Antiproliferation and apoptosis induced by curcumin in human ovarian cancer cells

Shi, Mingxin; Cai, Qiufeng; Yao, Luming; Mao, Yonghui; Ming, Yanlin; Ouyang, Gaoliang

doi:10.1016/j.cellbi.2005.10.024

Cited by 200 publications

(145 citation statements)

References 28 publications

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“…The concentration of surfactant was maintained at 0.1 M, which is above the CMC for each of the micelles, in nondeuterated and deuterated tris buffer. Two stock solutions of curcumin in methanol and methanol-d 4 were used for steady-state, time-correlated singlephoton counting (TCSPC) measurements. A small quantity (2 µL) of the curcumin stock solution (4.5 or 1.5 mM) was transferred to the micellar solution to yield a solution with a curcumin concentration of 3 or 1 µM for the UV-vis or fluorescence spectra, respectively.…”

Section: Methodsmentioning

confidence: 99%

Excited-State Intramolecular Hydrogen Atom Transfer of Curcumin in Surfactant Micelles

et al. 2010

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Femtosecond fluorescence upconversion experiments were performed on the naturally occurring medicinal pigment, curcumin, in anionic, cationic, and neutral micelles. In our studies, the micelles are composed of sodium dodecyl sulfate (SDS), dodecyl trimethyl ammonium bromide (DTAB), and triton X-100 (TX-100). We demonstrate that the excited-state kinetics of curcumin in micelles have a fast (3−8 ps) and slow (50−80 ps) component. While deuteration of curcumin has a negligible effect on the fast component, the slow component exhibits a pronounced isotope effect of ∼1.6, indicating that micelle-captured curcumin undergoes excited-state intramolecular hydrogen atom transfer. Studies of solvation dynamics of curcumin in a 10 ps time window reveal a fast component (≤300 fs) followed by a 8, 6, and 3 ps component in the solvation correlation function for the TX-100, DTAB, and SDS micelles, respectively. October 23, 2009; ReVised Manuscript ReceiVed: January 4, 2010 Femtosecond fluorescence upconversion experiments were performed on the naturally occurring medicinal pigment, curcumin, in anionic, cationic, and neutral micelles. In our studies, the micelles are composed of sodium dodecyl sulfate (SDS), dodecyl trimethyl ammonium bromide (DTAB), and triton X-100 (TX-100). We demonstrate that the excited-state kinetics of curcumin in micelles have a fast (3-8 ps) and slow (50-80 ps) component. While deuteration of curcumin has a negligible effect on the fast component, the slow component exhibits a pronounced isotope effect of ∼1.6, indicating that micelle-captured curcumin undergoes excited-state intramolecular hydrogen atom transfer. Studies of solvation dynamics of curcumin in a 10 ps time window reveal a fast component (e300 fs) followed by a 8, 6, and 3 ps component in the solvation correlation function for the TX-100, DTAB, and SDS micelles, respectively. Keywords

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Section: Methodsmentioning

confidence: 99%

Excited-State Intramolecular Hydrogen Atom Transfer of Curcumin in Surfactant Micelles

et al. 2010

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“…Curcumin exhibits a variety of biological and photochemical properties, including antioxidant, anti-inflammatory, and anticancer activity. [2][3][4][5][6] Recently, it was also established that curcumin has the ability to prevent protein aggregation in debilitating diseases such as Alzheimer's and Parkinson's. 7,8 Curcumin has two tautomeric forms, namely -diketone and keto-enol.…”

