Antioxidant profile of constituents isolated from <i>Polygonatum</i><i>verticillatum</i> rhizomes

Khan, Haroon; Saeed, Muhammad; Muhammad, Naveed; Khan, Aman Nawaz; Ullah, Rooh

doi:10.1177/0748233713498454

Cited by 20 publications

(15 citation statements)

References 16 publications

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“…The fruits of C. sinensis and C. anrantifolia showed the presence of phenols, flavonoids, reducing sugars, steroids, terpinedes, and tannins, while the fruits of C. limonum indicated the presence of phenols, flavonoids, reducing sugars, terpinedes, and tannins. Different reactive oxidative species (ROS) including superoxide radicals and hydroxyl (OH) radicals are natural products produced in living organisms [ 7 ]. Reactive oxidative species produced as by product play a key role in cell signaling.…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical analysis and radical scavenging profile of juices of Citrus sinensis, Citrus anrantifolia, and Citrus limonum

2014

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BackgroundThe aim of the current investigation was to identify bioactive secondary metabolites including phenols, tannins, flavonoids, terpinedes, and steroids and compare the phytochemical analysis and antioxidant profile of the juice extracted from the fruits of Citrus sinensis, Citrus anrantifolia, and Citrus limonum.ResultsPhytochemical screening is important for the isolation of new, novel, and rare secondary metabolites before bulk extraction. Phytochemical analysis of the desired plant fruits of family Rutaceae revealed the presence of phenols, flavonoids, reducing sugars, steroids, terpinedes and tannins. The fruits of C. sinensis and C. anrantifolia exhibited the presence of phenols, flavonoids, reducing sugars, steroids, terpinedes and tannins, while the fruits of C. limonum indicated the presence of phenols, flavonoids, reducing sugars, terpinedes, and tannins. The fruits of selected plants were also subjected to antioxidant potential by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay against ascorbic acid at various concentrations. Among the tested plants, C. sinensis showed promising antiradical effect (84.81%) which was followed by C. Anrantifolia (80.05%) at 100 μg/ml against ascorbic acid (96.36%). The C. limonum showed low antioxidant activity among the three selected plants of family Rutaceae.ConclusionsThe current finding is baseline information in the use of the fruits of selected plants as food supplement which may be due to the presence of antioxidant molecules in the family Rutaceae. Further research is needed in this area to isolate the phenolic constituents which possess ideal antiradical potential.

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Section: Resultsmentioning

confidence: 99%

“…The antioxidant activity of the juices of C. sinensis , C. anrantifolia , and C. limonum was performed by DPPH radical scavenging assay according to standard assay protocol [ 7 ]. The positive control used in the current finding was ascorbic acid.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical analysis and radical scavenging profile of juices of Citrus sinensis, Citrus anrantifolia, and Citrus limonum

2014

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“…Different reactive oxidative species are natural products and are mostly produced by living organisms. [16] These free radicals can damage essential proteins, DNA, lipids, and shows harmful effects in cell structure and cause different kind of diseases/ disorders, i.e., cancer, stroke, and diabetes. Antioxidants play an important role against free radicals and provide a defense mechanism of living cells against oxidative damage.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

