Antiplatelet activity of loureirin A by attenuating Akt phosphorylation: In vitro studies

Hao, Hong-Zhen; He, Ao-Di; Wang, Dao-Chun; Yin, Zhang; Zhou, Yajun; Li, Gang; Liang, Minglu; Da, Xing-Wen; Yao, Guang-Qiang; Xie, Wen; Xiang, Jizhou; Ming, Zhang-Yin

doi:10.1016/j.ejphar.2014.10.059

Cited by 28 publications

(10 citation statements)

References 26 publications

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“…In a closer inspection, another study showed that PI3Kα inhibition augments thrombopoietin-mediated platelet activation and thrombus formation through restricting thromboxane A2 synthesis and extracellular signal-regulated kinase (ERK) phosphorylation [ 38 ]. Additionally, loureirin, a flavonoid extracted from dragon’s blood (a deep red resin secreted from Dracaena ), has been shown to suppress platelet activation through PI3K/AKT signaling pathway inhibition [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

The probable role and therapeutic potential of the PI3K/AKT signaling pathway in SARS-CoV-2 induced coagulopathy

2022

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Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is associated with a high mortality rate. The majority of deaths in this disease are caused by ARDS (acute respiratory distress syndrome) followed by cytokine storm and coagulation complications. Although alterations in the level of the number of coagulation factors have been detected in samples from COVID-19 patients, the direct molecular mechanism which has been involved in this pathologic process has not been explored yet. The PI3K/AKT signaling pathway is an intracellular pathway which plays a central role in cell survival. Also, in recent years the association between this pathway and coagulopathies has been well clarified. Therefore, based on the evidence on over-activity of the PI3K/AKT signaling pathway in SARS-CoV-2 infection, in the current review, the probable role of this cellular pathway as a therapeutic target for the prevention of coagulation complications in patients with COVID-19 is discussed.

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Section: Introductionmentioning

confidence: 99%

The probable role and therapeutic potential of the PI3K/AKT signaling pathway in SARS-CoV-2 induced coagulopathy

2022

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“…The study conducted by Chen et al revealed that the tryptophan derivative CD-26 exerts an anti-thrombotic effect by inhibiting the PI3K/Akt pathway in platelets ( 9 ). Furthermore, the study conducted by Hao et al suggested that lutein A may inhibit platelet activation by inhibiting the PI3K/Akt pathway ( 21 ). Thus, the PI3K/AKt pathway is an important participant in the regulation of platelet activity.…”

Section: Discussionmentioning

confidence: 99%

Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism

et al. 2018

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The present study investigated the predictive value of the platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. From January 2014 to January 2016, 23 patients with postpartum deep vein thrombosis of lower extremity treated in the Department of Obstetrics of Beijing Chaoyang Hospital were as assigned as the observation group. At the same time, 25 cases with normal recovery were assigned as the control group. Blood samples were collected from all the subjects. The platelet aggregation rate was measured using a platelet aggregation apparatus. Plasma platelet activating factor (PAF) levels were measured by ELISA. The positive rate of platelet P-selectin (CD62p) and lysosomal membrane glycoprotein (CD63) was measured by flow cytometry. PI3K expression and AKt phosphorylation levels were measured by western blot analysis. The ROC curve was used to evaluate the value of the platelet aggregation rate in predicting postpartum deep vein thrombosis of lower extremity. The correlation between the platelet aggregation rate and PAF and PI3K/AKt expression was also analyzed. The cesarean section rate, platelet 5-min maximum aggregation rate, PAF level and the positive rate of CD62p and CD63 were significantly higher in the control than those in the observation group (P<0.05). Furthermore, the platelet aggregation rate was positively correlated with the expression of PAF, CD62p and CD63 (r=0.389, 0.451, and 0.452; all P<0.05). The platelet 5-min maximum aggregation rate for predicting postpartum deep vein thrombosis of lower extremity was reflected by the area under the ROC curve (AUC=0.797, P=0.000). The PI3Kp110β/β-actin and p-AKt/AKt ratio was significantly higher in the observation compared with the control group (P<0.05). In addition, the platelet aggregation rate was positively correlated with the expression of PI3K and phosphorylation level of AKt (r=0.441, 0.430; all P<0.05). The results suggested that platelet aggregation activity is elevated in postpartum deep vein thrombosis patients. It has a certain predictive value for the occurrence of postpartum deep vein thrombosis of lower extremity. Thuss, the PI3K/AKt signaling pathway may be one of the mechanisms of platelet aggregation.

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“…Many studies have demonstrated that polyphenolic compounds are abundant sources of anti‐platelet ingredients, including polyphenolic grape extract (de Lange et al, 2007), resveratrol (Shen et al, 2007) and flavonoid extracted (Hao et al, 2015). Some carbohydrates also have anti‐platelet activity, such as water‐soluble polysaccharide purified from the mycelia of Cordyceps sinensis (Lu et al, 2014) and monosaccharides from sorghum vinegar (Li, Yu, & Fan, 2014).…”

Section: Introductionmentioning

confidence: 99%

The in vitro bioavailability of anti‐platelet peptides in collagen hydrolysate from silver carp ( Hypophthalmichthys molitrix ) skin

Wang

Лі

2020

J Food Biochem

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Previous animal experiments indicated collagen hydrolysates (CHs) intake decreased platelet release indicators in plasma and highlight potential applications as healthcare supplements to combat cardiovascular disease. The oligopeptides (GPR, GPRG, and GPRGP) have anti‐platelet activities. However, it is still unclear whether they are bioactive compounds in CHs from silver carp skin. We investigated the bioavailability of oligopeptides using simulated gastrointestinal digestion and Caco‐2 model. Anti‐thrombotic activities, in vitro platelet aggregation and formation of platelet thrombus, were evaluated. They resisted gastrointestinal digestion and could be absorbed by Caco‐2. Oligopeptides inhibited platelet aggregation induced by adenosine diphosphate and thrombin with IC50 of 0.160, 0.283, 0.251 mg/ml and 0.714, 1.008, 0.917 mg/ml for GPR, GPRG, and GPRGP, respectively. Oligopeptides prolonged the time of platelet thrombus and inhibited coagulation cascades, but CHs performed no bleeding side effect. These results confirmed that oligopeptides could be used as bioactive compounds of dietary supplements for pre‐thrombotic to prevent thrombosis. Practical applications Oligopeptides, GPR, GPRG, and GPRGP, derived from silver carp (Hypophthalmichthys molitrix) skin collagen, performed anti‐thrombotic activities from their anti‐platelet aggregation and anticoagulation activities. But the collagen hydrolysates containing these peptides had no side effect of bleeding in the mice model. Furthermore, this study investigated the bioavailability of these three bioactive peptides by the Caco‐2 cells model. Thus, oligopeptides GPR, GPRG, and GPRGP are a potential index of bioactive compounds in the preparation of anti‐thrombotic functional foods or healthcare supplements for people at the pre‐thrombotic state.

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Antiplatelet activity of loureirin A by attenuating Akt phosphorylation: In vitro studies

Cited by 28 publications

References 26 publications

The probable role and therapeutic potential of the PI3K/AKT signaling pathway in SARS-CoV-2 induced coagulopathy

The probable role and therapeutic potential of the PI3K/AKT signaling pathway in SARS-CoV-2 induced coagulopathy

Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism

The in vitro bioavailability of anti‐platelet peptides in collagen hydrolysate from silver carp ( Hypophthalmichthys molitrix ) skin

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