Antioxidant- Influenced Alterations in Glutathione Reductase Activity in Different Age Groups of Male Mice

Khanna, S. N.; Garg, Sanjay; Sharma, Suraj

doi:10.1159/000213301

Cited by 9 publications

(5 citation statements)

References 6 publications

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“…The increase in enzyme activty during reproductive phase could be due to increased metabolic rate during this phase of life resulting in increased production of free radicals and peroxides which need detoxification. These results corroborate with Khanna et al [36] who found an increase in GR activity during reproductive phase in male mice. A gradual decline with age was observed in GR activity of both the sexes.…”

Section: Vol 40 No 4 1996supporting

confidence: 93%

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Gender specific alterations in antioxidant status of aging Zaprionus paravittiger fed on propyl gallate

1996

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Zaprionus paravittiger fed on propyl gallate (PG) supplemented diet (2.5, 25 and 250 µg/ml) showed an increase in life span. Further increase in concentration (2500, 5000 and 7500 µg/ml) accelerated the mortality rate. Females exhibited longer life span as compared to males. Antioxidant enzymes (catalase, peroxidase and glutathione reductase) were measured in control and optimum concentration of PG (25 µg/ml) fed flies at various age intervals. Antioxidant enzyme activities showed an increase during reproductive phase. Catalase and glutathione reductase activities decreased with age however no significant change was observed in peroxidase activity. The females exhibited higher enzyme activities as compared to males in control and PG fed group at most of the age intervals. PG feeding caused a significant increase in catalase and glutathione reductase activities in both the sexes. These findings suggest the PG has dose dependent and sex specific influence on longevity of Z. paravittiger and support the view that longevity and activity of antioxidant enzymes are positively linked.

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Section: Vol 40 No 4 1996supporting

confidence: 93%

“…The flies were homogenized in suitable buffer (as described in each assay) using Polytron homogenizer at 4"C for 30 sec (2x15 sec with 15 sec cooling interval). Estimations of antioxidative enzymes (catalase, peroxidase and glutathione reductase) were done on whole body homogenates of males and females at 1,8,15,22,29,36 and 43 days of age.…”

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confidence: 99%

Gender specific alterations in antioxidant status of aging Zaprionus paravittiger fed on propyl gallate

1996

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“…The induction of GR has been demonstrated with other antioxidants such as butylated hydroxyanisole, propyl gallate, ·-tocopherol [6], lobenzarit (disodium 4-chloro-2,2-iminodibenzoate) [24], and fraxetin (6-methoxy-7,8-dihydroxycoumarin) [25].…”

Section: Discussionmentioning

confidence: 98%

“…Based on this theory, experimental gerontologists, particularly those attempting to retard senescence by environmental manipulation, include the use of nutrients or drugs that increase antioxidants in gerontological research [5]. The enhanced activity of glutathione reductase (GR) by treatment with phenolic antioxidant compounds, such as 3,5-di-terc-butyl-4-hydroxytoluene (BHT), appears to be indicative of an enhanced cellular reducing potential for protecting the organism against oxidative damage [6]. Moreover, studies have shown that mice treated with this synthetic phenolic antioxidant under optimal living conditions have a significant increase in median life span [7].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Antioxidant (6,7-Dihydroxycoumarin) Esculetin on the Glutathione System and Lipid Peroxidation in Mice

Martín‐Aragón

Benedı́²,

Villar³

1997

Gerontology

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The attempt to retard senescence by environmental manipulation includes the use of nutrients or drugs that decrease the oxidative damage to tissues associated with aging. The effects of esculetin treatment (25 mg/kg, orally for 30 days), a phenolic antioxidant compound, on the glutathione system and lipid peroxidation were examined in liver supernatants from male C57BL/6J mice. The effects of esculetin were compared to treatment with 3,5-di-terc-butyl-4-hydroxytoluene (BHT), a well-known synthetic phenolic antioxidant. Reduced glutathione (GSH) concentration in liver supernatants was only increased significantly in esculetin-treated mice compared to control animals, whereas the concentration of oxidized glutathione (GSSG) was significantly decreased by BHT treatment compared to the control group. The GSSG/GSH ratio was significantly lower in esculetin and BHT groups than in the control group. The decrease in this ratio was greater in BHT-treated mice than in esculetin-treated mice. Increases in glutathione reductase (GR) activity were observed with both treatments, although BHT resulted in a superior induction of this activity compared to esculetin. The extent of decline in the GSSG/GSH ratio was correlated with the increase in GR activity. The formation of thiobarbituric acid-reactive substances (TBARs), an index of stress, was lower following treatment with esculetin and BHT compared to control mice (although not significant). This index was very similar for both treatments. Based on the level of TBARs obtained in this study, the accumulation of lipid peroxides declines when the GSH levels are enhanced or GSSG levels are decreased. Finally, we found similar antioxidant effects in vivo with esculetin and BHT treatments and a decrease in the oxidative damage evaluated. The enhancement of glutathione status following esculetin treatment could be a possible defense strategy for the organism under ‘stress conditions’ and may be related to the delay of age-dependent degenerative disorders.

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“…The decrease in the GSSG concentration observed in PDL 50→75 cells following WSP and HC treatments could be explained by the increase in GR activity, because the decrease in the GSSG concentration correlates with an increase in the GR activity. In fact, the induction of GR activity has been demonstrated with other antioxidants such as α-tocopherol, butylated hydroxyanisol, and propyl gallate (20). The induction of GR activity suggests that the critical factor was the maintenance of the low GSSG level (4).…”

Section: Discussionmentioning

confidence: 97%

Effects of radical scavenger protein from broad beans on glutathione status in human lung fibroblasts

Okada

Okada²

2007

Environ Health Prev Med

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Antioxidant- Influenced Alterations in Glutathione Reductase Activity in Different Age Groups of Male Mice

Cited by 9 publications

References 6 publications

Gender specific alterations in antioxidant status of aging Zaprionus paravittiger fed on propyl gallate

Gender specific alterations in antioxidant status of aging Zaprionus paravittiger fed on propyl gallate

Effects of the Antioxidant (6,7-Dihydroxycoumarin) Esculetin on the Glutathione System and Lipid Peroxidation in Mice

Effects of radical scavenger protein from broad beans on glutathione status in human lung fibroblasts

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