Antioxidant Mechanism is Involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in Rats

Abstract

This research was performed in order to determine the potential protective effects of ozonized sunflower oil (OSO) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species (ROS), lipid peroxidation, and some important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in these effects. OSO was administered to rats intragastrically by a cannula and it was applied during four days to animals. The doses of OSO administered daily to each group of rats were 4, 12, and 24 mg/kg, respectively, and one hour after the last treatment, absolute ethanol (1 mL/200 mg body weight) was administered. Our results showed that gastric ulcer index was significantly reduced in rats pretreated with OSO as compared with ethanol-treated controls. However, in rats pretreated with OSO, no significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD and GSH-Px activities were significantly increased in gastric mucosa of OSO-pretreated rats with respect to those treated with ethanol alone. In summary, our results demonstrate that OSO pretreatment exerts protective effects in ethanol-induced gastric ulcers in rats. Furthermore, these results provide evidence that these protective effects of OSO are mediated at least partially by stimulation of some important antioxidant enzymes such as SOD and GSH-Px, which are scavengers of ROS and therefore prevent gastric injury induced by them.

Protective Effect ofAmphipterygium adstringensExtract on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice

“…CAT activity was slightly higher in the AaEE + DSS group than in the DSS group, although the difference was not significant; it has been reported that there is an increase in CAT activity by administering natural products as treatments against UC and this appears to be due to the fact that CAT has a lower affinity for that ROS comparing to SOD and GPx. This result comes in concordance with many previous studies that reported that GPx plays a much greater role in the removal of H 2 O 2 than CAT [29–31]. Therefore, our findings indicate that AaEE treatment of colitis might be reducing the extent of colonic injury by its antioxidant ability.…”

Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status

“…These processes detoxify endogenous toxic materials such as peroxidized lipids. GPx is an enzyme with peroxidase activity, which reduces lipid hydroperoxides and free hydrogen peroxide to water [26]. GR is an important antioxidant enzyme that reduces glutathione disulfide to the sulfhydryl form of GSH [32].…”

Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat

“…Currently, there is consensus that the deleterious effects of ethanol on gastric mucosa are consequence of the enhanced lipid peroxidation and decreased glutathione levels [37]. On the other hand, in ischemia-reperfusion model, the injuries occur without the use of chemical agents [38].…”