Antioxidant role of α-lipoic acid in lead toxicity

Gürer, Hande; Özgüneş, Hilâl; Öztezcan, Serdar; Erçal, Nuran

doi:10.1016/s0891-5849(99)00036-2

Cited by 104 publications

(59 citation statements)

References 26 publications

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“…Our previous studies (Ercal et al 1996;Gurer et al 1998Gurer et al , 1999a as well as the present one support the hypothesis indicated by several other groups (Ribarov and Bochev 1982;Ito et al 1985;Monterio et al 1985Monterio et al , 1991Lawton and Donaldson 1991;Sandhir et al 1994;Solliway et al 1996) that Fig. 2.…”

Section: Discussionsupporting

confidence: 92%

“…Decreased cell survival in leadexposed CHO cells was accompanied by increased lipid peroxidation and altered antioxidant defense systems, suggesting oxidative stress as a possible mechanism for lead-induced damage. Furthermore, in one of our earlier studies, treatment of lead-exposed CHO cells with superoxide dismutase ϩ CAT was found to reverse MDA and GSH levels back to control levels indicating that part of this injury may be mediated by ROS (Gurer et al 1999a). The possible curicial role of oxidative stress in the pathophysiology of lead toxicity brought up the idea that induced damage could be relieved in part by antioxidants.…”

Section: Discussionmentioning

confidence: 87%

“…The possible curicial role of oxidative stress in the pathophysiology of lead toxicity brought up the idea that induced damage could be relieved in part by antioxidants. We previously suggested some thiol antioxidants such as N-acetylcysteine (NAC), lipoic acid, and captopril to restore the impaired prooxidant/antioxidant balance in lead toxicity (Ercal et al 1996;Gurer et al 1998Gurer et al , 1999aGurer et al , 1999b. The antioxidant activity of succimer, a potent chelating agent with two free thiol groups, was also investigated in our lab (Ercal et al 1996;Gurer et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown that N-acetylcysteine and lipoic acid, two well-known antioxidants, are effective in rebalancing the oxidant/antioxidant ratio in lead-exposed Chinese hamster ovary (CHO) cells and Fisher 344 (F344) rats (Ercal et al 1996;Gurer et al 1998Gurer et al , 1999a. Taurine, a sulfur-containing ␤-amino acid (Figure 1) is found in millimolar concentrations, especially in tissues that are excitable, rich in membranes, and generate oxidants (Jacobsen and Smith 1968;Wright et al 1986).…”

mentioning

confidence: 99%

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Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Gürer

Özgüneş

Saygın

et al. 2001

Arch. Environ. Contam. Toxicol.

139

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Abstract. Oxidative stress is proposed as a molecular mechanism in lead toxicity, which suggests that antioxidants might play a role in the treatment of lead poisoning. The present study was designed to investigate whether taurine has a beneficial effect both on Chinese hamster ovary (CHO) cells and on Fisher 344 (F344) rats following lead exposure. Therefore, oxidative stress parameters (glutathione, malondialdehyde levels, catalase, and glucose-6-phosphate dehydrogenase [G6PD] activities) of lead-exposed CHO cells and F344 rats were determined following taurine treatment. Taurine was found to be effective in (1) increasing glutathione levels that had been diminished by lead; (2) reducing malondialdehyde levels, an end-product of lipid peroxidation; (3) decreasing catalase and erythrocyte G6PD activity, which had been increased by lead exposure; and (4) improving cell survival of CHO cells. However, taurine had no effect on blood and tissue lead levels when 1.1 g/kg/day taurine was administered to F344 rats for 7 days, following 5 weeks of lead exposure (2,000 ppm lead acetate). As a result, taurine seems to be capable of fortifying cells against lead-induced oxidative attack without decreasing lead levels. Therefore, administration of taurine, accompanied by a chelating agent, might increase its effectiveness in the treatment of lead poisoning.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Gürer

Özgüneş

Saygın

et al. 2001

Arch. Environ. Contam. Toxicol.

