Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan

Antioxidant, antihemolytic, and inhibitory activities of endemicPrimula heterochromaagainst Fe2+-induced lipid peroxidation and oxidative stress in rat brainin vitro

“…Determination of total phenolic content Total phenolic content of flavonoid-rich fractions of Primula heterochroma was determined using the FolinCiocalteau method (Kuda et al, 2005). Briefly, samples (0.5 mL, 1.6 mg/mL) were mixed with 2.5 mL of 0.2 N of FolinCiocalteau reagent for 5 min and 2.0 mL sodium carbonate (75 mg/mL) was then added.…”

“…Total flavonoid content was determined according to the method of Kuda et al (2005). Briefly, 0.5 mL of samples were mixed with 1.5 mL of methanol, 0.1 mL of aluminum chloride (10%), 0.1 mL of potassium acetate (1 M), and 2.8 mL of distilled water and incubated at 25°C (30 min).…”

“…Erythrocytes were isolated and stored by the method described by Kuda et al (2005). Separated erythrocytes were stored at 4°C and used within 6 h for further studies.…”

“…The amount of peroxide value was measured by thiocyanate method through recording absorbance at 480 nm after coloring with of FeCl 2 (0.02 M, 100 µL) and of ammonium thiocyanate (0.3 g/mL, 50 µL). Ascorbic acid was used as positive control (Kuda et al, 2005).…”

Enhancement of in Vitro Antioxidant Potential of Terminalia Chebula by Various Fruit Extracts and Optimization of Concentration by Response Surface Methodology

“…The screened combinations were subjected to DPPH free radical scavenging activity according to slight modifications in the protocol [17]. Briefly, 3.5 ml methanolic solution of 0.5 mM DPPH was added to 80 µl of various combinatorial concentrations of plant extract followed by incubation at 37°C for 20 min in the dark.…”