Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

Abstract

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

Analysis of toxic, antidiabetic and antioxidant potential of Bambusa balcooa Roxb. leaf extracts in alloxan-induced diabetic rats

“…Similar trend shown by the BAE and BME was very similar at 20, 80 and 120 lg/ml. Results were in accordance to previous works on other bamboo species (Goyal et al 2011a;Nagavani and Rao 2010). Mostly, the antioxidant activity in plants is due to the presence of polyphenols (Nakbi et al 2010;Middha et al 2013;Usha et al 2014).…”

“…Solvent selection was done based on the ethnopharmacological uses of the plants. Previous studies indicate methanol, water and acetone extraction as the most popular solvents used for the plant extraction research (Middha et al 2013(Middha et al , 2016aGoyal et al 2011aGoyal et al , b, 2013.…”

Antioxidant potential and total phenolic contents of seabuckthorn (Hippophae rhamnoides) pomace

“…A recent study by Kruczek et al [36] has showed the antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Seabuckthorn (Hippophae rhamnoides L.). Strong correlation was also observed between DPPH scavenging activity and total phenolic content from all Vinay Kant, et al: Antioxidant potential of seabuckthorn pomace the extracts of two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L. [37] In review of Patel et al, [38] it has been described that seabuckthorn reduced recurrence of angina, ischemic electrocardiogram which might be due to decreased myocardial oxygen consumption and inhibition of platelet aggregation induced by collagen. So, in addition of the antioxidant properties of these extract, they can be validated for the prevention of these complications.…”

Actinorhizal plants

“…Auguy et al (2011) provide the first (though preliminary) insights on the involvement of isoflavonoids as signal molecules during C. glauca nodulation. Regarding nodule functioning, Goyal et al (2011) discusses the antioxidant profiling of Hippophae salicifolia from the sacred forests of Sikkim, India. A detailed evaluation of the effects of elevated atmospheric CO 2 concentration, soil nutrients and water conditions on photosynthetic and growth responses of Alnus hirsuta is provided by Tobita et al (2011).…”