Antiparasitic activity of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides

Abstract

Parasitoses are very common throughout the world, generating serious consequences for public health. Leishmaniosis and giardiasis are examples of fairly recurrent, but neglected diseases. Several higher plants have demonstrated promising activity against the parasites. The aim of this study was to evaluate the biological activity of extracts, fractions and isolated compounds from the leaves and stems of two Brazilian plants: Eugenia mattosii and Marlierea eugeniopsoides (Myrtaceae) against Leishmania and Giardia. XTT and the fluorimetric method were used to for this evaluation, respectively. Cytotoxicity was evaluated against HeLa cells. The results demonstrated that chloroform fractions of E. matosii and pinostrobin presented the most pronounced antiparasitic activity, with the CLF-stems being the most effective against Leishmania amazonensis and Leishmania braziliensis. Pinostrobin also presented activity against G. lamblia. Therefore, E. mattosii stems and pinostrobin may be considered possible targets for the continuity of studies against other parasites.

Exploring Antiparasitic Molecule Sources from Timber by-Product Industries—Leishmanicidal and Trypanocidal Compounds from Clathrotropis brunnea Amshoff

“…Pinostrobin was isolated from dichloromethane extracts from Eugenia matossi (Myrtaceae) and showed in vitro activity against L. amazonensis and L. braziliensis, with an EC 50 of 22.7 and 11.3 μg/ml, respectively in the latter parasite, similar to the value found by us (13.6 × 0.28 μg/ml), thus reconfirming the leishmanicidal activity observed (Vechi et al, 2020). It was also reported to be active in Lychnophora markgravii (Vernonieae, Asteraceae) against L. amazonensis amastigotes as well as the analog flavanone tectochrysin.…”

The Pharmacokinetics, Tissue Distribution, Metabolism, and Excretion of Pinostrobin in Rats: Ultra-High-Performance Liquid Chromatography Coupled With Linear Trap Quadrupole Orbitrap Mass Spectrometry Studies

“…It is found more abundantly in roots than other secondary metabolites, such as pinocembrin and pinosylvin (Wang et al, 2016). Pinostrobin is known to have various pharmacological activities (Patel et al, 2016), including antiulcer, anti-Helicobacter pylori (Bhamarapravati et al, 2006), antioxidant, antiinflammatory, anticancer (Jaudan et al, 2018;Jones and Gehler, 2020;Sopanaporn et al, 2020), antidiarrheal, antiviral (Wu et al, 2011), antimicrobial (Hernández Tasco et al, 2020), anti-Alzheimer's, antiprotozoal, antinociceptive (Déciga-Campos et al, 2017), antimutagenic, antiplatelet (Zhang et al, 2017), antiproliferative (Siekmann et al, 2013;Jadaun et al, 2017), antileukemic (Smolarz et al, 2005), antiosteoporotic (Gu et al, 2017), and antiparasitic properties (Vechi et al, 2020). Moreover, pinostrobin can protect the gastric mucosa by reducing the ulcer area and mucosal content and reducing or eliminating submucosal edema and leukocyte infiltration.…”

Antiparasitic Activity of Plants Extract against Gastrointestinal Nematodes and Rhipicephalus microplus

“…Live adult worms of Gastrothylax indicus died within 4 hours of treatment with ethanolic and aqueous extract of plants (Aggarwal et al 2016). Therefore, the results of these studies suggest that plants can be potential sources for preparation of novel antiparasitic drugs (Estevam et al 2017;Azadbakht et al 2020;Vechi et al 2021).…”