This research aims to make macaroni from wheat flour, which is substituted with mocaf flour and purple yam flour. It is hoped that the macaroni produced will contain anthocyanin as a natural antioxidant. Macaroni made using wheat flour: mocaf flour (g:g), 10:60; 20:50; 30:40 and 10:70; 20:60 30:50 and 10:80; 20:70; 30:60 and purple yam flour treatment of 30; 20 and 10 g. The experimental design in this study was a completely randomized design with two replications; the parameters analyzed were antioxidant activity (DPPH), anthocyanin content, total phenol content, color, and preference of macaroni. The data obtained was subjected to a statistical test using the ANOVA method at a confidence level of 95%, and then the DNMRT test was carried out. The research showed that macaroni made with wheat flour, mocaf flour 20 g:50 g, and purple yam flour 30 g produced macaroni with high antioxidant activity (8.3% RSA) and was preferred. This research can reduce the use of flour in making macaroni. The characteristics of macaroni from this research are total phenolic content 52.20 mg GAE/100 gr (wb), anthocyanin content 16.39 mg/100 g (wb), color L* = 48.59, a*=3.62, b* = -0,99 and overall acceptance = 3.38 (more preferred).