Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats

Attia, Azza A.; ElMazoudy, Reda H.; El-Shenawy, Nahla S.

doi:10.1016/j.pestbp.2012.04.002

Cited by 53 publications

(34 citation statements)

References 41 publications

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“…In the present finding, MD intoxication caused a significant increase in the status of the serum ALT, AST, ALP and LDH activities and that might be related to the release of these enzymes from the cytoplasm into the blood stream rapidly after the rupture of plasma membranes and cellular damage caused by the free radicals produced during the MD metabolism. These results are consistent with the previous findings realized by some researchers who had found an association between OPIs toxicity and the increased oxidative stress of rats (Sefi et al, 2011;Attia et al, 2012). Consequently, these biochemical changes could be correlated with histological alterations.…”

Section: Discussionsupporting

confidence: 93%

“…A significant decrease of GST activity in rats treated with MD was observed. This is in good accordance with the study of Attia et al (2012), which correlate the significant reduction of GST by the decrease in GSH and glutathione dependent enzyme system. The actual results revealed that MD caused a statistically significant decrease of liver SOD activity.…”

Section: Discussionsupporting

confidence: 92%

“…The latter represent the chemical mediator of nervous system, and it is responsible for the transmission of nervous function by binding to post synaptic acetylcholine receptors, resulting in muscle contraction or glands secretions (Lauwerys and Lisson, 2007;Abou-Donia, 2010). Chronic and subchronic exposure of OPIs may induce oxidative stress leading to the generation of free radicals and alteration in antioxidants or reactive oxygen species (ROS) scavenging enzymes (Attia et al, 2012). Methidathion (ultracide, GS 13005) S-[(2, 3-dihydro-5-methoxy-2-oxo-1, 3, 4-thiadiazol 3(2H)-yl) methyl] O,O dimethyl phosphorodithioate) (Figure 1) is one of the most widely used OPIs for agriculture and public health programmes (Yavuz et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

Barkat¹,

Boumendjel²,

Abdennour³

et al. 2015

Afr. J. Biotechnol.

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The aim of this study was to determine the effect of a sub-acute 4-week exposure to methidathion (MD) on the hematological system and hepatic integrity of rats. We also assessed whether co-administration of micronutrients such as selenium (Se) and zinc (Zn) prevented MD-induced hepatic damage. Rats were randomly divided into four groups of six each: the first group served as a control which received standard diet, the second group received both Se (0.5mg/kg of diet) and Zn (0.227 g/l drinking water), the third group received only MD (5 mg/kg b.w. by gavage using corn oil as a vehicle), and the fourth group received MD and combined selenium and zinc. After four weeks, exposure to MD induced a significant increase in plasmatic activities of AST, ALT, ALP, LDH, and liver malondialdehyde level. In contrast, reduced glutathione level (GSH), and the activities of catalase (CAT), superoxide dismutase (SOD), and the glutathione peroxidase (GPx) content of hepatic tissue decreased significantly. Moreover, treatment with Se and Zn in MD-treated rats maintained all the biochemical parameters cited above. In conclusion, the obtained results indicate the ability of Se and Zn to attenuate the MD-induced liver and erythrocytes oxidative damage.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

Barkat¹,

Boumendjel²,

Abdennour³

et al. 2015

Afr. J. Biotechnol.

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“…induces oxidative stress in the plasma of rats. The oxidative stress induced by rats' exposure to the CPF in this study has also been reported in other similar studies carried out for mice or rats [7,[22][23][24].…”

Section: Discussionsupporting

confidence: 88%

“…Organophosphates may induce injuries in various organs and cells [3,[7][8][9]24]. The exhibited histopathological changes due to the CPF induced liver toxicity were attenuated by the CAPE and CAPE+IL treatment, but not by exclusively the IL treatment.…”

Section: Histopathological Evaluationmentioning

confidence: 96%

Attenuating effects of caffeic acid phenethyl ester with intralipid on hepatotoxicity of chlorpyrifos in the case of rats

