Antioxidant Properties of Stingless Bee Honey and Its Effect on the Viability of Lymphoblastoid Cell Line

Hazirah, H; My, Yasmin Anum; Karim, Norwahidah Abdul

doi:10.17576/mh.2019.1401.08

Cited by 15 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the disruptive effects of PS-MPs on uterine morphology were restored by concurrent treatment with Kelulut honey, which approximated normal histology. In addition to the carbohydrates, amino acids, proteins, organic acids, vitamins, and various phytochemicals contained in Kelulut honey, the improvement can be explained by the high content of natural antioxidants in honey [48].…”

Section: Discussionmentioning

confidence: 99%

“…increased antioxidant defenses, elevated Nrf2 expression, and decreased lipid peroxidation and oxidative DNA damage [50]. In addition, Kelulut honey has been shown to reduce oxidative stress (a precursor of DNA damage) in lymphoblastoid cell lines (LCLs) through its free radical scavenging activities (phenolic and flavonoid chemicals) [48]. Furthermore, Kek et al reported that Kelulut honey possesses higher phenolic content and has the highest antioxidant levels compared with Tualang and Gelam honey [17].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats

Amran

Zaid

Goh

et al. 2023

Toxics

View full text Add to dashboard Cite

Background: Microplastics (MPs) are an emerging global pollutant. Previous studies have revealed that chronic exposure to MPs can affect animal and human reproductive health, particularly by impairing the reproductive system’s normal functions, which may increase the risk of infertility in both males and females. Kelulut honey (KH), an excellent source of antioxidants, has been used to counteract the disruptive effects of Polystyrene microplastics (PS-MPs) in the rat uterus. Thus, this study aimed to investigate the potential protective effects of Kelulut honey against PS-MPs-induced uterine toxicity in pubertal rats. Methods: Prepubertal female Sprague Dawley rats were divided into four groups (n = 8): (i) normal control group (NC: treated with deionized water), MPs-exposed group (M: exposed to PS-MPs at 2.5 mg/kg), (iii) Kelulut honey group (DM: pretreated with 1200 mg/kg of KH 30 minutes before they were administered with PS-MPs at 2.5 mg/kg), and (iv) Kelulut honey control group (DC: only treated with KH at 2.5 mg/kg). The rats were treated orally once daily for six consecutive weeks. Results: Uterine abnormalities in PS-MPs-exposed rats were significantly improved after concurrent treatment with Kelulut honey. Morphology improvement was observed and luminal epithelial cells seemed thicker with more goblet cells, glandular cells had a more regular and circular shape, stromal cell increased in size, interstitial gaps between stromal cells expanded, and the myometrium layer was thicker. Kelulut honey treatment also effectively normalized the suppressive effect of PS-MPs on the expression and distribution of sex steroid receptors (ERα and ERβ), as well as the level of serum gonadotropin (LH and FSH) and sex steroid (estradiol and progesterone) hormones. Conclusion: Kelulut honey can protect the female reproductive system against the disruptive effects of PS-MPs. The phytochemical properties of Kelulut honey might be responsible for these beneficial benefits. However, future studies are warranted to identify the mechanisms involved.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats

Amran

Zaid

Goh

et al. 2023

Toxics

View full text Add to dashboard Cite

show abstract

“…enhanced anti-oxidant defenses, upregulated Nrf2 (protein that regulates the expression of antioxidant proteins), and reduced lipid peroxidation and oxidative DNA damage in in vivo study using Sprague Dawley rats. In addition, an in vitro study conducted by Abdul Karim [ 42 ] found that KH reduced the oxidative stress (precursor to DNA damage) in lymphoblastoid cell line (LCL) through its free radical scavenging activity (phenolic and flavonoid compounds). However, the protection to DNA damages by KH was only limited to irradiation doses below 20 Gy (11 Gy–19 Gy).…”

