Antioxidant Supplementation and Kidney Function Status of Wistar Rats Following High Fat Diet-Streptozotocin (HFT-STZ) Induced Type 2 Diabetes

Abstract

The kidney function status of high fat diet-streptozotocin (HFD-STZ) induced NIDDM in albino rats fed antioxidant supplementation was monitored in vitro. Appropriate and recommended dietary allowed proportions of some potent antioxidant substances including: minerals, vitamins, α-lipoic acid, phytochemicals and a D-ribose-Lcysteine conjugate were assembled together in corn oil and stored at 4°C for use. Kidney function indices were assayed using standard methods, kits and equipments. Data analysis was done with SPSS version 20.0 and significant level was set at p ≤ 0.05. There were a total of five study groups with 10 rats each. Immediately after the induction of diabetes with HFD-STZ combination, treatment commenced and lasted for a total of 12 weeks, and analysis using serum was carried out at the 4 th , 8 th and 12 th week of the study. Results obtained from the kidney function status investigation indicates that there was significant decrease (p ≤ 0.05) in serum urea levels of the treated groups when compared to the controls (normal and diabetic) and this decrease was consistent as the treatment progressed. Serum creatinine, biocarbonate and potassium levels of both the treated and normal control groups where not statistically different (p ≥ 0.05) when compared with the diabetic control group which increased steadily for creatinine and biocarbonate, but inconsistent for potassium level within the treatment duration. However, there was a significant increase (p ≤ 0.05) in serum sodium and chloride levels of the treated and normal control groups, when compared with the diabetic control group respectively. The observed increase was consistent with treatment duration. The results therefore suggest that the antioxidant supplement might have a restorative effect on kidney function and also enhance effective electrolyte balance and control for easy movement of ions across cell membrane.

The Effect of Antioxidantson Electrolytes in Vancomycin- Streptozotocin InducedDiabetes Kidney Disease inRabbits.

“…9 There was a significant increase in serum (potassium, and chloride) and non-significant increase in serum sodium in treated groups compared with diabetic control group.Some studies show the role of antioxidants in improvement the serum potassium level in diabetic kidney disease like use combination of vitamins A, vitamin C, α-lipoic acid and other antioxidant supplement that increase of serum potassium level in diabetic treated group. 7 Other study shows the effect of sweet potato extract indecrease of serum sodium level because of its constituents of antioxidants like flavonoids, tannins and others. 10 The effect of antioxidant supplement in diabetic kidney dysfunction is decrease oxidative stress and restore the electrolyte levels that include serum chloride and another electrolyte.…”