Antioxidant Properties of Water-Soluble Gum from Flaxseed Hulls

Abstract

Soluble flaxseed gum (SFG) was extracted from flax (Linum usitatissimum) hulls using hot water, and its functional groups and antioxidant properties were investigated using infrared spectroscopy and different antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), reducing power capacity, and β-carotene bleaching inhibition assay), respectively. The antioxidant capacity of SFG showed interesting DPPH radical-scavenging capacity (IC50 SFG = 2.5 mg·mL−1), strong ABTS radical scavenging activity (% inhibition ABTS = 75.6% ± 2.6% at 40 mg·mL−1), high reducing power capacity (RPSFG = 5 mg·mL−1), and potent β-carotene bleaching inhibition activity (IC50 SFG = 10 mg·mL−1). All of the obtained results demonstrate the promising potential use of SFG in numerous industrial applications, and a way to valorize flaxseed hulls.

Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity

“…The significantly higher reducing power was noticed at a concentration of 30 mg/ml ( A 700nm = 0.83) and lower at control or 0 concentration ( A 700nm = 0.18) and at a concentration of 5.0 mg/ml ( A 700nm = 0.27). Results are in agreement (but higher in reductive capability rate) with Bouaziz et al () who noticed the same increasing trend of reducing power values with increasing concentration in soluble FSG. Furthermore, Zhang et al () observed the correlation of reducing power with increasing concentrations for five algae polysaccharide.…”

“…Comparatively, Liang et al (2017) extracted FSG oligosaccharide by the oxidative degradation of H 2 O 2 and found ABTS radical scavenging rate of 91.2% at 25 mg/ml that is higher than the values obtained in the current study. Moreover, Bouaziz et al (2016) isolated soluble FSG from the flaxseed hull having the scavenging ABTS radicals of 75.6% similar in our case but at a higher FSG concentration of 40 mg/ml.…”

“…The significantly higher TAC was measured at 30 mg/ml (588 µg·TE·ml −1 ) and significantly lower at control or 0 concentration (54 µg·TE·ml −1 ) and at 5 mg/ml (201 µg·TE·ml −1 ). Bouaziz et al (2016) observed the same increasing trend of total antioxidant values with a rising concentration of FSG up to 10 mg/ml.…”

“…Nevertheless, the existence of side effects is almost inevitable. As a result, there is a growing interest in finding new antioxidant supplements, specially natural or antioxidant compound from natural resources like FSG to limit the detrimental effects of reactive oxygen species (ROS) to human body (Li et al, ) but less concern has been paid to antioxidant activities of FSG (Bouaziz et al, ).…”

RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

“…An antioxidant molecule is able to reduce the stable radical DPPH, having a deep violet color in solution, to the yellow-colored 1,1-diphenyl-2-picrylhydrazyl (DPPH 2 ) [56]. The results showed that ethanol and water extracts of all samples showed moderate radical scavenging activity when compared to standard antioxidants (Figure 2C,F).…”