Antioxidant protection during ageing and senescence in chloroplasts of tobacco with modulated life span

Procházková, Dagmar; Haisel, D.; Wilhelmová, N.

doi:10.1002/cbf.1481

Cited by 27 publications

(24 citation statements)

References 55 publications

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“…and Amasino, 1995;Procházková et al, 2008). Concomitantly, 35S:GRF5 plants accumulate high levels of ascorbate (Gonzalez et al, 2010).…”

Section: Effects Of Grf5 and Cytokinins On Senescence And Nitrogen Mementioning

confidence: 94%

“…Chl levels were also decreased in the shoot of the B-type arr1 arr10 arr12 triple mutant, affected in the majority of cytokininactivated responses during vegetative plant development (Argyros et al, 2008;Ishida et al, 2008). In addition, ectopic expression of the bacterial cytokinin biosynthetic ISOPENTENYLTRANSFERASE (ipt) gene increased Chl levels, enhanced photosynthetic activity, affected the ultrastructure of chloroplasts, and delayed leaf senescence (Synková et al, 2006;Procházková et al, 2008;Cortleven and Valcke, 2012).…”

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confidence: 99%

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GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity

et al. 2015

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Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplasts, providing the plant with energy. The transcription factor GROWTH REGULATING FACTOR5 (GRF5) promotes the duration of the cell proliferation period during leaf development. Here, it is shown that GRF5 also stimulates chloroplast division, resulting in a higher chloroplast number per cell with a concomitant increase in chlorophyll levels in 35S:GRF5 leaves, which can sustain higher rates of photosynthesis. Moreover, 35S:GRF5 plants show delayed leaf senescence and are more tolerant for growth on nitrogen-depleted medium. Cytokinins also stimulate leaf growth in part by extending the cell proliferation phase, simultaneously delaying the onset of the cell expansion phase. In addition, cytokinins are known to be involved in chloroplast development, nitrogen signaling, and senescence. Evidence is provided that GRF5 and cytokinins synergistically enhance cell division and chlorophyll retention after darkinduced senescence, which suggests that they also cooperate to stimulate chloroplast division and nitrogen assimilation. Taken together with the increased leaf size, ectopic expression of GRF5 has great potential to improve plant productivity.

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“…and Amasino, 1995;Procházková et al, 2008). Concomitantly, 35S:GRF5 plants accumulate high levels of ascorbate (Gonzalez et al, 2010).…”

Section: Effects Of Grf5 and Cytokinins On Senescence And Nitrogen Mementioning

confidence: 94%

mentioning

confidence: 99%

GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity

et al. 2015

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“…Data were captured and calculated by PC-software Clarity (DataApex, Prague, Czech Republic). Chlorophyll fluorescence parameters from slow kinetics were measured after a 15 min dark period with the PAM Chl fluorometer (Walz, Effeltrich, Germany) on the adaxial side of fresh leaves as described by Procházková et al [30], using the DA 100 data acquisition system (Walz, Effeltrich, Germany) for sampling and calculation.…”

Section: Methodsmentioning

confidence: 99%

The impact of increased soil risk elements on carotenoid contents

et al. 2014

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A pot experiment was conducted to compare the responses of a non-transgenic tobacco plant (WT) and plants with genetically prolonged life-span (SAG) to risk elements of As, Cd and Zn. Plants were grown in control soil and in soil with higher levels of risk elements. The pigment contents were established by HPLC and chlorophyll fluorescence parameters were measured from slow kinetics after a 15 min dark period with the PAM fluorometer. Top (i.e. young) leaves of both WT and SAG plants were more sensitive to photoinhibition caused by these risk elements but plants showed acclimation to such elements in the bottom leaves. Plants differed in the participation of individual pigments of xanthophyll cycle: increased levels of risk elements seem to stimulate especially first (violaxanthin to antheraxanthin) and second (anhtheraxanthin to zeaxanthin) steps of the cycle in WT plants. In SAG plants, toxic elements caused an increase in the content, particularly of the initial compound of the cycle — violaxanthin.

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“…After in vitro precultivation, plants were cultivated for 60 days in the polluted soil (Gleyic Cambisol -pH = 6.1, CEC = 134 mmol (+) /kg, C org = 2.1%) from the site Příbram (highly polluted mainly by atmospheric emissions from the smelter), marked as PS and in nonpolluted soil (Chernozem -pH = 7.2, CEC = 258 mmol (+) /kg, C org = 1.8%) from the site PragueSuchdol, marked as CS, both in Central Bohemia, Czech Republic. Plants were grown in greenhouse as described in Procházková et al (2008). We employed two individual groups of samples; each was comprised of the mixture of six leaves per each group.…”

Section: Methodsmentioning

confidence: 99%

“…Pigment contents were established by HPLC (ECOM, Prague, Czech Republic) from acetone extracts using a reversed phase column Sepharon SGX C18 (Tessek, Praha, Czech Republic), data were captured and calculated by PC-software Clarity (DataApex, Prague, Czech Republic) (Procházková et al 2008). Chlorophyll fluorescence parameters from slow kinetics were measured after a 15 min dark period with the PAM Chl fluorometer (Walz, Effeltrich, Germany) on adaxial side of fresh leaves, using the DA 100 data acquisition system (Walz, Effeltrich, Germany) for sampling and calculation (Wilhelmová et al 2005).…”

Section: Methodsmentioning

confidence: 99%

The effect of risk elements in soil to nitric oxide metabolism in tobacco plants

Procházková¹,

Haisel²,

Pavlı́ková³

et al. 2012

PLANT SOIL ENVIRON

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We studied changes of endogenous nitric oxide content (NO) and of reactive nitrogen species metabolism in transgenic tobacco with prolonged life span (SAG) and in wild tobacco (WT) cultivated in the control and in the polluted soil. There was no difference in the metal accumulation between WT and SAG plants however SAG ones showed better ability to cope with risk elements, as they retained higher membrane stability index and chlorophyll content together with better photochemical efficiency and lower deepoxidation status. Risk elements induced higher NO production in the youngest leaves of both plant types. Low and middle leaves of both WT and SAG plants showed similar activities of nitrate reductase and nitrosoglutathione reductase. Increase of nitrotyrosine content in leaf soluble proteins suggests that risk elements induced nitrosative stress in both plant types.

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Antioxidant protection during ageing and senescence in chloroplasts of tobacco with modulated life span

Cited by 27 publications

References 55 publications

GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity

GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity

The impact of increased soil risk elements on carotenoid contents

The effect of risk elements in soil to nitric oxide metabolism in tobacco plants

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