Antioxidants and sperm DNA damage: a clinical perspective

Zini, Armand; Gabriel, Maria San; Baazeem, Abdulaziz

doi:10.1007/s10815-009-9343-5

Cited by 178 publications

(122 citation statements)

References 71 publications

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“…However, it is believed that seminal oxidative stress could be one of the main factors in the pathogenesis of this condition (7). In addition, it is reported that 25% of infertile men have high levels of seminal ROS, which could cause lipid peroxidation, loss of motility, and DNA sperm damage (19,20). Because spermatozoa possess high amounts of polyunsaturated fatty acids in their plasma membranes, they are predisposed to oxidative injury.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Fazeli¹,

Salimi²

2016

Avicenna J Med Biochem

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Background: Oxidative stress is the result of an imbalance between the production and scavenging of reactive oxygen species (ROS). Recently, oxidative stress has been introduced as a major cause of male infertility. Objectives: The aim of the present study was to determine the correlation between total antioxidant capacity (TAC) and malondialdehyde (MDA) as markers of oxidative stress in relation to idiopathic male infertility and sperm parameters. Patients and Methods: This case control study was conducted using 35 men with idiopathic infertility and 34 men with proven fertility. Seminal plasma TAC and MDA were measured by ferric reducing ability of plasma (FRAP) and thiobarbituric acid (TBA) reaction methods, respectively. Results: Seminal TAC levels were significantly lower and seminal MDA levels were significantly higher in men with idiopathic infertility than in fertile men (P < 0.0001 and P = 0.004, respectively). A positive correlation was shown between sperm motility, sperm morphology, and TAC levels in men with idiopathic infertility (P = 0.002 and P = 0.002, respectively). In addition, there was a correlation between sperm motility and TAC levels in fertile men (P = 0.005). There was no correlation between sperm count and TAC levels in either men with idiopathic infertility or in fertile men. Negative correlations were observed between MDA levels and sperm motility, morphology, and sperm count only in men with idiopathic infertility (P = 0.003, P = 0.001, and P = 0.006, respectively). Conclusions: Our results show that oxidative stress could play an important role in male infertility as well as in sperm motility and sperm morphology.

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Section: Discussionmentioning

confidence: 99%

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Fazeli¹,

Salimi²

2016

Avicenna J Med Biochem

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“…Several studies have examined antioxidative compounds that can protect sperm DNA from cryopreservation and thawing injuries. 13,77 The cryopreservation-induced damage of the sperm membrane manifests as changes in the organisation and lipid composition of the membrane, leading to changes in sperm permeability. 78,79 Several reports on mammalian sperm have demonstrated the protective capacity of phospholipid-based cryoprotectants.…”

Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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“…Even if a few selected studies have shown that antioxidants have a positive effect on sperm characteristics, there still seems to be no definitive evidence that this therapy leads to higher pregnancy rates. [20][21][22][23][24][25][26][27] literature. Given the different antioxidants used (both alone and in combination), the different dosages, the in vivo or in vitro methodology and the different study end points, the following discussion should be considered alongside the data reported in Table 1.…”

Section: Introductionmentioning

confidence: 99%

The role of antioxidant therapy in the treatment of male infertility: an overview

Lombardo¹,

Sansone²,

Romanelli³

et al. 2011

Asian J Androl

100

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In recent years, many studies have focused on the effect of oxidative stress, reactive oxygen species (ROS) and antioxidants on the male eproductive system. Under physiological conditions, sperm produces small amounts of ROS, which are needed for fertilisation, acrosome reaction and capacitation. However, if an increased production of ROS is not associated with a similar increase in scavenging systems, peroxidative damage of the sperm plasma membrane and loss of DNA integrity typically occur, which leads to cell death and reduced fertility. Furthermore, since there is no linear correlation between sperm quality and pregnancy rates, an improvement in semen parameters should not be the sole outcome considered in studies of antioxidant therapies. A definitive conclusion regarding the benefit of these therapies is difficult to obtain, as most of the previous studies lacked control groups, considered different antioxidants in different combinations and doses, or did not evaluate pregnancy rates in previously infertile couples. Even if beneficial effects were reported in a few cases of male infertility, more multicentre, double-blind studies performed with the same criteria are necessary for an increased understanding of the effects of various antioxidants on fertility.

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Antioxidants and sperm DNA damage: a clinical perspective

Cited by 178 publications

References 71 publications

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

The role of antioxidant therapy in the treatment of male infertility: an overview

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