Antiplatelet and Anticoagulant Activities of Angelica shikokiana Extract and Its Isolated Compounds

Abstract

Angelica shikokiana is a Japanese medicinal plant that is used traditionally in several ailments of cardiovascular diseases. However, there is no report regarding its anticoagulant or antiplatelet activities. So this study was designed to screen for such activities (anticoagulant by prothrombin time [PT], activated partial thromboplastin time, and thrombin time assays and antiplatelet activities against adenosine 5'-diphosphate [ADP] and arachidonic acid-induced platelet aggregations) for the methanol extract of the aerial part (Angelica methanol extract [AME]), its isolated coumarins, flavonoids, and flavonoid metabolites. The AME had potent anticoagulant and antiplatelet activities, and the flavonoid compounds were evidenced to be responsible for such activities. Among coumarins compounds, hyuganin C showed significant prolongation of only PT, while other coumarins were inactive. Similarly, hyuganin C and bergapten were the only active coumarins against ADP-induced platelet aggregation. Compared to the parent compounds, colonic metabolites of the flavonoids had similar anticoagulant and antiplatelet activities, while glucuronides showed sharp decreases in all studied activities. This is the first report showing that the medicinal plant A shikokiana has potent antiplatelet and anticoagulant activities.

Flavonoids as antiobesity agents: A review

“…Although recognized for their antioxidant activity, flavonoids have several other activities, including anti‐inflammatory, 69 vasorelaxant, 70 anticoagulant, 71 cardioprotective, 72 antidiabetic, 73–76 chemoprotective, 77 neuroprotective, 78 and antidepressant properties 79 . Furthermore, available data suggest that flavonoids from dietary and herbal plants possess potentially beneficial effects on the prevention and treatment of obesity, and its relation to metabolic disorders 80,81 .…”

Antithrombotic activity of flavonoids and polyphenols rich plant species

“…This was attributed to quercetin and myricetin. Angelica shikokiana Makino ex Y. Yabe, herbal extracts showed reduction in platelet aggregation at all analyzed concentrations (1 mg L -1 being the lowest, i.e., 3.3 µmol L -1 of quercetin equivalent) in platelet aggregation assays induced by ADP and arachidonic acid (44). This was attributed to polyphenols that were detected in the extract and individually tested in the same platelet assays.…”

Utilisation des feuilles de <i>Ficus sycomorus</i> L. (Moraceae) dans la prévention de l’hypercoagulation chez les drépanocytaires: identification de composés phénoliques potentiellement anticoagulant et antiagrégant plaquettaire

“…Le kaempferol-3-O-glucoside identifié dans l'espèce Angelica shikokiana (Apiaceae) et reconnu pour ses effets antiagrégant plaquettaire et anticoagulant (Mira et al, 2017) a la même masse m/z égale à 448, 10055 et la même formule brute C 21 H 20 O 11 que le composé 1 qui a élué à 0,53 min dans notre étude.…”

“…La Quercetine glucuronide isolée de l'espèce Angelica shikokiana (Mira et al, 2017) a la même masse m/z égale à 478, 07474 et la même formule chimique C 21 H 17 O 13 que le composé 5 qui a élué à 3, 11 min dans notre étude. Les composés 1 ; 4 et 5 agiraient comme la plupart de ces flavonols identifiés, par une inhibition de la formation de thromboxane A2, un fort inducteur de l'agrégation plaquettaire (Arslan et al, 2011).…”