Antioxidants, Free Radicals Scavenging and Xanthine Oxidase Inhibitory Potentials of Ajuga iva L. Extracts.

Baghiani, Abderrahmane; Boumerfeg, Sabah; Adjadj, Moufida; Ameni, Djamila; Djermouni, Meriem; Khelifi-Touhami, Fatima; Charef, Noureddine; Khennouf, Seddik; Arrar, Lekhmici

doi:10.5530/ax.2011.4.5

Cited by 16 publications

(12 citation statements)

References 59 publications

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“…The oxidase form of the purified enzyme showed an activity of 1988.55 nmol of urate/min/mg protein. These results are similar to those reported by Baghiani et al 9,20,21 and Atmani et al 27 …”

Section: Statical Analysissupporting

confidence: 82%

“…These results agree with those reported in the literature. 21,26 The purified enzyme showed one major band of an approximate molecular weight of 150 KDa when run on SDS/PAGE ( Figure 1B). The oxidase form of the purified enzyme showed an activity of 1988.55 nmol of urate/min/mg protein.…”

Section: Statical Analysismentioning

confidence: 99%

“…The extraction was carried out using polar and non-polar solvents according to a procedure outlined by Baghiani et al 21 One hundred g of dried and powdered plant material was soaked in 1 L of 85 % aqueous methanol at 4°C for 16 h. Then, the residue obtained after filtration was re-extracted with 1 L of 50 % aqueous methanol for 4 h. The resulting solutions from the first and the second extractions were concentrated by a rotary evaporator to obtain the crude extract (CE). The CE was then fractioned with n-hexane, chloroform, and ethyl acetate to obtain these solvent extracts (HxE, ChE and EaE, respectively), the remaining aqueous extract was labeled AqE.…”

Section: Extraction Of Phenolic Compoundsmentioning

confidence: 99%

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Study of the Antioxidant Property and Xanthine Oxidase Inhibitory Activity of Various Extracts from the Algerian Medicinal Plant Paronychia argentea L.

Adjadj¹,

Djarmouni²

2018

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Introduction: Paronychia argentea L. is used in traditional medicine to treat various diseases in Algeria especially kidney stones. This study was focused on the antioxidant and xanthine oxidase inhibitory activities of these plant extracts. Methods: The different extracts were prepared using solvents of increasing polarity, and their total polyphenols and flavonoids contents were determined using spectrophotomet ric methods. The antioxidant activities of Paronychia argentea extracts (PAE) were assessed by their inhibitory effect on xanthine oxidase (XO) and their scavenger ability on superoxide radical (O 2•-) generated by this enzyme. Results: The analysis PAE showed that ethyl-acetate extract (EaE) has the highest concentration of total phenolics and flavonoids compared with the chloroform extract (ChE) and the crude extract (CE). Results revealed that all PAE were effective in both XO inhibiting and superoxide radical scavenging, with the following order for the two assays: ChE > EaE > CE. Conclusion: This study provided evidence that PAE had interesting antioxidant potential which was confirmed using two enzymatic methods. Therefore, this plant could be used to treat gout and lot of diseases, where inhibition of XO and scavenging of superoxide radical are necessary.

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Section: Statical Analysissupporting

confidence: 82%

Section: Statical Analysismentioning

confidence: 99%

Section: Extraction Of Phenolic Compoundsmentioning

confidence: 99%

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Study of the Antioxidant Property and Xanthine Oxidase Inhibitory Activity of Various Extracts from the Algerian Medicinal Plant Paronychia argentea L.

Adjadj¹,

Djarmouni²

2018

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“…XO was then eluted with 25 mM benzamidine with same buffer fractions of 2 mL. Samples were collected and their absorbance was measured at 280 nm …”

Section: Methodsmentioning

confidence: 99%

Potential cow milk xanthine oxidase inhibitory and antioxidant activity of selected phenolic acid derivatives

Nile

Keum

Nile

et al. 2017

J Biochem & Molecular Tox

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“…It also reflects the ability to inhibit the lipid peroxidation in vitro. 29,33 Burda and Oleszek. 34 reported that the differences in solubility of flavonoids, both aglycones, glycosides, and methoxylated derivatives, in a micellar water-lipid system may influence the results obtained from this test, and the partition of the compounds between the two phases can influence the oxidation results.…”

Section: Dpphmentioning

confidence: 99%

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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Antioxidants, Free Radicals Scavenging and Xanthine Oxidase Inhibitory Potentials of Ajuga iva L. Extracts.

Cited by 16 publications

References 59 publications

Study of the Antioxidant Property and Xanthine Oxidase Inhibitory Activity of Various Extracts from the Algerian Medicinal Plant Paronychia argentea L.

Study of the Antioxidant Property and Xanthine Oxidase Inhibitory Activity of Various Extracts from the Algerian Medicinal Plant Paronychia argentea L.

Potential cow milk xanthine oxidase inhibitory and antioxidant activity of selected phenolic acid derivatives

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

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