Flaxseed gum: Extraction, bioactive composition, structural characterization, and its potential antioxidant activity

Abstract

Flaxseed gum (FSG) is a heteropolysaccharide consist of neutral and acidic components that makes up approximately 8% of seed mass. FSG was extracted from mixture of brown and golden varieties of flaxseeds by hot water extraction method. The molecular weight distribution, monosaccharide analysis, chemical composition, and surface morphology of FSG were scrutinized in the current study to get a better insight regarding this important polysaccharide. The average molecular weight was recorded as 1,322 kDa with a polydispersity ratio of 1.6 for Mw/Mn and 2.4 for Mn/Mz. High‐performance liquid chromatography (HPLC) screening revealed that extracted FSG was comprised of rhamnose, arabinose, mannose, glucose, fucose, xylose, galactose, glucosamine, glucuronic acid, and galacturonic acid, of which mannose and glucosamine have not been reported previously. The antioxidant activities of FSG measured as DPPH, ABTS, reducing power, and total antioxidant activity clearly demonstrated the antioxidant potency of FSG. Fourier‐transform infrared spectroscopy (FTIR) and the Nuclear magnetic resonance (NMR) (1H, 13C) s pectra ratified the presence of functional groups typical for polysaccharide. Practical applications Flaxseed gum is a natural carbohydrate polymar. This study provides useful information regarding antioxidant potential, chemical, and structural characterization of FSG. The availability of a gum is fully characterized with bioactive composition, structural features, and antioxidant potential provides a toolset for the practical application in the food or drug industry.

In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed

“…Flaxseed ( Linum usitatissimum L.) contains various of biological compounds such as n-3 fatty acids, good quality dietary fiber and bioactive lignans, which was considered as a good functional food ingredients [ 15 , 16 ]. The main soluble dietary fiber extracted from flaxseed is a heterogeneous polysaccharide, which consist of neutral arabinoxylan and acidic rhamnose [ 17 ]. Flaxseed polysaccharide (FSP) exhibited good antioxidant activities when scavenging radicals in vitro [ 18 ].…”

The Application of Spray-Dried and Reconstituted Flaxseed Oil Cake Extract as Encapsulating Material and Carrier for Probiotic Lacticaseibacillus rhamnosus GG

“…Therefore, the complete maintenance of the structural and functional integrity of the bacterial cell membrane during the spray drying process may be disrupted [ 43 ]. FP and FG are high molecular weight polymers [ 17 , 70 , 71 ] and therefore most likely interact directly with polar groups of cell wall peptidoglycan, lipoteichoic acids and teichoic acids, thus providing stability to bacterial cells [ 1 , 71 ]. Presumably, high water holding activity of FOCE (due to FG content) was also an important factor for LGG survivability [ 35 ].…”

Dietary Fiber: Fractionation, Characterization and Potential Sources from Defatted Oilseeds

“…Alginates from brown seaweeds and carrageenans from red seaweeds NMR, FTIR and SEC [40] Soluble DF from black soybean hulls Monosaccharides composition, molecular weight, FTIR, SEM [45] Nettle seed gum FTIR analysis and monosaccharide composition [46] Soluble DF from wheat bran Molecular weight (SEC-MALLS), monosaccharide composition, FTIR, SEM [47] Galactomannan from Prosopis ruscifolia seeds Monosaccharide composition (GC-MS), structure (NMR) and viscosity molecular weight (Huggins plot) [48] Indigestible carbohydrates from wheat processing AEC, SEC, NMR [49] Flaxseed gum Molecular weight distribution, monosaccharide composition (HPLC), FTIR, NMR [50] Table 1. Cont.…”