Antioxidant properties, antimicrobial and anti-adhesive activities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1

Abstract

BackgroundThe present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation.ResultsThe produced biosurfactants were characterized as lipopeptides molecules by using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). The DCS1 lipopeptides were assayed for their antioxidant activity through five different tests. The scavenging effect on DPPH radicals at a concentration of 1 mg mL−1 was 80.6%. The reducing power reached a maximum value of 3.0 (OD700 nm) at 2 mg mL−1. Moreover, the DCS1 lipopeptides exhibited a strong inhibition of β-carotene bleaching by linoleic acid assay with 80.8% at 1 mg mL−1 and showed good chelating ability and lipid peroxidation inhibition. The in vitro antimicrobial activity of DCS1 lipopeptides showed that they display significant antibacterial and antifungal activities. The anti-adhesive activity of DCS1 lipopeptides was evaluated against several pathogenic microorganisms. The lipopeptides showed excellent anti-adhesive activity, even at low concentrations, in a polystyrene surface pre-treatment against all the microorganisms tested. Further, they can disrupt performed biofilms.ConclusionThis study shows the potentiality of DCS1 lipopeptides as natural antioxidants, antimicrobial and/or anti-adhesive agent for several biomedical and industrial applications.

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

“…It has been, suggested that the free‐radical scavenging properties of the LPs help to prevent inflammation and improve tissue formation, re‐epithelization and differentiation of epidermis (Jemil et al . ). In addition, SPB1 has been shown previously to inhibit MDR bacteria (Ghribi et al .…”

Antioxidant properties of Enterobacter cloacae C3 lipopeptides in vitro and in model food emulsion

“…The inhibitor concentration IC 50 of C3 lipopeptides was estimated to be 37.5 μg/ml, which are more effective than lipopeptides produced by B. mojavensis A21 (Ben Ayed et al, ) and B. methylotrophicus DCS1 (Jemil et al, ), showing a IC 50 value of 3.7 mg/ml and 42 μg/ml, respectively.…”

“…In summary, the in vitro antioxidant capacities of C3 lipopeptides evaluated using different biochemical methods were found to be interesting. Many Gram‐positive bacteria including different species of the genus Bacillus are reported to produce antioxidant lipopeptides like B. methylotrophicus DCS1 (Jemil et al, ), B. mojavenis A21 (Ben Ayed et al, ), B. amyloliquefaciens An6 (Ben Ayed et al, ), B. subtilis (Tabbene et al, ; Zouari et al, ). This is the first report on antioxidant lipopeptides produced by Enterobacter strain.…”

“…To overcome these drawbacks, many natural molecules were studied for their antioxidant activities like protein hydrolysates from animal, plant, and aquatic products (Hmidet et al, ; Nasri et al, ). Among these biomolecules, biosurfactants were described for their antioxidant properties (Ben Ayed et al, ; Ben Ayed, Hmidet, Béchet, Jacques, & Nasri, ; Jemil, Ben Ayed, Manresa, Nasri, & Hmidet, ) and emulsions stabilization (Bai & McClements, ; Pekdemir, Çopur, & Urum, ).…”

A novel antioxidant and antimicrobial compound produced by Bacillus firmicutes

“…On the other hand, the methanol extract did not exhibit antimicrobial activity against E. coli. Several lipopeptide biosurfactants obtained from Bacillus amyloliquefaciens M1 [41], Bacillus mojavensis A21 [42] and Bacillus methylotrophicus [43] showed strong antibacterial activity. …”