Antioxidant Properties of Volatile Oil of Indian Propolis

Abstract

Antioxidant properties shown by volatile oil of propolis (VOP) from India were investigated by spectrophotometric methods (ABTS, DPPH and NO radical scavenging activity) and a photochemiluminescence (PCL) method (superoxide scavenging activity). It was seen that VOP exhibited superior TEAC activity to that of trolox at the concentration 0.2 mg/mL. It showed dose dependent DPPH radical scavenging activity and NO scavenging activity. It was observed that the overall NO scavenging activity shown by VOP was low which suggests that some of the compounds responsible for NO radical scavenging might be nonvolatile and might not be present in VOP. The superoxide scavenging activity of VOP was determined on nano scale by PCL method. These results indicate that superoxide scavenging activity was more efficient in dilute solutions of VOP. The polyphenol content in the VOP samples was also determined. It was seen that polyphenol content may not be the exclusive cause for the antioxidant activity. From IC 50 values it could be concluded that the efficiency of scavenging ABTS radicals by the VOP was more pronounced as compared to scavenging other radicals. These findings indicate that VOP could be an easily accessible source of natural antioxidants.

Standardization, anti-carcinogenic potential and biosafety of Indian propolis

“…Further, mark was extracted with water by Soxhlet extraction at 100 °C to obtain water extract of Indian propolis (WEIP). All extracts were stored in 2–8 °C and used for further evaluation [24].…”

“…Although propolis is extensively studied globally, the research on Indian propolis is at infancy. There is lack of studies on extraction optimization, analytical method and biosafety study on Indian propolis except few [7], [18], [24], [25], [26], [27]. Hence, in the present study, an attempt has been made for extraction of Indian propolis by suitable method, standardization with respect to selected markers by newly developed reversed phase high performance liquid chromatography (RP-HPLC) method, evaluation of in vitro and in vivo anti-cancer activity to study synergistic effects of CAPE with other polyphenols and flavonoids and biosafety to explore Indian propolis as a biomedicine.…”

Antioxidant Activity of Indian Propolis - An<i> In Vitro </i>Evaluation

“…Worldwide propolis has a tremendous popularity and has become a subject of interest concerning the chemistry and biological activities. On the other hand, the variation in the chemistry of propolis from different ecosystems has made it a source of active molecules mainly as antimicrobial [3], anti-inflammatory [4], antioxidant [5], hepatoprotective [6], immunostimulating [7] and cytotoxic [8,9]. Propolis contains more than 300 chemical constituents in which polyphenol and flavonoids play a role in the pharmacological activities.…”