Antioxidative effect of BHA in soya bean lecithin‐based extender containing Glycerol or DMSO on freezing capacity of goat semen

Abstract

The aim of this study was to evaluate the effects of butylated hydroxyanisole (0 or 4 mM) along with different concentrations (5 or 7%) of glycerol (G) and dimethyl sulphoxide (DMSO) as cryoprotectant (CPAs) on freezability of goat semen. Semen was collected from four bucks (3-4 years) twice a week for five weeks. The pooled ejaculates were diluted with extender containing two different concentrations of G or DMSO in combination with BHA. Afterwards, the diluted samples were loaded into 0.25 ml straws and frozen using a standard protocol. After thawing motility parameters, viability, membrane integrity and total abnormality were assessed. The Results showed that the presence of BHA in extender, type and level of CPAs as main factors had significant effects on goat sperm viability, total and progressive motility after freezing-thawing processes (p < .05). Also, the interaction of BHA (0 and 4 mM) and levels of G or DMSO (5 or 7%) had a significant effects (p < .05) on total motility, viability and some characteristic. In this case, the addition of 5% G or DMSO with BHA resulted in highest motility and viability than the other groups (p < .05). The addition of G5 (with and without BHA) increased VSL and reduced abnormality than the other groups (p < .05). The results showed that the main effects of CPAs and CPAs level on membrane functionality were significant (p < .05). Also there were no significance differences in the interactive effects of MDA, VCL, VAP, ALH, LIN and STR among the groups (p > .05). Finally, it can be concluded that the use of 5% CPAs with or without BHA may result in better post-thaw sperm quality of goat.

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

“…Contextually, broad evidence suggests higher concentrations may not necessarily translate into better quality, but indeed may be detrimental [55]. In agreement with the present study, the use of other antioxidants such as arbutin, butylated hydroxyanisole, rosemary or lycopene, have reported a positive effect on goat sperm motility [11,44,45,56].…”

“…Based on the current results, the addition of HT at low concentrations (10 µg/mL) may improve membrane integrity, showing an increase of 14% compared to Control treatment, as reported by Hamden et al [22] when HT was added. Similarly, some authors reported an increase of membrane integrity when other antioxidants were added in extender medium [8,56,58,59].…”

Influence of various dietary fat sources on freezing capacity of Moghani ram semen

“…a Each kg of the vitamin-mineral premix contained: vitamin A: 50 000 IU; vitamin D3: 10 000 IU; vitamin E: 0.1 g; calcium: 196 g; phosphorus: 96 g; sodium: 71 g; magnesium: 19 g; iron 3 g; copper: 0.3 g; manganese: 2 g; zinc: 3 g; cobalt: 0.1 g; iodine: 0.1 g; selenium: 0.001 g The hypo-osmotic swelling test (HOST) was used to assess the functional functionality (MF) of the sperm membrane. The HOST was performed by incubating 30 µL semen with 300 µL of a 100 mOsm hypoosmotic solution (0.9 g fructose and 0.49 g sodium citrate dissolved in 100 mL distilled water) at 37 °C for 30 min (Rahmatzadeh et al, 2017). After incubation, 10 µL of the mixture was placed on a glass slide and covered with a cover slip to evaluate under a phase-contrast microscope (×400).…”

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

“…DMSO is a cryoprotectant that quickly enters sperm cells. It can be used to maintain the frozen sperm quality of bulls [115], boars [29], goats [116], and dogs [117]. In stallions, it is preferable to use DMSO or methyl formamide alone or combined with glycerol in skim milk extenders as cryoprotectants and alternatives to extenders containing glycerol only [118,119].…”