Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate–salt hypertensive rats

Athiroh, Nour; Permatasari, Nur; Sargowo, Djanggan; Widodo, M. Aris

doi:10.1016/j.bgm.2014.01.001

Cited by 17 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Maceration was repeated three times and followed by evaporation. The samples were then labeled and stored in the freezer (Eno et al, 2004;Kawamura and Pamudji, 2005;Mustofa et al, 2013;Athiroh et al, 2014a;BPOM, 2014).…”

Section: Extraction Of Scurrula Atropurpurea Leavesmentioning

confidence: 99%

“…The tube then centrifuged at 3000 rpm and the supernatant was collected. Phosphate buffer was added into the supernatant up to 3.3 mL and the mixture was then measured by UV spectrophotometer (1700 Pharma Sec) and UV-Vis spectrophotometer (Shimadzu) at 580 nm wavelength (Athiroh and Sulistyowati, 2013;Mustofa et al, 2013;Athiroh et al, 2014a).…”

Section: Sod Activity Analysismentioning

confidence: 99%

“…In previous in vivo research, tea parasite (S. atropurpurea Bl. Danser) able to improve superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) concentration in deoxycorticosterone acetate (DOCA)-salt induced hypertensive rat (Kawamura and Pamudji, 2005;Athiroh and Sulistyowati, 2013;Mustofa et al, 2013;Athiroh et al, 2014a). Thus, the tea parasite plant can reduce oxidative stress in hypertension model rat.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.)

Athiroh

Wahyuningsih

2017

J Kedokt Hewan

Self Cite

View full text Add to dashboard Cite

The aim of this research was to investigate the potential effect of methanolic extract of Scurrula atropurpurea (MESA) in reducing oxidative stress through observation of superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration in mice. In this research, 25 male mice were divided into four groups with five replications. Group I (K1, without MESA) was considered as a control group, while Group II (K2), III (K3), and IV (K4) were given 1000, 2000, and 4000 mg MESA/kg BW, respectively. After 14 days, SOD activity and MDA concentration were measured spectrophotometrically. The SOD activity did not significantly increased (P>0.05) in group K1, K2, K3, and K4 with values were 19.469±2.072, 17.4±1.373, 20.53±3.411, and 23.87±2.528 U/ml, respectively. In contrast, MDA concentration was decreased (P>0.05) with values of 492.750±19.418, 492.750±19.418, 457.125±30.095, 454.625±19.080, and 475.875±37.659 ng/mL in groups K1, K2, K3, and K4, respectively. It can be concluded that the administration of MESA tend to increase SOD activity and decrease MDA concentration in this study, thus might be able to reduce oxidative stress in mice. ____________________________________________________________________________________________________________________ Key words: acute toxicity, MESA, mice, oxidative stress ABSTRAK Penelitian ini bertujuan mengetahui pengaruh penambahan laktosa pada berbagai konsentrasi dalam pengencer Tris kuning telur terhadap kualPenelitian ini bertujuan mengetahui efek ekstrak Scurrula atropurpurea (MESA) terhadap penurunan stres oksidatif melalui observasi aktivitas superoxide dismutase (SOD) dan konsentrasi malondialdehyde (MDA) pada mencit. Dalam penelitian ini digunakan 25 mencit jantan yang dibagi ke dalam empat kelompok dengan lima ulangan. Kelompok I (K1, tanpa MESA) merupakan kelompok kontrol, sedangkan Kelompok II (K2), III (K3), dan IV (K4) masing-masing diberikan 1.000, 2.000, dan 4.000 mg MESA/kg bobot badan. Setelah 14 hari, aktivitas SOD dan konsentrasi MDA diukur menggunakan spektrofotometri. Aktivitas SOD meningkat secara tidak signifikan (P>0,05) pada K1; K2; K3;469±2,072; 17,4±1,373; 20,53±3,411, dan 23,87±2,528 U/ml. Sebaliknya,750±19,418; 492,750±19,418; 457,125±30,095; 454,625±19,080; dan 475,875±37,659 ng/ml pada K1; K2; K3; dan K4. Dapat disimpulkan bahwa pemberian MESA cenderung meningkatkan aktivitas SOD dan menurunkan konsentrasi MDA sehingga diduga mampu mengurangi stress oksidatif pada mencit.litas spermatozoa ____________________________________________________________________________________________________________________ Kata kunci: toksisitas akut, MESA, mencit, stres oksidatifperanakan Etawa, laktosa, preservasi,

show abstract

Section: Extraction Of Scurrula Atropurpurea Leavesmentioning

confidence: 99%

Section: Sod Activity Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.)