Section: Discussionmentioning

confidence: 99%

Radioprotective Effects of Kelulut Honey in Zebrafish Model

et al. 2021

View full text Add to dashboard Cite

Large doses of ionizing radiation can damage human tissues. Therefore, there is a need to investigate the radiation effects as well as identify effective and non-toxic radioprotectors. This study evaluated the radioprotective effects of Kelulut honey (KH) from stingless bee (Trigona sp.) on zebrafish (Danio rerio) embryos. Viable zebrafish embryos at 24 hpf were dechorionated and divided into four groups, namely untreated and non-irradiated, untreated and irradiated, KH pre-treatment and amifostine pre-treatment. The embryos were first treated with KH (8 mg/mL) or amifostine (4 mM) before irradiation at doses of 11 Gy to 20 Gy using gamma ray source, caesium-137 (137Cs). Lethality and abnormality analysis were performed on all of the embryos in the study. Immunohistochemistry assay was also performed using selected proteins, namely γ-H2AX and caspase-3, to investigate DNA damages and incidences of apoptosis. KH was found to reduce coagulation effects at up to 20 Gy in the lethality analysis. The embryos developed combinations of abnormality, namely microphthalmia (M), body curvature and microphthalmia (BM), body curvature with microphthalmia and microcephaly (BMC), microphthalmia and pericardial oedema (MO), pericardial oedema (O), microphthalmia with microcephaly and pericardial oedema (MCO) and all of the abnormalities (AA). There were more abnormalities developed from 24 to 72 h (h) post-irradiation in all groups. At 96 h post-irradiation, KH was identified to reduce body curvature effect in the irradiated embryos (up to 16 Gy). γ-H2AX and caspase-3 intensities in the embryos pre-treated with KH were also found to be lower than the untreated group at gamma irradiation doses of 11 Gy to 20 Gy and 11 Gy to 19 Gy, respectively. KH was proven to increase the survival rate of zebrafish embryos and exhibited protection against organ-specific abnormality. KH was also found to possess cellular protective mechanism by reducing DNA damage and apoptosis proteins expression.

show abstract

“…Antioxidant activity was measured using DPPH assay. 9 About 100 µL of the samples at different concentrations were mixed with the DPPH solution (Sigma-Aldrich, USA). The sample solutions were kept at room temperature for 30 mins.…”

Section: Dpph Scavenging Activitymentioning

confidence: 99%

“…About 7 mM of ABTS stock solution (Sigma-Aldrich, USA) was mixed with 2.45 mM ammonium persulphate (Sigma-Aldrich, USA) and incubated in the dark for 16 hours at room temperature. 9 Prior to assay, the mixture absorbance was adjusted to give 0.70±0.05 at 750 nm. ALLURA™ extract (30 µL) was added to 300 µL of ABTS working solution and let to stand in the dark for 6 min.…”

Section: Abts Radicals Assaymentioning

confidence: 99%

In vitro and In vivo Assessments on Nutraceutical and Safety of Herb-Fruit Based Drink on Female Wistar Rats

Hamid

Zakaria

Majid

et al. 2023

imjm

View full text Add to dashboard Cite

INTRODUCTION: The study aimed to assess the safety and nutraceutical properties of ALLURATM related to women’s health and skin beautification. MATERIALS AND METHODS: Determinations of total phenolic (TPC) and flavonoid contents (TFC) were done using the colorimetric method, followed by the identification of gallic acid via highperformance liquid chromatography (HPLC). Antioxidant activity was analyzed using 2,2 -diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radicals while its anti-inflammatory activity was measured using enzymelinked immunosorbent assay (ELISA). Anti-aging and whitening effects were determined by porcine elastase and mushroom tyrosinase activities, respectively. Skin cell growth promotion and rejuvenation were evaluated using in vitro scratch assay. Cytotoxicity assay was done using HSF1184 and 3T3 BALB/c cell lines. While, acute toxicity test was done on two groups (control and treatment) of six Wistar rats each. The nutraceutical properties were evaluated based on proximate analysis. RESULTS: ALLURATM exhibited DPPH-IC50 values of 180.40 µg/mL and ABTS-IC50 value of 174.40 µg/mL. TPC and TFC were 67.31 mg GAE/g extract and 43.21 mg CE/g extract, respectively while 10.98 mg/g of gallic acid were quantified. ALLURATM reduced pro-inflammatory cytokines of TNF-α and IL-6 and showed anti-aging (IC50-162.40 µg/mL) and whitening effects (IC50- 167.70 µg/mL). ALLURA™ also increased the proliferation of HSF1184 (≤ 1000 µg/ mL), producing significant secretion of epidermal growth factor (EGF) and shown to be non-cytotoxic. No mortality was observed at the highest dose of 2000 mg/kg b.w.t. nor the behavioral and morphological changes in rats. The proximate analysis resulted in high content of moisture and low calories. CONCLUSION: These findings provided preliminary reports for the first time on the functionality of ALLURA™ for its anti-inflammatory, antioxidant, and nutraceutical properties.

show abstract

Antioxidant Properties of Stingless Bee Honey and Its Effect on the Viability of Lymphoblastoid Cell Line

Cited by 15 publications

References 52 publications

Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats

Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats

Radioprotective Effects of Kelulut Honey in Zebrafish Model

In vitro and In vivo Assessments on Nutraceutical and Safety of Herb-Fruit Based Drink on Female Wistar Rats

Contact Info

Product

Resources

About