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Semwal¹

2018

IJGP

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Aim: The current study was designed to investigate total phenolic content (TPC), total flavonoid content (TFC), radical scavenging activity and antimicrobial profile of the aqueous, methanolic, ethanolic, and acetone leaf extract of Pittosporum eriocarpum. Materials and Methods: TPC was performed by the Folin-Ciocalteu method and TFC was measured by aluminum chloride assay. The free radical scavenging activity of leaf extracts of P. eriocarpum was ascertained by 1, 1-diphenyl-2-picrylhydrazyl assay and antimicrobial activity were studied by agar well diffusion method and were measured on the basis of the zone of inhibition (ZI) in millimeters. Results and Discussion: Screening of phytochemicals is a basic step in the investigation of the new potent bioactive component before huge extraction. P. eriocarpum leaf extracts possess significant results in terms of TPC and TFC, maximum value of TPC (544.60 ± 28.70 gallic acid equivalent [GAE] mg/g) was recorded in acetone extract while the minimum value of TPC (352.01 ± 32.4 GAE mg/g) was recorded in aqueous extract. The maximum (786.00 ± 13.75 QE mg/g) and minimum (493.30 ± 33.02 QE mg/g) values for TFC were recorded in methanolic and aqueous extracts, respectively. Radical scavenging activity was recorded to be maximum in the methanolic extract (66.91 ± 0.60%), whereas, lowest scavenging activity was recorded for aqueous extract (45.99 ± 0.7%), respectively. P. eriocarpum leaves extracts showed significant antimicrobial potential against three yeast strains (Candida albicans, Candida glabrata, and Candida tropicalis) and three bacterial strains (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). The maximum ZI was recorded in the methanolic extract (19.03 ± 0.47 mm) against Candida glabrata while minimum ZI 11.53 ± 0.41 mm for C. tropicalis was recorded. Methanolic extract showed maximum ZI of 14.67 ± 00.30 mm against P. aeruginosa while aqueous extract showed minimum ZI of 11.60 ± 00.65 mm for P. aeruginosa, respectively. Fluconazole (for fungal strains), ampicillin (for bacterial strains) and DMSO were used as a positive and negative control. Conclusion: These results may serve as the basic footsteps in the field of biomedical research, and further studies can be directed toward the investigation of novel molecules associated with its antioxidants, phytochemical properties, and antimicrobial properties.

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“…The plant is considered as rasayana and a vital ingredient of ayurvedic products because of its health promoting potentials via cell regeneration properties . Earlier reports described only a few molecules including β ‐sitosterol, lectins, diosgenin, 5‐hydroxymethyl‐2‐furaldehyde, santonin, 2‐hydroxybenzoic acid, homoisoflavonoids and polysaccharides from P. verticillatum . However, P. verticillatum has abundant demand in the global market but knowledge of the chemistry and a lack of reliable analytical methods can lead to the use of substitution and adulteration .…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

Sharma

Joshi

Kumar

2020

Phytochemical Analysis

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Introduction Polygonatum is widely used as a part of food in different regions of the world which covers five main categories such as drinks, vegetables, snacks, staple and seasoning foods. Presently, no analytical method is available for the quality control of Polygonatum. Objective Development and validation of a method using ultrahigh‐performance liquid chromatography diode array detector quadrupole time‐of‐flight (UHPLC‐DAD/QTOF) technique for the estimation of six compounds including a flavonol glycoside [rutin (1)], two flavonols [quercetin (2) and kaempherol (3)] and three homoisoflavonoids [5,7‐dihydroxy‐3‐(2‐hydroxy‐4‐methoxybenzyl)‐chroman‐4‐one (4), 5,7‐dihydroxy‐3‐(2‐hydroxy‐4‐methoxybenzyl)‐8‐methylchroman‐4‐one (5) and 5,7‐dihydroxy‐3‐(4‐methoxybenzyl)‐8‐methylchroman‐4‐one (6)]. In addition, screening of extract, fractions and compounds of P. verticillatum for antioxidant activity was also determined. Methodology The separation was achieved on C‐18 column using acetonitrile and water containing 0.1% formic acid. The method was validated as per ICH (International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use) guidelines. The validated method was applied for the simultaneous identification and quantification of compounds 1–6 in extract (E) and fractions (F1–F4) of P. verticillatum. Furthermore, antioxidant potential of E, F1 and F2 and compounds was evaluated using DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) assay. Results The method was within the linear range (r2) of 0.982 to 0.999, precise (intra‐ and inter‐day percentage relative standard deviations < 2.72 and 2.26) and accurate with recoveries (89.1–98.3%). The limit of detection and limit of quantification were in the ranges 0.02–0.16 and 0.06–0.48 ng/mL, respectively. Compounds 1–6 were quantified in all the samples. Compounds 1, 2 and 5 showed higher activity with half maximal inhibitory concentration (IC50) values 0.41, 0.39, 0.72 at 10, 20 and 30 μg/mL, respectively. Conclusion Developed method will be helpful to assess the quality of P. verticillatum raw material and their derived products.

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Antioxidant profile of constituents isolated from Polygonatumverticillatum rhizomes

Cited by 20 publications

References 16 publications

Phytochemical analysis and radical scavenging profile of juices of Citrus sinensis, Citrus anrantifolia, and Citrus limonum

Phytochemical analysis and radical scavenging profile of juices of Citrus sinensis, Citrus anrantifolia, and Citrus limonum

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Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

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