139

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“…We found very little role of melatonin in the mobilization of lead but it provided significant protection against lead induced oxidative stress in tissues of lead exposed rats. α α α α α-Lipoic acid (LA) α-Lipoic acid is a naturally occurring antioxidant and is able to abate some of the toxic effects of lead 202) . It functions as a cofactor in several multienzyme complexes 203) .…”

Section: Melatoninmentioning

confidence: 99%

Strategies for Safe and Effective Therapeutic Measures for Chronic Arsenic and Lead Poisoning

Kalia¹,

Flora²

2005

Journal of Occupational Health

269

161

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Strategies for Safe and Effective Therapeutic Measures for Arsenic and LeadPoisoning: Kiran Kalia, et al. Department of Biosciences, Sardar Patel University, IndiaExposure to toxic metals remains a widespread occupational and environmental problem in world. There have been a number of reports in the recent past suggesting an incidence of childhood lead poisoning and chronic arsenic poisoning due to contaminated drinking water in many areas of West Bengal in India and Bangladesh has become a national calamity. Low level metal exposure in humans is caused by air, food and water intake. Lead and arsenic generally interferes with a number of body functions such as the central nervous system (CNS), the haematopoietic system, liver and kidneys. Over the past few decades there has been growing awareness and concern that the toxic biochemical and functional effects are occurring at a lower level of metal exposure than those that produce overt clinical and pathological signs and symptoms. Despite many years of research, we are still far from an effective treatment of chronic plumbism and arsenicosis. Medical treatment of acute and chronic lead and arsenic toxicity is furnished by chelating agents. Chelating agents are organic compounds capable of linking together metal ions to form complex ring-like structures called chelates. They have been used clinically as antidotes for acute and chronic poisoning. 2, 3-dimercaprol (BAL) has long been the mainstay of chelation therapy for lead or arsenic poisoning. Meso 2, 3, -dimercaptosuccinic acid (DMSA) has been tried successfully in animals as well as in a few cases of human lead and arsenic poisoning. DMSA could be a safe and effective method for treating lead or arsenic poisoning, but one of the major disadvantages of chelation with DMSA has been its Review inability to remove lead from the intracellular sites because of its lipophobic nature. Further, it does not provide protection in terms of clinical/ biochemical recovery. A new trend in chelation therapy is to use combined treatment. This includes the use of structurally different chelators or a combination of an adjuvant and a chelator to provide better clinical/ biochemical recovery in addition to lead mobilization. The present review article attempts to provide update information about the current strategies being adopted for a safe, effective and specific treatment for two major toxic metals or metalloid.

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Oxidative stress‐alleviating strategies to improve recombinant protein production in CHO cells

Chevallier

Andersen

Malphettes

2019

Biotech & Bioengineering

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Large scale biopharmaceutical production of biologics relies on the overexpression of foreign proteins by cells cultivated in stirred tank bioreactors. It is well recognized and documented fact that protein overexpression may impact host cell metabolism and that factors associated with large scale culture, such as the hydrodynamic forces and inhomogeneities within the bioreactors, may promote cellular stress. The metabolic adaptations required to support the high‐level expression of recombinant proteins include increased energy production and improved secretory capacity, which, in turn, can lead to a rise of reactive oxygen species (ROS) generated through the respiration metabolism and the interaction with media components. Oxidative stress is defined as the imbalance between the production of free radicals and the antioxidant response within the cells. Accumulation of intracellular ROS can interfere with the cellular activities and exert cytotoxic effects via the alternation of cellular components. In this context, strategies aiming to alleviate oxidative stress generated during the culture have been developed to improve cell growth, productivity, and reduce product microheterogeneity. In this review, we present a summary of the different approaches used to decrease the oxidative stress in Chinese hamster ovary cells and highlight media development and cell engineering as the main pathways through which ROS levels may be kept under control.

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Antioxidant role of α-lipoic acid in lead toxicity

Cited by 104 publications

References 26 publications

Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Strategies for Safe and Effective Therapeutic Measures for Chronic Arsenic and Lead Poisoning

Oxidative stress‐alleviating strategies to improve recombinant protein production in CHO cells

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