter) and line KM1910 (418 U.g-1 dry matter). No statistically significant difference was recorded between the latter two varieties. Average catalase activity of the varieties did not show any significant difference. Significantly higher CAT activity in the sampling I was recorded on average of years and locations in the variety Sebastian and hull-less line KM1910 (935 and 907 U.g-1) compared to the variety Malz (675 U.g-1). We can state that green biomass of young spring barley plants taken during the growth phase DC 29 was a significant source of enzymes catalase and superoxide dismutase in the course of the experiment (2005-2007).

The content of vitamin E in hop cones of the Saaz variety

“…This is in good accordance with the analysis of barley green mass; there were also only tocopherols determined (Ehrenbergerová et al, 2009).…”

Antioxidant activity of superoxide dismutase in spring barley grain.

“…Březinová Belcredi et al [16] did not find any significant difference in SOD activity among barley varieties grown in three localities. Ehrenbergerová et al [17] also studied activity in green biomass of varieties/lines of spring barley in the localities Žabčice (518 U.g -1 ) and Kroměříž (363 U.g -1 ), and they determined a significant difference between these localities in the average activity of the studied set.…”

Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgareL.) under controlled severe drought

“…This is because ROS accumulation and the upregulation of antioxidant enzymes are dependent on plant species, plant genotype, stress severity, stress duration, plant development, and metabolism (Reddy et al 2004). Indeed, a study on barley plants under normal growth conditions showed that SOD and CAT activities were higher at the early developmental stage compared to the late developmental stage (Ehrenbergerová et al 2009). …”