Antioxidative Action of Aspirin on Endothelial Function in Hypercholesterolaemic Rats

Abstract

The role of aspirin on vascular endothelial changes during hypercholesterolaemia prior to development of actual atherosclerotic lesions is not known. Therefore, in the present study, we tested the hypothesis that aspirin by its antioxidant action improves endothelial function in a rat model of hypercholesterolaemia. Hypercholesterolaemia was induced in Wistar rats by feeding a 1% cholesterol-rich diet for 10 weeks. Lipid profile, lipid peroxidation and reduced glutathione were estimated in serum. Endothelial function and β 2 -adrenoceptor activity was tested by studying the dose-response relationship of acetylcholine and isoproterenol, respectively, on isolated aortic tissues in an organ bath setup. Hypercholesterolaemic rats showed a significant increase in total cholesterol, low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C), and a significant fall in high-density lipoprotein cholesterol (HDL-C) compared to the control rats. Isolated aortic tissues from hypercholesterolaemic rats showed endothelial dysfunction and decreased sensitivity to β 2 -adrenoceptor. Treatment with aspirin was associated with a fall in total cholesterol, LDL-C and VLDL-C, and a significant rise in serum HDL-C. Aspirin treatment also restored endothelial function and β 2 -adrenoceptor activity. Hypercholesterolaemic rats showed free radical generation, evident by increase in serum lipid peroxidation and reduction in serum reduced glutathione content compared to the control rats. Aspirin treatment was associated with reduction in free radical stress evident by decreased lipid peroxidation and significantly prevented reduction in glutathione content compared to hypercholesterolaemic controls. Aspirin improves endothelial function and β 2 -adrenoceptor activity during experimentally induced hypercholesterolaemia in rats, possibly due to an antioxidant effect.

A time course study on prothrombotic parameters and their modulation by anti-platelet drugs in hyperlipidemic hamsters

“…In addition, associated parameters were also not evaluated in any single study conducted so far [3]. Spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats fed on HC diet up to 12 weeks did not exhibit impaired NOdependent vasodilation [5]; however, Wistar rats fed on HC diet for 10 weeks exhibited endothelial dysfunction [38]. In yet another study on SHR rats fed on HC diet for 1 to 6 months, endothelium dysfunction was observed after 3 months [8].…”

Nutritive effect of cabbage (<i>Brassica oleracea</i>) on growth, obesity, lipidaemia and haematology in broiler and pullet chickens

“…Synthetic products such as aspirin, ibrupofen, chloroquine, paracetamol, may be effective (Tauseef et al, 2008), but consumer preference trends now point to an increasing demand for products that are raised without synthetic drugs and/or additives. The continuous use of synthetic feed supplements stimulates growth in chickens, but the residue is often difficult to decompose and may cause health problems to the human consumers (Stoker, 2007).…”

“…At certain levels, synthetic products and drugs are known to be toxic and detrimental to health. Production related health problems and welfare issues pertaining to these synthetic products may include gastro-intestinal bleeding and gut leakage in high-dose aspirin, ibuprofen and acetamorphen administration (Balog and Hester, 1991;Cyrus et al, 2002;Tauseef et al, 2008;Al-Obaidi and Al-Shadeedi, 2010).…”

Antioxidative effect of aspirin on vascular function of aged ovariectomized rats

“…Napoli et al reported NOreleasing aspirin, but not aspirin, reduced vascular smooth muscle cell proliferation in response to vascular injury in old rats (Napoli et al 2002); although we have use the conventional form of aspirin, it was capable to achieved improvement of smooth muscle function. Similarly, it has been reported that ASP has ameliorating vasorelaxant effect to Ach in hypercholesterolemic rats (Tauseef et al 2008).…”

“…Whereas NSAIDs, namely ibuprofen and indomethacin (INDO) are the common cause of worsening in blood pressure (Sarafidis and Bakris 2008); especially INDO is used for the contractile treatment of patent ductus arteriosus (Lee et al 2012), another NSAID low-dose aspirin (ASP) is used as a longterm major treatment for prevention of cardiovascular disease. Most of the effects of ASP are attributed to its antithrombotic activity which is exerted by inhibition of cyclooxygenase-1 (Taubert et al 2004;Tauseef et al 2008). The direct antilipolytic effect on adipocytes (Tauseef et al 2008), the direct action of ASP on insulin secretion (Sethi et al 2011;Tauseef et al 2008), elimination of oxidative stress associated with inflammation (Wu et al 2002), ameliorating effect of endothelium-dependant arterial relaxation by increasing vasodilator NO release (Taubert et al 2004), prevention of atherosclerosis by inhibiting the vascular smooth muscle cell proliferation, and reducing the proinflammatory mediators (Tauseef et al 2008) have been reported as well.…”