Antioxidant Potentials of Flaxseed by in Vivo Model

Abstract

The present study reports the antioxidant activity of flaxseed as measured by feeding weanling albino rats with 5.0% and 10.0% of flaxseed (constituting approximately 0.75 and 1.5 g kg(-)(1)) for 14 days followed by challenging animals with 2.0 g kg(-)(1) b.w. CCl(4) as toxin. Activity was assessed by measuring hepatic marker enzymes like catalase, superoxide dismutase (SOD), and peroxidase and comparing with those from the normal group and from a group receiving toxin without flaxseed. Treatment of CCl(4) at dose of 2.0 g kg(-)(1) b.w. decreased the activities of various antioxidant enzymes such as catalase, superoxide dismutase (SOD), and peroxidase by 35.6%, 47.76%, and 53.0%, respectively, compared to the control group, and the lipid peroxidation value increased nearly 1.2-fold compared to that of the group treated with toxin without flaxseed. Pretreatment of rats with 5.0% flaxseed followed by CCl(4) treatment caused restoration of catalase, SOD, and peroxidase by 39.7%, 181.42%, and 123.7%, respectively, as compared to control. The group treated with 10.0% flaxseed has shown the restoration of 95.02%, 182.31%, and 136.0% of catalase, SOD, and peroxidase. In the case of the group treated with toxin without flaxseed, the level of superoxide dismutase and the catalse value decreased 91.4% and 55.33%, respectively, in comparison with the control group. These results clearly indicate the beneficial effect of flaxseed components as an antioxidant as seen by restoration of hepatic enzymes, which were varied from normal to one due to toxicity induced by toxin (CCl(4)). Owing to this property, the flaxseed known for its functional properties can be further extended to exploit its possible application for various health benefits as nutraceuticals and food ingredient.

Nutritional sub-fertility in the dairy cow: towards improved reproductive management through a better biological understanding

“…Flaxseed hulls are rich in plant lignans, which are recognised as strong antioxidants (Prasad, 2000). They enhance endogenous level response by upregulating the expression of genes encoding the enzymes such as superoxide dismutase, catalase and peroxidase (Rajesha et al, 2006). Our recent results (Petit et al, 2009) have also shown that flaxseed hulls increase the activity of the enzyme superoxide dismutase in the mammary gland that is responsible for the removal of free radicals leading to oxidative stress.…”

Secoisolariciresinol Diglucoside - A Phytoestrogen Nutraceutical of Flaxseed: Synthesis and Evaluation of Antioxidant Potency

“…[8,9] SDG, via various in vivo and in vitro studies was found to be a potential antioxidant agent. [10][11][12] It also appears to be effective against breast and colon cancer, [13] hyper cholesterolemic atherosclerosis, diabetes, [14] lupus nephritis, immune and inflammatory reactions. [15,16] In addition, SGD is found to possess a good antibacterial activity against gram positive and negative bacterial species.…”

A simplified and small-scale sample preparation technique for determining astaxanthin, canthaxanthin, and β-apo-8′-carotenoic acid ethyl ester in hen’s egg yolk

“…In comparison to the previous techniques for determining xanthophylls in egg yolk samples [18][19][20], the procedure used in this study is an easy and small-scale technique that minimizes organic solvent consumption in the preparation of AX, CX, and ACAEE. The extract obtained by the extracting operation was purified by subsequent centrifugal monolithic silica spin mini-column, MonoSpin ® -SI.…”

“…Without regard for industrial nations and developing countries, the optimal harmonized method for chemical monitoring in foods must be simple, small scale, economical in time and cost, and must cause negligible harm to the environment and analysts. Several techniques based on high-performance liquid chromatography (HPLC) for the quantification of xanthophylls in egg yolk samples have been reported [18][19][20][21]. However, these methods cannot detect simultaneous AX, CX, and ACAEE; and they involve several analytical steps in the sample preparation, which are time-and cost-consuming and do not permit the determination of a large number of samples.…”