Antioxidative Activity of Natural Isorhapontigenin

Abstract

ABSTRACT-Isorhapontigenin (ISOR), isolated from Belamcanda chinensis, is a derivative of stilbene. Its chemical structure is very similar to that of resveratrol, with a potent antioxidative effect. In the present study, we investigated the antioxidative activity of ISOR in vitro. Oxidative damage of rat liver microsomes, brain mitochondria and synaptosomes was induced by Fe 2+ -Cys, VitC-ADP-Fe 2+ and H2O2, respectively. The formation of malondialdehyde (MDA), decrease of reduced glutathione (GSH) and increase of ultra-weak chemiluminescence during the lipid peroxidation process were determined. In addition, the characteristic ultra-weak chemiluminescence of oxidative DNA damage induced by CuSO 4-Phen-VitC-H2O2 system was studied. The results showed that ISOR significantly inhibited MDA formation in liver microsomes, brain mitochondria and synaptosomes induced by Fe 2+-Cys. Also, ISOR markedly prevented the decrease of GSH in mitochondria and synaptosomes induced by H2O2 and the increase of ultra-weak chemiluminescence during lipid peroxidation induced by VitC-ADP-Fe 2+ as well as oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2. The effects of ISOR at 10 -5 and 10 -6 mol/ L on the MDA formation and decrease of GSH were similar to that of the classical antioxidant vitamin E (10 -4 mol/ L). It may be concluded that ISOR possessed potent antioxidative activity and was much more potent than vitamin E.

Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats

“…4). In a study by Wang et al [41], they reported that the inhibition of MDA formation by ISOR is about 100 times more potent than that of the classical anti-oxidant vitamin E at the same concentration. Also, in a recent study, Wang et al [40] found that REV and its analogue ISOR may effectively upregulate the expression of SOD2 and GPX1 in hematopoietic cells following ionizing radiation-induced injury.…”

“…Group I included normal rats that received vehicle (2 ml of physiological saline) by intraperitoneal injection on two consecutive days with an interval of 24 h between applications. Group II included normal rats administered ISOR (10 −6 mol/L, in drinking water) [41] for 30 days. Group III included normal rats administered n-3 PUFA (0.3 g/kg/day by gavage) [8] for 30 days.…”

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

“…In response to these, I. obliquus has evolved a complex series of integrated defense systems. In recent years, more than 20 different kinds of bioactive components have been found in I. obliquus mainly including inooidiol the precursor of vitamin D2, betulinic acid, oxygenated triterpenes (Handa et al, 2010), superoxide dismutase (SOD), trametenolic acid, dextran, tannin compounds, steroids, alkaloid, lanosterol (Nakamura et al, 2009), lanoline alkane type three terpenes, lignin, melanin catechol (Zheng et al, 2009), folic acid derivatives, sheath ammonia fat analogs, mannitol, polyphenol, lanosterol (Wang et al, 2001), half the tannin compounds, aromatic substances (Zhao and Piao, 2006;Taji et al, 2008), β-glucan (Rhee et al, 2008), peptides (Hyun et al, 2006), polysaccharides (Cui et al, 2005;Zhang et al, 2007;Nakajima et al, 2007), polyphenols (Cui et al, 2005;Lee et al, 2007), triterpenoids, and steroids (Cui et al, 2005). Some components and their effects on tumor cell can be found in Table 1.…”

Neuronal Mitochondrial Toxicity of Malondialdehyde: Inhibitory Effects on Respiratory Function and Enzyme Activities in Rat Brain Mitochondria

“…The process of ischemiareperfusion increased rat brain MDA levels to 5.5 nmol/mg from 3.5 nmol/mg in controls [18]. Wang et al [19] reported that the MDA concentration in brain cortex mitochondria in rat was 8.38 nmol/mg, which was increased to 24.63 nmol/mg by exposure to Fe 2? -Cys.…”