Dokuyucu¹,

Bilgili²,

Hanedan³

et al. 2016

Med Pr

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Background: Chlorpyrifos (CPF), insecticide widely used in agriculture, may cause poisonings in the case of humans. As a result, there is a large amount of treatment research underway to focus on the possibility of chlorpyrifos induced poisonings. The aim of this study has been to evaluate the effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity induced by chlorpyrifos in the case of rats. Material and Methods: The rats in this study were treated with CPF (10 mg/kg body weight (b.w.), orally), CAPE (10 µmol/kg b.w., intraperitoneally), IL (18.6 ml/kg b.w., orally), CPF+CAPE, CPF+IL, and CPF+CAPE+IL. The plasma total oxidant capacity (TOC), total antioxidant capacity (TAC) were measured and the oxidative stress index (OSI) was calculated. Liver histopathology and immunohistochemical staining were performed. Results: Chlorpyrifos statistically significantly decreased the TAC levels in the rats' plasma and increased the apoptosis and the TOC and OSI levels. In the chlorpyrifos induced liver injury, CAPE and CAPE+IL significantly decreased the plasma OSI levels and the apoptosis, and significantly increased the plasma TAC levels. Conclusions: This study revealed that CAPE and CAPE+IL attenuate chlorpyrifos induced liver injuries by decreasing oxidative stress and apoptosis. Med Pr 2016;67(6):743-749

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LC/MS/MS Phenolic Profile, Antioxidant Capacity, Angiotensin‐Converting Enzyme (ACE) and Glutathione S Transferase (GST) Inhibitory Properties of Ultrasound‐Assisted Propolis Extracts

Aras

Korkmaz

Bayrakdar

2023

Chemistry & Biodiversity

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Resinous beehive product propolis has many biological activities. It contains various aromatic substances that have great differences in their chemical composition depending on the natural flora. Thus, chemical characterization and biological properties of propolis samples is an important subject for the pharmaceutical industry. In this study, the propolis samples collected from three cities in Turkey were prepared as methanol (MEP), ethanol (EEP), chloroform (ChlEP), hexane (HxEP), and ethyl acetate (EAEP) extracts using an ultrasonic assisted technique. The antioxidant capacities of the samples were evaluated by free radical scavenging activity (DPPH), cation radical scavenging activity (ABTS), and reducing activity (CUPRAC) and (FRAP). The strongest biological activities were detected in ethanol and methanol extracts. Enzyme inhibition of the propolis samples were determined against the human glutathione S-transferase (GST) and angiotensin converting enzyme (ACE). IC 50 values of MEP1, MEP2, and MEP3 samples against the ACE were found as 13.9 μg/mL, 14.8 μg/mL, and 12.8 μg/mL, while against the GST IC 50 values of MEP1, MEP2, and MEP3 samples were as 5.92 μg/mL, 9.49 μg/ mL, and 5.72 μg/mL. To know the possible causes of the biological test results advanced LC/MS/MS method was applied. trans-ferulic acid, kaempferol, and chrysin were found as the most abundant phenolic compounds in each sample. The propolis extracts obtained using the proper solvent have a good potential to be used in pharmaceuticals to treat the diseases related to oxidative damage, hypertension, and inflammation. Finally, the interactions between chrysin, trans-ferulic acid and kaempferol molecules with ACE and GST receptors were analyzed using molecular docking study. Selected molecules interact with active residues by binding to the active site of the receptors.

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Antioxidant role of propolis extract against oxidative damage of testicular tissue induced by insecticide chlorpyrifos in rats

Cited by 53 publications

References 41 publications

Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

Attenuating effects of caffeic acid phenethyl ester with intralipid on hepatotoxicity of chlorpyrifos in the case of rats

LC/MS/MS Phenolic Profile, Antioxidant Capacity, Angiotensin‐Converting Enzyme (ACE) and Glutathione S Transferase (GST) Inhibitory Properties of Ultrasound‐Assisted Propolis Extracts

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