Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress

Yuan, Meng; Zhang, Liang; Wang, Linquan; Zhou, Chunju; Shangguan, Yuxian; Yang, Yang

doi:10.1016/j.ecoenv.2019.02.026

Cited by 48 publications

(23 citation statements)

References 67 publications

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“…As product of PCS gene is being synthesized in high amount, lower GSH level in transformed hairy roots was probably because of the overconsumption for PCs synthesis and not due to its inhibition. The result nds support with Samane et al (2007), Ben Ammar et al (2008 and Meng et al (2019) who have found lower GSH but higher PCs level in heavy metal stressed Arabidopsis thaliana, tomato and lettuce respectively.…”

Section: Discussionsupporting

confidence: 90%

Heterologous Expression of Cyanobacterial PCS Confers Augmented Arsenic And Cadmium Stress Tolerance and Higher Artemisinin In Artemisia Annua Hairy Roots.

Pandey

Kumar

et al. 2021

Preprint

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The present study provides the first report of heterologous expression of phytochelatin synthase from Anabaena PCC 7120 (anaPCS) into the hairy roots of Artemisia annua. Transformed hairy roots of A. annua expressing anaPCS gene showed better tolerance to heavy metals viz., arsenic (As) and cadmium (Cd) owing to 143 and 191 % more As and Cd accumulation respectively as compared to normal roots with a bioconcentration factor (BCF) of 9.7 and 21.1 for As and Cd respectively. Under As and Cd stresses, transformed hairy roots possessed significantly higher amounts of phytochelatins and thiols probably due to the presence of both AaPCS (Artemisia annua PCS) and anaPCS. In addition, artemisinin synthesis was also induced in transformed hairy roots under heavy metals stresses. In-silico analysis revealed the presence of conserved motifs in both AaPCS and anaPCS sequences as well as structural modelling of PCS functional domain was conducted. Interaction of AaPCS and anaPCS proteins with CdCl2 and sodium arsenate gene ontology analysis gave insights to anaPCS functioning in transformed hairy roots of A. annua. The study provides transformed hairy roots of A. annua as an efficient tool for effective phytoremediation with added advantages of artemisinin extraction from hairy roots used for phytoremediation.

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Section: Discussionsupporting

confidence: 90%

Heterologous Expression of Cyanobacterial PCS Confers Augmented Arsenic And Cadmium Stress Tolerance and Higher Artemisinin In Artemisia Annua Hairy Roots.

Pandey

Kumar

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…As product of PCS gene is being synthesized in high amount, lower GSH level in transformed hairy roots was probably because of the overconsumption for PCs synthesis and not due to its inhibition. The result finds support with Samane et al ( 2007 ), Ben Ammar et al ( 2008 ) and Meng et al ( 2019 ) who have found lower GSH but higher PCs level in heavy metal stressed Arabidopsis thaliana , tomato, and lettuce, respectively.…”

Section: Discussionsupporting

confidence: 86%

Heterologous expression of cyanobacterial PCS confers augmented arsenic and cadmium stress tolerance and higher artemisinin in Artemisia annua hairy roots

Pandey

Kumar

2021

Plant Biotechnol Rep

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The present study provides the first report of heterologous expression of phytochelatin synthase from Anabaena PCC 7120 ( anaPCS ) into the hairy roots of Artemisia annua . Transformed hairy roots of A. annua expressing anaPCS gene showed better tolerance to heavy metals, viz., arsenic (As) and cadmium (Cd) owing to 143 and 191% more As- and Cd-accumulation, respectively, as compared to normal roots with a bioconcentration factor (BCF) of 9.7 and 21.1 for As and Cd, respectively. Under As and Cd stresses, transformed hairy roots possessed significantly higher amounts of phytochelatins and thiols probably due to the presence of both AaPCS ( Artemisia annua PCS ) and anaPCS . In addition, artemisinin synthesis was also induced in transformed hairy roots under heavy metals stresses. In-silico analysis revealed the presence of conserved motifs in both AaPCS and anaPCS sequences as well as structural modelling of PCS functional domain was conducted. Interaction of AaPCS and anaPCS proteins with CdCl 2 and sodium arsenate gene ontology analysis gave insights to anaPCS functioning in transformed hairy roots of A. annua . The study provides transformed hairy roots of A. annua as an efficient tool for effective phytoremediation with added advantages of artemisinin extraction from hairy roots used for phytoremediation. Supplementary Information The online version contains supplementary material available at 10.1007/s11816-021-00682-5.

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“…In order to avoid cells and tissues from ROS oxidative damage, plants will enhance the function of antioxidant system to remove excessive ROS. When HMs exposure such as Cd or Zn disturbs the metabolism balance of ROS, plants can correspondingly improve the function of the antioxidant system to eliminate excessive ROS (Afef 2019;Meng et al 2019). Khanna et al (2019) recorded Cd exposure with a concentration of 0.4 mM to result in the accumulation of O 2…”

Section: •-mentioning

confidence: 99%

The role of antioxidant mechanism in photosynthesis under heavy metals Cd or Zn exposure in tobacco leaves

Yang

Wang

He³

et al. 2021

Journal of Plant Interactions

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We examined the effects of Cd and Zn exposure on the photosynthetic function and the tolerant mechanisms of ROS metabolism in tobacco leaves. The results showed that the photosynthesis inhibition caused by Cd exposure was due to the limitation of both stomatal and non-stomatal factors, while Zn exposure only showed a significant effect on the Gs of tobacco leaves. Cd increased the generation rate of O 2 •and the content of H 2 O 2 , but Zn did not lead to the ROS burst. Cd enhanced the POD activity, but inhibited SOD and CAT activities. The activities of SOD and POD significantly increased under Zn exposure. Cd inhibited the activity and expression of APX. The up regulation of GPX, GR and GST expression and the increase of their activities also play a positive role in the adaptation to Cd. Although Zn inhibited the activities of APX and GPX, it had little effect on other enzyme activities and protein expression in AsA-GSH cycle. TPX and TrxR activities, Trx-Prx pathway-related proteins are very sensitive to Cd. However, only PrxQ and 2-Cys Prx BAS1, which are located in chloroplast, and Trx-HFC164 were significantly down-regulated in Trx-Prx pathway under Zn exposure.

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Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress

Cited by 48 publications

References 67 publications

Heterologous Expression of Cyanobacterial PCS Confers Augmented Arsenic And Cadmium Stress Tolerance and Higher Artemisinin In Artemisia Annua Hairy Roots.

Heterologous Expression of Cyanobacterial PCS Confers Augmented Arsenic And Cadmium Stress Tolerance and Higher Artemisinin In Artemisia Annua Hairy Roots.

Heterologous expression of cyanobacterial PCS confers augmented arsenic and cadmium stress tolerance and higher artemisinin in Artemisia annua hairy roots

The role of antioxidant mechanism in photosynthesis under heavy metals Cd or Zn exposure in tobacco leaves

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