Antiparasitic activity of plumericin & isoplumericin isolated from Plumeria bicolor against Leishmania donovani

Abstract

Background & objectives:The severe toxicity, exorbitant cost and emerging resistance of Leishmania species against most of the currently used drugs underscores the urgent need for the alternative drugs. The present study evaluates in vitro anti-leishmanial activity of Plumeria bicolor and its isolated compounds.Methods:The in vitro anti-parasitic activity of chloroform extract of Plumeria bicolor, plumericin and isoplumericin were tested alongwith appropriate controls against promastigote and amastigote forms of Leishmania donovani using 96 well microtiter plate. The concentration used for assessing the anti-leishmanial activity of extract of Plumeria bicolor and both isolated compounds were 100 μg/ml and 15 μM, respectively. The viability of the cells was assessed by MTT assay. The cytotoxicity of these compounds was performed against J774G8 murine macrophage cells lines at the concentration of 30 μM.Results:The Plumeria bicolor extract showed activity with the IC50 of 21±2.2 and 14±1.6 μg/ml against promastigote and amastigote forms of L. donovani, respectively. Plumericin consistently showed high activity with the IC50 of 3.17±0.12 and 1.41±0.03 μM whereas isoplumericin showed the IC50 of 7.2±0.08 μM and 4.1±0.02 μM against promastigote and amastigote forms, respectively. Cytotoxic effect of the chloroform extract of P. bicolor, plumericin and isoplumericin was evaluated in murine macrophage (J774G8) model with CC50 value of 75±5.3 μg/ml, 20.6±0.5 and 24±0.7 μM, respectively.Interpretation & conclusions:Our results indicated that plumericin showed more potent activity than isoplumericin and might be a promising anti-leishmanial agent against L. donovani.

Evaluation of Antileishmanial Activity of Selected Brazilian Plants and Identification of the Active Principles

“…This compound, previously isolated from Himatanthus sucuuba , presented IC 50 values of 0.9 and 1.0  μ M against promastigote and amastigote forms of L. amazonensis [32] and IC 50 of 3.17 ± 0.12 and 1.41 ± 0.03  μ M against promastigotes and amastigotes of L. donovani , respectively [33]. …”

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G 0 /G 1 Arrest followed by Necrotic Cell Death in Leishmania donovani

“…and for immunomodulatory effects on macrophages (20). Similarly, the antileishmanial activities of terpenoid derivatives were tested against promastigotes and intracellular amastigotes form of L. donovani (21). In the present study, the antileishmanial activities of Ag NPs and TiO 2 NPs that were green synthesized using the aqueous leaf extract of E. prostrata were evaluated against promastigotes and intracellular amastigotes of L. donovani.…”

Evaluation of antimycobacterial, leishmanicidal and antibacterial activity of three medicinal orchids of Arunachal Pradesh, India

“…The absorbance was measured at 570 nm and DMSO (0.5%) was considered as control while, amphotericin B and miltefosine was used as reference. Each assay was performed in duplicate with three independent experiments [ 17 ].…”