Antioxidant response of Calendula officinalis L. assisted synthesized zinc oxide nanoparticles

Abstract

The over-production of free radicals in the body causes oxidant damage in the body. Currently, zinc oxide nanoparticles (ZnO NPs) are gaining the attention of most scientists because of their excellent physical, chemical, and biological properties. In this work, the ZnO NPs were synthesized using the petal extract of C. officinalis L. An absorbance spectrum of the synthesized ZnO NPs was recorded using an ultraviolet-visible (UV-Vis) spectrophotometer. The absorbance band around 368 nm confirms the formation of ZnO NPs. The transmission electron microscopy (TEM) analysis clearly shows that the most of the ZnO NPs is spherical in shape with average particle size ~ 85 nm. The field emission scanning electron microscope (FE-SEM) result demonstrates the spherical morphology with large agglomeration of the particles. The energy-dispersive X-ray spectroscopy (EDS) result confirms the presence of Zn in the synthesized nanomaterials. The X-ray diffraction (XRD) peaks represent the crystalline structure of the ZnO NPs with average particle size ~ 27.22 nm. Fourier transform infra-red (FT-IR) spectrum of synthesized ZnO NPs was recorded in the range of 4000-500 cm-1.The ester and carboxylic groups were found at various sharp bands at 1017 cm-1, 952 cm-1, 688 cm-1, 609 cm-1, and 514 cm-1 due to the presence of Zn-O band stretch in the FT-IR spectrum. Further synthesized material was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay to evaluate its antioxidant activity. It showed that ZnO NPs exhibited significant antioxidant activity through scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Thus, it could be seen that the synthesis of naturally occurring plant product ZnO NPs acts as an alternative chemical antioxidant. Hence, the herbal synthesized ZnO NPs are proven to be a potent antioxidant agent and can be used in several medicinal applications.