Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

doi:10.5187/jast.2007.49.6.729

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Because it can specifically bind desired substances like cholesterol, PUFAs, proteins, metal ions and tumour cells, chitosan defends the cell by chelating action. Besides, Jang et al (2007) clarified that some antioxidants such as pyruvate, taurine and melatonin improved the reproductive ability of sperms. In addition, Lee et al (2017) found that curcumin improved the reproductive ability of sperm due to its antioxidant activity as well as Sameh et al (2020) suggested that curcumin nanoparticles (CUNPs) supplementation could improve the capability of seminal plasma to decrease the OS (Ogbuewu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

Mahmoud,

Abd‐Allah,

Abdel‐Halim

et al. 2023

Reprod Domestic Animals

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This study aimed to investigate the effect of supplementation of chitosan nanoparticles (CSNPs) on the capacitation of bovine spermatozoa during the in vitro fertilization process. Hyperactivated motility (HAM) and acrosome reaction (AR) of sperm cells as well as in vitro fertilization and cleavage rates are the main parameters used to estimate the effect of CSNPs on bovine spermatozoa's fertilizing ability. In this study, three different concentrations of CSNPs (10, 20 and 100 μg/mL) were prepared and characterized. Motile spermatozoa were separated from frozen–thawed semen by a swim‐up technique and capacitated in Sperm‐TALP medium supplemented with heparin only without CSNPs treatment (positive control), heparin + 10 μg/mL CSNPs, heparin + 20 μg/mL CSNPs, heparin + 100 μg/mL CSNPs and the last one served as a negative control tube which supplemented with 10 μg/mL CSNPs without adding heparin. Sperm cells were incubated for 90 min at 39°C in a 5% CO2 incubator and evaluated every 30 min at intervals. Cumulus oophorus complex (COCs) were matured in a 5% CO2 incubator at 39°C and inseminated in vitro with frozen–thawed bull sperm of the above concentrations. The inseminated oocytes were incubated at 39°C in a 5% CO2 incubator for 24 h and then examined for evidence of fertilization. The results of this investigation showed that HAM and AR were best affected by CSNPs at a concentration of 20 μg/mL during an incubation time of 60 min. As time went on, the overall proportion of spermatozoa with progressive motility (PM) decreased across all groups, and a substantially lower value was found at the dose mentioned above. Additionally, the impact of sperm treated with CSNPs on fertilization rate was assessed. The outcomes demonstrated that in comparison to the other concentrations (10 and 100 μg/mL), the positive control and the negative control, the proportion of fertilized oocytes was significantly higher in the CSNPs concentration (20 μg/mL). In conclusion, it could be inferred from this investigation that CSNPs support sperm functions during IVF and can be used for biomedical interventions in bovine spermatozoa. Additionally, a high IVF rate was achieved by using sperm treated with CSNPs as CSNPs enhance sperm capacitation and acrosome reaction.

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Section: Discussionmentioning

confidence: 99%

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

Mahmoud,

Abd‐Allah,

Abdel‐Halim

et al. 2023

Reprod Domestic Animals

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Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation

Hong

Sim

Park

et al. 2018

J Anim Reprod Biotechnol

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Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA (chitosan+hyaluronic acid) and GnHG (chitosan hydrogel) as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL), respectively. Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. CASA analysis showed GnHA and GnHG are effective against cryopreserved boar sperm. And antioxidant effect is measured by lipid peroxidation analysis. GnHA and GnHG, which is chitosan complex are effective for boar sperm cryopreservation by antioxidant effect.

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Melatonin supplementation improves the intactness of plasma membrane and acrosomal membrane of cryopreserved spermatozoa in Hariana bull

YADAV

Kumar

Saxena

et al. 2019

Indian J of Anim Sci

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Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

Cited by 3 publications

References 20 publications

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

Ameliorative effect of chitosan nanoparticles in capacitation media on post‐thawing in vitro fertilizing ability of bovine spermatozoa

Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation

Melatonin supplementation improves the intactness of plasma membrane and acrosomal membrane of cryopreserved spermatozoa in Hariana bull

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