Section: Introductionmentioning

confidence: 99%

Excited-State Intramolecular Hydrogen Atom Transfer and Solvation Dynamics of the Medicinal Pigment Curcumin

et al. 2009

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The potential use of the naturally occurring yellow-orange pigment curcumin as a photodynamic therapy agent is one of the most exciting applications of this medicinal compound. Although subnanosecond spectroscopy has been used to investigate the photophysical processes of curcumin, the time resolution is insufficient to detect important and faster photoinduced processes, including solvation and excited-state intramolecular hydrogen atom transfer (ESIHT). In this study, the excited-state photophysics of curcumin is studied by means of ultrafast fluorescence upconversion spectroscopy. The results show two decay components in the excited-state kinetics with time scales of 12−20 ps and ∼100 ps in methanol and ethylene glycol. The resulting prominent isotope effect in the long component upon deuteration indicates that curcumin undergoes ESIHT in these solvents. The short component (12−20 ps) is insensitive to deuteration, and multiwavelength fluorescence upconversion results show that this decay component is due to solvation of excited-state curcumin. KeywordsAtomic spectroscopy, deuterium, ethylene, ethylene glycol, fluorescence, free radical reactions, hydrogen, methanol, photodynamic therapy, solvation, curcumin, decay components, deuteration, excited-state, fluorescence upconversion, intramolecular hydrogens, isotope effects, photophysics The potential use of the naturally occurring yellow-orange pigment curcumin as a photodynamic therapy agent is one of the most exciting applications of this medicinal compound. Although subnanosecond spectroscopy has been used to investigate the photophysical processes of curcumin, the time resolution is insufficient to detect important and faster photoinduced processes, including solvation and excited-state intramolecular hydrogen atom transfer (ESIHT). In this study, the excited-state photophysics of curcumin is studied by means of ultrafast fluorescence upconversion spectroscopy. The results show two decay components in the excited-state kinetics with time scales of 12-20 ps and ∼100 ps in methanol and ethylene glycol. The resulting prominent isotope effect in the long component upon deuteration indicates that curcumin undergoes ESIHT in these solvents. The short component (12-20 ps) is insensitive to deuteration, and multiwavelength fluorescence upconversion results show that this decay component is due to solvation of excited-state curcumin.

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“…Curcumin‐induced apoptosis by downregulating anti‐apoptotic Bcl‐2 and upregulating proapoptotic Bax, thereby decreasing the Bcl‐2/Bax ratios. Our result about Bcl‐2/Bax ratio is in agreement with previous studies (Shi et al., 2006; Song et al., 2005). Therefore, to confirm the role of curcumin in apoptosis, we further quantitated the expression of p53, bax, and bcl‐2.…”

Section: Discussionmentioning

confidence: 99%

A study on effect of curcumin on anticerebral aneurysm in the male albino rats

Miao

Wang

et al. 2017

Brain and Behavior

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IntroductionThis study investigated the curcumin effect on the cerebral aneurysm. Apoptosis is known to play a fundamental role in the pathogenesis of a cerebral aneurysm. Therefore, we investigated the effect of curcumin on apoptosis of smooth muscle cells of a cerebral aneurysm‐induced male albino rats.MethodsIn this study, the cerebral aneurysm has been induced in the male albino rats by the CaCl2 administration. After cerebral aneurysm induction, smooth muscle cells were isolated. Cells were treated with curcumin (25 & 50 mg/kg bwt) for 48 hr.ResultsCurcumin reduced altered mitochondrial morphology significantly, evidenced through fluorescence and confocal study. Curcumin treatment reduced the expression of p53, caspase‐3, and bax/bxl‐2 ratio significantly. Curcumin treatment also reversed the cellular architecture of smooth muscle cell wall significantly. Fluorescence and the confocal study confirmed the reduction in apoptosis in a cerebral aneurysm‐induced smooth muscle cells of male albino rats.ConclusionTaking all these data together, it may suggest that the curcumin could significantly reduce the CaCl2‐induced cerebral aneurysm through the inhibition of cell apoptosis in the cells.

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Antiproliferation and apoptosis induced by curcumin in human ovarian cancer cells

Cited by 200 publications

References 28 publications

Excited-State Intramolecular Hydrogen Atom Transfer of Curcumin in Surfactant Micelles

Excited-State Intramolecular Hydrogen Atom Transfer of Curcumin in Surfactant Micelles

Excited-State Intramolecular Hydrogen Atom Transfer and Solvation Dynamics of the Medicinal Pigment Curcumin

A study on effect of curcumin on anticerebral aneurysm in the male albino rats

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