Antioxidative and antigenotoxic effect of vitamin E against patulin cytotoxicity and genotoxicity in HepG2 cells

Abstract

Patulin (PAT) is a mycotoxin produced in fruits, mainly in apples, by certain species of Penicillium, Aspergillus, and Byssochlamys. It has been shown that PAT is cytotoxic, genotoxic, and mutagenic in different cell types. Several studies incriminate the oxidative stress as a mechanism of PAT-mediated toxicity. In this context, our aim was to investigate the protective role of Vitamin E (Vit E), an antioxidant agent, against PAT induced cytotoxicity and genotoxicity in cultured HepG2 cells. The obtained results showed that addition of Vit E in cells treated with PAT significantly reduce cell mortality induced by this toxin. In the same conditions, Vit E decreased the intracellular level of ROS, reduced PAT induced p53 expression, and reversed PAT induced DNA damage. In addition, Vit E prevented significantly the percentage of chromosome aberrations induced by PAT in HepG2 cells in a concentration dependant manner. These results suggest that Vit E, an exogenous antioxidant agent, plays an important role in defense against PAT-induced cytotoxicity and genotoxicity, which confirms the involvement of oxidative stress in the induction of DNA damage by PAT in HepG2 cells.

Genotoxicity assessment in iron deficiency anemia patients using sister chromatid exchanges and chromosomal aberrations assays

“…Vitamin E is a group of fatsoluble compounds, including d-alpha-tocopherol, which has strong antioxidant activity [44]. Our result is consistent with previous studies that demonstrated the protective effects of vitamin E against the genotoxicity of several agents such as deltamethrin and patulin [45,46]. Thus, vitamin E has the potential to be used as a protective drug against the genotoxicity associated with mutagenic agents and diseases.…”

Crocin Prevents Patulin‐Induced Acute Toxicity in Cardiac Tissues via the Regulation of Oxidative Damage and Apoptosis

“…There are a number of research studies examining the toxicity of PAT in mammalian cells. They demonstrated that PAT induces oxidative DNA damage, chromosome aberrations, and micronucleus formation [9][10][11][12][13]. Other investigators studied the in vivo administration of PAT and revealed that kidney, liver, intestinal tissue, and immune system as its target organs [14,15].…”

Genotoxic Effects of Mycotoxins: Review

“…149,154 PAT'nin indüklediği oksidatif stresi ve kromozomal anormalliklerini azaltmaya yönelik son yıllarda yapılan çalışmalarda, antioksidan ve antigenotoksik maddelerin bu olumsuz etkileri düzel-tebildiği gösterilmiştir. [155][156][157] ALTERNARİA TOKSİNLERİ Alternaria toksinleri, Alternaria cinsi özellikle de Alternaria alternata tarafından üretilen sekonder metabolitlerdir. Bu mikotoksinler bitkilerde birikerek gıda üretim zinciri içerisinde yer almaktadır.…”