Antioxidative and antihemolytic activities of soybean isoflavones

Abstract

Two properties of soybean isoflavones were examined in in vitro systems: (1) their antioxidative potency by measuring the extent of inhibition of lipoxygenase action and (2) their antihemolytic activity by measuring their ability to prevent peroxidative hemolysis of erythrocytes. The extent of the antioxidative capacity of isoflavones is positively correlated to the number of hydroxyl groups in the isoflavone nucleus. Glycosidation of isoflavones depressed their antioxidative activity considerably. A different susceptibility of erythrocytes of sheep, rats, and rabbits to the antihemolytic activity of isoflavones was found. Very high antihemolytic activity of some isoflavones such as daidzein was exerted toward sheep erythrocytes, very little and only by genistein was exerted toward rat erythrocytes, and no antihemolytic effect was noted at all on rabbit erythrocytes. A hemolysis enhancing activity of small amounts of isoflavones on rabbit erythrocytes, but not on those of sheep and rats, has been observed. The antioxidative and antihemolytic activities of isoflavones toward sheep erythrocytes were differently affected by structural differences of isoflavones.

Japanese Soybean Paste Miso Scavenges Free Radicals and Inhibits Lipid Peroxidation.

“…Vitamin E, a major lipid-soluble chain-breaking antioxidant, has been demonstrated to impart oxidative stability and antioxidant activity in soybean oil (17). Isofla vones from soybeans (18) have been found to have antioxidant action (1). Oral administration of saponins in rats prevented liver injury and hyperlipemia induced by high fat diet containing highly peroxidized corn oil-indicative of saponins' protective action in vivo and in vitro production of lipid peroxides (19).…”

Effects of processing conditions on the stability of polyphenolic contents and antioxidant capacity of Dolichos lablab L.

“…Antihemolytic activity Antihemolytic activity of the extracts was assessed as described by Naim et al (1976). The erythrocytes from cow blood were separated by centrifugation and washed with phosphate buffer (pH 7.4).…”

Antihemolytic and antioxidant activities of Allium paradoxum

“…Antihemolytic activity of the extracts was assessed as described by Naim et al [29,30] with a slight modification. Erythrocytes from male Wistar rat blood were separated by centrifugation and washed with phosphate buffer (pH 7.4), and diluted with phosphate buffered saline to give a 4% suspension.…”