Athiroh

Wahyuningsih

2017

J Kedokt Hewan

Self Cite

View full text Add to dashboard Cite

show abstract

“…As mentioned above, the observed results may be due to the high polyphenolic content of the plant extract. Accordingly, a lower level of oxidative stress and improvement in antioxidant status was found in many types of research after polyphenol-rich food administration mainly based on the reduction of thiobarbituric acid reactive substance levels (TBARS) [50,51]. According to Soma Gupta et al [52] it was shown that a relationship existed between the administration of a supplement rich in polyphenols and decreased MDA levels.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Antioxidant Activity Assessment of Tamus Communis L. Roots

Zerargui

Baghiani

Khennouf

et al. 2016

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: The aim of this study was to evaluate the antioxidant activities of Tamus communis L. (T. communis) roots. This plant is used in folk medicine in the treatment of rheumatism, arthritis and lumbago. Methods:The extraction of polyphenols has been followed by the determination of total polyphenols and flavonoids. The methanol extract (MeOHE) was chromatographied on a silica gel column. The column was eluted with chloroform and then with chloroform/methanol mixtures of increasing polarity, 6 main fractions (FI--FVI)) were collected and grouped according to their TLC (Thin layer chromatography). Several methods were used to evaluate the in vitro antioxidant activity such as DPPH (2, 2'-diphenyl-1-picrylhydrazy), bleaching of β-carotene for 24 h, lipid peroxidation and anti-hemolytic activity. An in vivo approach was carried out on mice treated with CE (crud extract) at a dose of 100 mg/Kg/day for 21 consecutive days, and one group was treated with vitamin C (Vit C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Results:The results showed that the highest level of polyphenols was recorded in FII (73.143±0.009 mg GA-Eq/g extract (Gallic Acid Equivalent))/g of extract) followed by MeOHE (69.786±0.10 mg GA-Eq/g extract). Total flavonoid contents of extracts/fractions showed that MeOHE contains the highest level. Inhibition of DPPH radical showed that MeOHE (0.1187±0.025 mg/ml) and CE (0.2363±0.019 mg/ml) have the most efficient antioxidant activity. All the extracts were capable of inhibiting the bleaching of β-carotene by scavenging linoleate derived free radicals, and all the studied extracts have a very important antihemolytic activity (p<0.001) and revealed an extension of hemolysis half time from 70.34±2.15 min of CTL (AAPH (2,2-azo-bis (2-amidinopropane) dihydrochloride)) and 207±5.65 min for FI. The in vivo assay was exhibited a significant decrease (54.09%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (47.30% and 46.87% respectively). Conclusion:These findings suggest that the extract obtained from T. communis have active substances contributing to the increase in natural antioxidant potential.

show abstract

“…Hypertension is one of the important risk factors for the cardiovascular diseases and is associated mostly with coronary heart disease, renal disease and cerebrovascular disease. Therefore, it is a major cause of morbidity and mortality (Athiroh et al, 2014). The recent studies have predicted that the prevalence of hypertension, at global level, will increase from 1 billion of the adults to 1.5 billion in the year 2025 (Gutiérreza et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-oxidant, hypotensive and diuretic activities of Origanum glandulosum in rat

Bouaziz

Khennouf

Zarga

et al. 2019

Bangladesh J Pharmacol

View full text Add to dashboard Cite

In vitro anti-oxidant, hypotensive and diuretic activities of Origanum glandulosum in rat BJP Thus, much attention has been paid to the study of medicinal plants, including the screening of natural bioactive compounds such as phenolic compounds with the ability to cure, prevent the progression of many diseases (Yadegarinia et al., 2006) and the exploration of traditional medicines may reveal new treatment options to increase the therapeutic arsenal by the discovery of novel medicines and to offer lower cost alternatives in developing countries that increasingly AbstractThe objective of this study was to evaluate the in vitro anti-oxidant, hypotensive and the diuretic activities of Origanum glandulosum leaves in rat. Chemical analysis revealed the isolation of katuranin and 5-isopropyl-3methylphenol. Ethyl acetate extract possessed highest scavenging activity against DPPH and hydroxyl radicals. Intravenous administration of extracts (0.04 to 12 mg/kg body weight) dose-dependently decreased the blood pressure (systolic, diastolic and mean) of the anesthetized rat. Methanol extract showed dose-dependent diuretic activity by increasing the urine output (77%) and the urinary excretion of sodium and potassium. In conclusion, this study supports the use of O. glansulosum in traditional medicine as hypotensive and diuretic agents. Article Info

show abstract

Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate–salt hypertensive rats

Cited by 17 publications

References 25 publications

STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.)

STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.)

Antioxidant Activity Assessment of Tamus Communis L. Roots

In vitro anti-oxidant, hypotensive and diuretic activities of Origanum glandulosum in rat

Contact Info

Product

Resources

About